New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 84615269 (0.850)7 (0.110)
+TTTATGCGTTT		 3/76 NT 10.2% intergenic (‑250/+11) ybiO/glnQ predicted mechanosensitive channel/glutamine transporter subunit
?W3110S.gb 2944517 = 90 (1.110)intergenic (‑19/+220) ygdL/mltA conserved hypothetical protein/membrane‑bound lytic murein transglycosylase A

TGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/846122‑846152
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGGCGAGGTTATCCCTCACCCTAA  >  W3110S.gb/2944517‑2944541
                               |||||||||||                         
TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATGCGTTT                           <  1:4200096/42‑1
TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATGCGTT                            <  1:4260504/41‑1
TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATGCGTT                            <  1:5899669/41‑1
TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATGCGTT                            <  1:7010831/41‑1
       CTGATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCACCCTAA  >  1:1362753/1‑60
       CTGATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCACCCTAA  >  1:4886341/1‑60
       CTGATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTACCCCTCACCCTAA  >  1:7126223/1‑60
         GATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCA        <  1:2491129/52‑1
         GATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCA        <  1:5112741/52‑1
              GCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCA        >  1:1743673/1‑47
              GCGTAGCGCATCAGGCATTTATGCGTTTAGGGCGAGGTTATCCCTCA        >  1:3066314/1‑47
                               |||||||||||                         
TGTAGGCCTGATAAGCGTAGCGCATCAGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/846122‑846152
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGGCGAGGTTATCCCTCACCCTAA  >  W3110S.gb/2944517‑2944541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.