BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	365,405	Δ93 bp	coding (33‑125/3075 nt)	lacZ ←	beta‑D‑galactosidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	365405	365497	93	230 [0]	[0] 227	lacZ	beta‑D‑galactosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 365404	0 (0.000)	225 (1.420)	107/150	0.0	100%	coding (126/3075 nt)	lacZ	beta‑D‑galactosidase
*	?	NC_000913	365498 =	0 (0.000)	225 (1.420)	107/150	0.0	100%	coding (32/3075 nt)	lacZ	beta‑D‑galactosidase

Only 100 of 227 total aligned reads displayed.

CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACAC  >  NC_000913/365423‑365573

ggtgccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcca                                                                             <  2:2023568‑M2/1‑1 (MQ=255)

ggtgccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcca                                                                             <  2:1523996‑M2/1‑1 (MQ=255)

 gtgccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccac                                                                            <  1:3780188‑M2/2‑1 (MQ=255)

 gtgccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccac                                                                            <  2:2002333‑M2/2‑1 (MQ=255)

 gtgccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccac                                                                            <  1:4185073‑M2/2‑1 (MQ=255)

    ccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccacgac                                                                         >  2:3152993‑M2/72‑76 (MQ=255)

    ccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccacgac                                                                         <  1:4201574‑M2/5‑1 (MQ=255)

    ccggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccacgac                                                                         <  1:2154680‑M2/5‑1 (MQ=255)

     cggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccacgacg                                                                        >  1:2253111‑M2/71‑76 (MQ=255)

      ggagaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACgg                                                                       <  2:134702‑M2/7‑1 (MQ=255)

      ggaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACgg                                                                       >  2:5532256‑M2/70‑76 (MQ=255)

       gaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGc                                                                      <  1:829035‑M2/8‑1 (MQ=255)

       gaaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGc                                                                      >  1:1610371‑M2/69‑76 (MQ=255)

        aaaccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGcc                                                                     <  1:5476456‑M2/9‑1 (MQ=255)

         aaccagccaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCa                                                                    <  1:102474‑M2/10‑1 (MQ=255)

          accaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAg                                                                   <  1:4917136‑M2/11‑1 (MQ=255)

          accaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAg                                                                   <  2:2966630‑M2/11‑1 (MQ=255)

           ccgggcaaggcgccatgcgccattcaggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGt                                                                  <  2:321760‑M2/12‑1 (MQ=255)

           ccaggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGt                                                                  >  1:1894083‑M2/65‑76 (MQ=255)

            cgggcaaagccccattcgccattcaggcggcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTg                                                                 <  2:5786468‑M2/13‑1 (MQ=255)

            caggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTg                                                                 <  1:4227605‑M2/13‑1 (MQ=255)

            caggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTg                                                                 <  1:3301360‑M2/13‑1 (MQ=255)

            caggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTg                                                                 >  2:2106201‑M2/64‑76 (MQ=255)

            cagccaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTg                                                                 <  1:5671393‑M2/13‑1 (MQ=255)

             gggcaaggcgccattcgccattcaggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                <  2:2717670‑M2/14‑1 (MQ=255)

             agggaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                <  2:1582943‑M2/14‑1 (MQ=255)

             aggcaaagcgcctttcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                <  1:5780653‑M2/14‑1 (MQ=255)

             aggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                <  1:5146738‑M2/14‑1 (MQ=255)

             aggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                >  2:1243158‑M2/63‑76 (MQ=255)

             aggcaaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGa                                                                <  1:1090895‑M2/14‑1 (MQ=255)

              ggcaaagcgccagtcgccatgcaggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGaa                                                               <  2:5040766‑M2/15‑1 (MQ=255)

                caaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATc                                                             >  1:3476472‑M2/60‑76 (MQ=255)

                 aacgcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATcc                                                            <  1:2270681‑M2/18‑1 (MQ=255)

                 aaagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGaatac                                                            >  1:1260615‑M2/59‑74 (MQ=255)

                  aggcgccgttcgccatgcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCg                                                           <  2:3561264‑M2/19‑1 (MQ=255)

                  aagcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCg                                                           >  1:1988467‑M2/58‑76 (MQ=255)

                   agcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGt                                                          <  2:3919633‑M2/20‑1 (MQ=255)

                    gcgccatccgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTa                                                         <  1:5031703‑M2/21‑1 (MQ=255)

                     cgccatgcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTaa                                                        <  2:4471563‑M2/22‑1 (MQ=255)

                      gccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccCCGACGGCCAGTGAATCCGTAAt                                                       >  2:5101154‑M2/54‑76 (MQ=255)

                       ccattcgccattcgggctgcgcaactgttgggaagggcgatgggtgggggccACGACGGCCAGTGAATCCGTAATc                                                      <  2:2814918‑M2/24‑1 (MQ=255)

                        cattcgccattcaggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCa                                                     <  1:5194677‑M2/25‑1 (MQ=255)

                         attcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCAt                                                    <  1:971094‑M2/26‑1 (MQ=255)

                         attcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACCACGGCCAGTGAATCCGCAATcag                                                    >  2:2291423‑M2/51‑75 (MQ=255)

                          ttcgccattcaggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATg                                                   <  2:4068856‑M2/27‑1 (MQ=255)

                           tcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATgg                                                  >  2:12557‑M2/49‑76 (MQ=255)

                           tcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATgg                                                  <  2:2522007‑M2/28‑1 (MQ=255)

                            cgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGt                                                 >  2:4870536‑M2/48‑76 (MQ=255)

                             gccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTc                                                <  1:2623248‑M2/30‑1 (MQ=255)

                             gccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTc                                                >  1:3094035‑M2/47‑76 (MQ=255)

                             cccattcgggctgcgcaactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTc                                                <  1:69501‑M2/30‑1 (MQ=255)

                             cccattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTc                                                <  1:2789397‑M2/30‑1 (MQ=255)

                              ccatccaggctgcgcaactgtggggaggggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCa                                               <  1:4046556‑M2/31‑1 (MQ=255)

                               cattcaggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCAt                                              <  1:1873891‑M2/32‑1 (MQ=255)

                                   gagggtgggcaacggtggggaggggcgatcggtgggggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCt                                          <  2:5112412‑M2/36‑1 (MQ=255)

                                    aggctgcgcaactgttgggaagggcgagcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGATg                                         >  2:1760898‑M2/40‑76 (MQ=255)

                                     ggctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGt                                        >  2:4627855‑M2/39‑76 (MQ=255)

                                      gctgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATCGctggt                                       >  2:5682693‑M2/38‑74 (MQ=255)

                                        tgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATg                                                   <  1:1710454‑M2/27‑1 (MQ=255)

                                        tgcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATg                                                   >  2:1710454‑M2/36‑62 (MQ=255)

                                         gcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTcc                                    >  2:3319258‑M2/35‑76 (MQ=255)

                                         gcgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTcc                                    <  1:2729529‑M2/42‑1 (MQ=255)

                                          cgcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCt                                   >  1:2127283‑M2/34‑76 (MQ=255)

                                           gcaactgttgggaagggcgttcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtg                                  <  2:3278418‑M2/44‑1 (MQ=255)

                                           gcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTttccct                                  >  2:2784793‑M2/33‑74 (MQ=255)

                                           gcaactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtg                                  >  2:621185‑M2/33‑76 (MQ=255)

                                            caacggtggggaagggcgatcggggcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgt                                 <  2:2127283‑M2/45‑1 (MQ=255)

                                             aattgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtg                                <  1:4689554‑M2/46‑1 (MQ=255)

                                             aactgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtctg                                >  2:548033‑M2/31‑76 (MQ=255)

                                             aactgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtg                                <  2:2449102‑M2/46‑1 (MQ=255)

                                              actgttgggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgt                               >  1:3156500‑M2/30‑76 (MQ=255)

                                              actgtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCtgtgt                               <  2:3053027‑M2/47‑1 (MQ=255)

                                                 gtggggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGaa                            <  1:2999853‑M2/50‑1 (MQ=255)

                                                   ggggaggtgcgactggtgcggcccACGACGGCCCGTGAATCCGTAATCATGGTCATAGCTTTTTCCTGTGTGAAAt                          <  1:553369‑M2/52‑1 (MQ=255)

                                                    gggaggggcgatcggggcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAtt                         <  2:1594840‑M2/53‑1 (MQ=255)

                                                    gggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAtt                         <  1:2746444‑M2/53‑1 (MQ=255)

                                                    gggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAtt                         >  1:1373490‑M2/24‑76 (MQ=255)

                                                    gggaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAtt                         <  1:4772159‑M2/53‑1 (MQ=255)

                                                    gggaagggcgatcggtgcggaccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGGTTTCTGTGTGAAAtt                         >  1:1131562‑M2/24‑76 (MQ=255)

                                                      gaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTTCTGTGTGGAATTGt                       >  1:3832330‑M2/22‑76 (MQ=255)

                                                      gaagggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGt                       <  1:2937736‑M2/55‑1 (MQ=255)

                                                        ggggcgatcggtgcgggccACGACGGCCAGTGAATCTGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTa                     <  2:3755371‑M2/57‑1 (MQ=255)

                                                        agggcggtgggtgcgggccACGCCGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTa                     <  2:530018‑M2/57‑1 (MQ=255)

                                                        agggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTa                     <  1:3982328‑M2/57‑1 (MQ=255)

                                                        agggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTa                     <  1:3569033‑M2/57‑1 (MQ=255)

                                                          ggcgatgggtgggggccACGCCGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATc                   <  1:61993‑M2/59‑1 (MQ=255)

                                                          ggcgatcgttgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATc                   <  1:2201802‑M2/59‑1 (MQ=255)

                                                          ggcgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATc                   <  1:4524575‑M2/59‑1 (MQ=255)

                                                            cgatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCg                 >  2:4320583‑M2/16‑76 (MQ=255)

                                                             gatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                <  1:1502405‑M2/62‑1 (MQ=255)

                                                             gatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                >  1:564987‑M2/15‑76 (MQ=255)

                                                             gatcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGc                >  2:2832085‑M2/15‑76 (MQ=255)

                                                              atgggtgggggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt               <  2:834376‑M2/63‑1 (MQ=255)

                                                              atcggtgcgggccGCGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt               <  2:4128793‑M2/63‑1 (MQ=255)

                                                              atcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCt               >  2:1058787‑M2/14‑76 (MQ=255)

                                                               tcggtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTc              >  2:3400258‑M2/13‑76 (MQ=255)

                                                                  gtgcgggcgACGTCGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCgctctca           <  1:1952140‑M2/67‑4 (MQ=255)

                                                                  gtgcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTcaca           >  1:2329056‑M2/10‑76 (MQ=255)

                                                                    gcgggccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAt         <  1:3447836‑M2/69‑1 (MQ=255)

                                                                         ccACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCcac    >  2:1675340‑M2/3‑76 (MQ=255)

CTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACAC  >  NC_000913/365423‑365573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`