breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 649 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,008,398 | 4 | . | A | 100.0% | 11.4 / NA | 6 | E161K (GAA→AAA) | b4555 | hypothetical protein |
* | NC_000913 | 4,293,959 | 2 | . | G | 100.0% | 11.4 / NA | 11 | intergenic (+586/+56) | gltP/yjcO | glutamate‑aspartate symport protein/hypothetical protein |
* | NC_000913 | 246,747 | 0 | G | T | 73.8% | 515.2 / 128.8 | 303 | S12S (TCG→TCT) | yafL | putative lipoprotein |
* | NC_000913 | 366,797 | 0 | G | A | 70.6% | 797.8 / inf | 550 | intergenic (‑54/+14) | lacI/mhpR | LacI/transcriptional regulator for mhp operon |
* | NC_000913 | 301,410 | 0 | C | T | 70.2% | 867.4 / inf | 584 | E130K (GAG→AAG) | yagT | putative xanthine dehydrogenase |
* | NC_000913 | 244,133 | 0 | G | A | 64.9% | 410.8 / inf | 388 | intergenic (+12/‑194) | gmhA/yafJ | phosphoheptose isomerase/putative amidotransferase |
* | NC_000913 | 2,724,645 | 0 | T | G | 59.3% | ‑3.5 / 11.1 | 27 | noncoding (2560/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 3,192,873 | 0 | T | C | 55.1% | 21.7 / 162.1 | 136 | intergenic (+332/+88) | b3051/b3052 | putative membrane protein/putative kinase |
* | NC_000913 | 2,724,651 | 0 | A | C | 48.3% | ‑5.7 / 12.1 | 29 | noncoding (2554/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 3,192,891 | 0 | C | T | 48.0% | 8.0 / 202.5 | 149 | intergenic (+350/+70) | b3051/b3052 | putative membrane protein/putative kinase |
* | NC_000913 | 2,783,976 | 0 | A | C | 46.4% | 44.9 / 38.3 | 69 | intergenic (‑119/‑441) | ileY/b2654 | tRNA‑Ile/hypothetical protein |
* | NC_000913 | 576,265 | 0 | T | G | 45.7% | 6.8 / 24.9 | 35 | intergenic (‑157/‑356) | nmpC/ybcR | outer membrane porin protein/hypothetical protein |
* | NC_000913 | 3,192,609 | 0 | A | G | 44.5% | 91.8 / 185.1 | 174 | intergenic (+68/+352) | b3051/b3052 | putative membrane protein/putative kinase |
* | NC_000913 | 4,279,347 | 0 | T | G | 43.9% | 41.1 / 30.2 | 68 | intergenic (+139/+15) | yjcE/yjcF | hypothetical protein/hypothetical protein |
* | NC_000913 | 1,648,172 | 0 | T | G | 43.9% | 24.5 / 12.7 | 41 | V24G (GTA→GGA) | ydfE | hypothetical protein |
* | NC_000913 | 207,482 | 0 | T | G | 42.9% | 53.0 / 24.1 | 56 | V786G (GTA→GGA) | dnaE | DNA polymerase III alpha subunit |
* | NC_000913 | 1,565,413 | 0 | T | G | 42.0% | 32.5 / 27.0 | 50 | intergenic (‑249/+115) | b1490/b1491 | hypothetical protein/hypothetical protein |
* | NC_000913 | 576,271 | 0 | A | T | 41.2% | 18.8 / 28.2 | 35 | intergenic (‑163/‑350) | nmpC/ybcR | outer membrane porin protein/hypothetical protein |
* | NC_000913 | 4,090,715 | 0 | T | G | 41.1% | 56.9 / 26.8 | 74 | Y102S (TAC→TCC) | yiiL | hypothetical protein |
* | NC_000913 | 4,592,172 | 0 | T | G | 40.4% | 29.0 / 20.9 | 47 | T41P (ACC→CCC) | yjiZ | putative transport protein |
Marginal new junction evidence (lowest skew 10 of 18 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 244134 = | 138 (1.010) | 20 (0.150) +T |
7/148 | 9.7 | 12.6% | intergenic (+13/‑193) | gmhA/yafJ | phosphoheptose isomerase/putative amidotransferase |
? | NC_000913 | = 376715 | 143 (1.050) | intergenic (+180/+44) | yaiL/yaiM | nucleoprotein/polynucleotide‑associated enzyme/putative esterase | |||||
* | ? | NC_000913 | 2905021 = | 139 (1.020) | 5 (0.040) | 5/134 | 10.2 | 3.9% | coding (943/1299 nt) | eno | enolase |
? | NC_000913 | 2905029 = | 121 (0.990) | coding (935/1299 nt) | eno | enolase | |||||
* | ? | NC_000913 | 356700 = | 626 (4.590) | 124 (0.920) +C |
6/148 | 10.2 | 21.6% | intergenic (+22/+315) | codA/cynR | cytosine deaminase/cyn operon positive regulator |
? | NC_000913 | = 357098 | 286 (2.090) | coding (817/900 nt) | cynR | cyn operon positive regulator | |||||
* | ? | NC_000913 | = 285707 | 594 (4.350) | 8 (0.060) | 5/146 | 10.6 | 2.2% | coding (1089/1383 nt) | yagG | putative permease |
? | NC_000913 | = 4155330 | 133 (1.000) | coding (902/1374 nt) | argH | argininosuccinate lyase | |||||
* | ? | NC_000913 | 274105 = | NA (NA) | 4 (0.030) | 4/130 | 10.6 | NA | coding (237/1017 nt) | trs51 | IS5 transposase |
? | NC_000913 | 274115 = | NA (NA) | coding (227/1017 nt) | trs51 | IS5 transposase | |||||
* | ? | NC_000913 | = 244753 | 485 (3.550) | 4 (0.030) | 4/138 | 10.9 | 0.89% | coding (427/768 nt) | yafJ | putative amidotransferase |
? | NC_000913 | = 244754 | 450 (3.590) | coding (428/768 nt) | yafJ | putative amidotransferase | |||||
* | ? | NC_000913 | = 276631 | 756 (5.540) | 4 (0.030) | 4/144 | 11.1 | 0.55% | coding (693/933 nt) | yagD | YagD |
? | NC_000913 | = 276645 | 710 (5.420) | coding (707/933 nt) | yagD | YagD | |||||
* | ? | NC_000913 | 4540250 = | 48 (0.350) | 3 (0.030) | 3/132 | 11.3 | 5.9% | intergenic (+49/‑433) | fimE/fimA | FimE/major type 1 subunit fimbrin |
? | NC_000913 | 4540564 = | 54 (0.450) | intergenic (+363/‑119) | fimE/fimA | FimE/major type 1 subunit fimbrin | |||||
* | ? | NC_000913 | 4330255 = | 163 (1.190) | 3 (0.030) | 3/132 | 11.3 | 2.1% | coding (596/1092 nt) | basS | BasS |
? | NC_000913 | = 4496171 | NA (NA) | coding (377/411 nt) coding (9/906 nt) |
yi216 yi226 |
IS2 hypothetical protein IS2 hypothetical protein |
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* | ? | NC_000913 | 831580 = | 103 (0.750) | 3 (0.020) | 3/136 | 11.4 | 2.7% | intergenic (+121/+111) | rhlE/ybiA | putative ATP‑dependent RNA helicase/hypothetical protein |
? | NC_000913 | 3596127 = | 119 (0.960) | intergenic (+129/+59) | yhhK/b3460 | hypothetical protein/LivJ |