Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 365,405 Δ93 bp coding (33‑125/3075 nt) lacZ ← beta‑D‑galactosidase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 365405 365497 93 230 [0] [0] 227 lacZ beta‑D‑galactosidase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3654040 (0.000)225 (1.420) 107/150 0.0 100% coding (126/3075 nt) lacZ beta‑D‑galactosidase
?NC_000913 365498 = 0 (0.000)coding (32/3075 nt) lacZ beta‑D‑galactosidase

Only 100 of 230 total aligned reads displayed.

TGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTC  >  NC_000913/365329‑365478
                                                                           |                                                                          
 ggTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGccc                                                                           >  2:2670266/1‑75 (MQ=255)
 ggTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCa                                                                           >  1:5515401‑M1/1‑75 (MQ=255)
 ggTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCa                                                                           <  2:2023568‑M1/76‑2 (MQ=255)
 ggTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCa                                                                           <  2:1523996‑M1/76‑2 (MQ=255)
  gTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCac                                                                          <  1:5472405‑M1/76‑3 (MQ=255)
  gTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCac                                                                          <  2:2002333‑M1/76‑3 (MQ=255)
  gTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCac                                                                          <  1:1793650‑M1/76‑3 (MQ=255)
   tGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacg                                                                         >  2:1589392‑M1/1‑73 (MQ=255)
   tGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacg                                                                         <  1:2957059‑M1/76‑4 (MQ=255)
    gCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacga                                                                        <  2:3310258‑M1/76‑5 (MQ=255)
     ccGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgac                                                                       <  1:2154680‑M1/76‑6 (MQ=255)
     ccGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgac                                                                       <  2:3010727‑M1/76‑6 (MQ=255)
      cGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacg                                                                      >  1:2253111‑M1/1‑70 (MQ=255)
      cGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacg                                                                      >  2:4181268‑M1/1‑70 (MQ=255)
       ggAGACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacgg                                                                     <  2:134702‑M1/76‑8 (MQ=255)
       ggAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacgg                                                                     >  2:5532256‑M1/1‑69 (MQ=255)
        gAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggc                                                                    >  1:1610371‑M1/1‑68 (MQ=255)
         aaaCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggcc                                                                   <  2:3068989‑M1/76‑10 (MQ=255)
         aaaCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggcc                                                                   <  2:2865685‑M1/76‑10 (MQ=255)
          aaCCAGCCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggcca                                                                  <  1:102474‑M1/76‑11 (MQ=255)
            ccAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagt                                                                >  1:1894083‑M1/1‑64 (MQ=255)
             cgggcaAAGCCCCATTCGCCATTCAGGCGGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtg                                                               <  2:5786468‑M1/74‑14 (MQ=255)
             cAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtg                                                               <  1:4115995‑M1/76‑14 (MQ=255)
             cAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtg                                                               <  1:3301360‑M1/76‑14 (MQ=255)
              aGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtga                                                              <  1:5146738‑M1/76‑15 (MQ=255)
              aGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtga                                                              >  2:1243158‑M1/1‑62 (MQ=255)
              aGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtga                                                              <  1:1090895‑M1/76‑15 (MQ=255)
               ggCAAAGCGCCAGTCGCCATGCAGGCTGCGCAACTGTGGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaa                                                             <  2:5040766‑M1/76‑16 (MQ=255)
                 gaaagGGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatc                                                           <  2:1715013‑M1/75‑18 (MQ=255)
                  aacgcgccatTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatcc                                                          <  1:2270681‑M1/73‑19 (MQ=255)
                  aaaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatcc                                                          <  1:3727073‑M1/76‑19 (MQ=255)
                  aaaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatac                                                          >  1:1260615‑M1/1‑58 (MQ=255)
                  aaaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggc                                                                    <  1:1971756‑M1/66‑9 (MQ=255)
                   aggcgccgTTCGCCATGCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccg                                                         <  2:3561264‑M1/74‑20 (MQ=255)
                   aaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccg                                                         >  1:3160608‑M1/1‑57 (MQ=255)
                    aGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgt                                                        <  2:3919633‑M1/76‑21 (MQ=255)
                     gcgcCATTCGCCATTCAGGCTGCGCAACTGTGGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgta                                                       <  1:879056‑M1/76‑22 (MQ=255)
                     gcgcCATCCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgta                                                       <  1:5031703‑M1/76‑22 (MQ=255)
                      cgccattcgcccttCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGGGACGGCCacgccggccagggaacccgtaa                                                      >  1:3829311‑M1/1‑54 (MQ=255)
                      cgccattcgccattCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccggaa                                                      >  2:1951886‑M1/1‑54 (MQ=255)
                      cgccatgcgccattCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaa                                                      <  2:4471563‑M1/76‑23 (MQ=255)
                       gccattcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCccgacggccagtgaatccgtaat                                                     >  2:5101154‑M1/1‑53 (MQ=255)
                       gccattcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTCCGGGCCacgacggccagtgaatccgtaat                                                     >  1:3910902‑M1/1‑53 (MQ=255)
                        ccattcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatc                                                    >  2:2119086‑M1/1‑52 (MQ=255)
                          attcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcat                                                  <  1:971094‑M1/76‑27 (MQ=255)
                          attcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCaccacggccagtgaatccgcaatcag                                                  >  2:2291423‑M1/1‑50 (MQ=255)
                           ttcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatg                                                 >  1:3452883‑M1/1‑49 (MQ=255)
                            tcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatgg                                                >  1:5654382‑M1/1‑48 (MQ=255)
                             cgccattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggt                                               >  1:4976254‑M1/1‑47 (MQ=255)
                              cccattcgGGCTGCGCAACTGTGGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtc                                              <  1:69501‑M1/75‑31 (MQ=255)
                              cccattcaGGCTGCGCAGTGGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtc                                              <  2:2795190‑M1/75‑31 (MQ=255)
                                cattcGGGCTGCGCAACTGTGGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcat                                            <  2:3268187‑M1/76‑33 (MQ=255)
                                cattcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcat                                            <  1:1873891‑M1/76‑33 (MQ=255)
                                 tttcaGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcata                                           <  1:5607810‑M1/75‑34 (MQ=255)
                                  ttcAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagagaatgcgtaatcatggtgataa                                          >  1:2413641‑M1/1‑42 (MQ=255)
                                    gagGGTGGGCAACGGTGGGGAGGGGCGATCGGTGGGGGCCacgacggccagtgaatccgtaatcatggtcatagct                                        <  2:5112412‑M1/75‑37 (MQ=255)
                                    cAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagct                                        <  1:2047914‑M1/76‑37 (MQ=255)
                                    cAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagct                                        <  1:2817868‑M1/76‑37 (MQ=255)
                                     gggcTGCGCAACTGTGGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctg                                       <  1:2666115‑M1/75‑38 (MQ=255)
                                      ggCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgt                                      >  2:4627855‑M1/1‑38 (MQ=255)
                                       gCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatcgctggt                                     >  2:5682693‑M1/1‑37 (MQ=255)
                                        cTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgttt                                    <  1:3496915‑M1/76‑41 (MQ=255)
                                        cTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgttt                                    <  1:4203509‑M1/76‑41 (MQ=255)
                                          gcgcAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcc                                  <  1:2729529‑M1/76‑43 (MQ=255)
                                           cgcAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcct                                 >  1:2127283‑M1/1‑33 (MQ=255)
                                           cgcAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttccc                                 >  2:5192143‑M1/1‑33 (MQ=255)
                                            gcAACTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctg                                >  2:621185‑M1/1‑32 (MQ=255)
                                              aattgtggggAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtg                              <  1:4689554‑M1/73‑47 (MQ=255)
                                              aaCTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctctg                              >  2:548033‑M1/1‑30 (MQ=255)
                                                cggttGGGAGGGGCGATCGGTGCGGGCCgcgccggccagtgaatccgtaatcatggtcatagctgtttcctgtgtg                            <  2:3676526‑M1/74‑49 (MQ=255)
                                                cTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtg                            >  1:45926‑M1/1‑28 (MQ=255)
                                                cTGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtg                            >  1:5850998‑M1/1‑28 (MQ=255)
                                                 tGTTGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtga                           >  1:398712‑M1/1‑27 (MQ=255)
                                                  gtggggaagGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaa                          <  1:2999853‑M1/73‑51 (MQ=255)
                                                   ttGGGAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaa                         >  1:2768061‑M1/1‑25 (MQ=255)
                                                     gggAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaatt                       >  1:1373490‑M1/1‑23 (MQ=255)
                                                     gggAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaatt                       <  1:4772159‑M1/76‑54 (MQ=255)
                                                     gggAAGGGCGATCGGTGCGGACCacgacggccagtgaatccgtaatcatggtcatagctggtttctgtgtgaaatt                       >  1:1131562‑M1/1‑23 (MQ=255)
                                                      ggAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgcttcctgtgtgaaattg                      >  2:3169990‑M1/1‑22 (MQ=255)
                                                       gAAGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgt                     >  2:4857729‑M1/1‑21 (MQ=255)
                                                        aaGGGCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgtt                    >  1:2209956‑M1/1‑20 (MQ=255)
                                                         ggggcGATCGGTGCGGGCCacgacggccagtgaatctgtaatcatggtcatagctgtttcctgtgtgaaattgtta                   <  2:3755371‑M1/75‑58 (MQ=255)
                                                         ggggcGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgtta                   <  2:2543689‑M1/75‑58 (MQ=255)
                                                          gggCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttgt                  <  2:4081778‑M1/76‑59 (MQ=255)
                                                          gggCGATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttat                  <  2:2209956‑M1/76‑59 (MQ=255)
                                                           ggCGATGGGTGGGGGCCacgccggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatc                 <  1:61993‑M1/76‑60 (MQ=255)
                                                           ggCGAGCGGTGCGGGCCagaacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatc                 <  1:4356239‑M1/76‑60 (MQ=255)
                                                              gATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgc              >  2:2783649‑M1/1‑14 (MQ=255)
                                                              gATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgc              >  2:130497‑M1/1‑14 (MQ=255)
                                                              gATCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgc              <  1:1502405‑M1/76‑63 (MQ=255)
                                                               atgggtggGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgct             <  2:834376‑M1/73‑64 (MQ=255)
                                                               aTCGGTGCGGGCCgcgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgct             <  2:4128793‑M1/76‑64 (MQ=255)
                                                               aTCGGTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgct             >  2:1058787‑M1/1‑13 (MQ=255)
                                                                tCGGTGCGGGCCacgacggccagtgaatccgtactcatggtcatagctgtttcctgtgtgaaatcgttatccgctc            >  2:2825456‑M1/1‑12 (MQ=255)
                                                                gcgGGGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctc            <  2:1610371‑M1/75‑65 (MQ=255)
                                                                   gTGCGGGCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcaca         >  1:4200747‑M1/1‑9 (MQ=255)
                                                                     gCGGGCCacgccggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaat       <  1:3152993‑M1/76‑70 (MQ=255)
                                                                      ggggcCacgacggccagtgaatccgtaatcatggttatagctgtttcctgtgtgaaattgttatccgctcacaatt      <  2:2483660‑M1/75‑71 (MQ=255)
                                                                        ggCCacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattcc    <  2:3319673‑M1/76‑73 (MQ=255)
                                                                          ccacgacggccagtgaatccgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccac  >  2:1675340‑M1/1‑2 (MQ=255)
                                                                           |                                                                          
TGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTC  >  NC_000913/365329‑365478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑