New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 4523153140 (1.720)9 (0.120) 3/94 NT 5.8% intergenic (‑236/‑56) fecI/insA RNA polymerase, sigma 19 factor/IS1 repressor protein InsA
?W3110S.gb = 4523173 159 (2.040)intergenic (‑256/‑36) fecI/insA RNA polymerase, sigma 19 factor/IS1 repressor protein InsA

TGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4523082‑4523153
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAGGATGTCAA  <  W3110S.gb/4523173‑4523119
                                                                                                                               
TGTCGGTCAGAATGACTAATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          <  1:2440550/71‑1
 GTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTC                                                         >  1:3679653/1‑71
     GTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          <  1:5840773/66‑1
                    GATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTC                                                         >  1:1199404/1‑52
                    GATGTGGTCTGCTATTATTGACATCCTCACTGCCATAAAGGATGGGGATTTC                                                         >  1:7797271/1‑52
                     ATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          <  1:1473971/50‑1
                     ATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          <  1:5968086/50‑1
                                  TTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          >  1:3841491/1‑37
                                  TTATTGACATCCTCACTGCCCTAAAGGATGGGGATTT                                                          >  1:6108161/1‑37
                                            CCTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATTACCG                                    <  1:3657718/49‑1
                                             CTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATT                                        <  1:3177616/44‑1
                                             CTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATT                                        <  1:2995279/44‑1
                                             CTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATT                                        <  1:6616148/44‑1
                                             CTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATT                                        <  1:1549761/44‑1
                                             CTCACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCATT                                        <  1:969602/44‑1
                                                ACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCAT                                         >  1:4939977/1‑40
                                                ACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCAT                                         >  1:2293927/1‑40
                                                ACTGCCCTAAAGGATGGGGATTTCAGTAAGTTGGCAGCAT                                         >  1:7223087/1‑40
                                                                      TCAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAG          >  1:1926378/1‑49
                                                                       CAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAGGATGTCAA  <  1:4502578/56‑1
                                                                       CAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAGGATGTCAA  <  1:2926455/56‑1
                                                                       CAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAGGATGTCAA  <  1:6623382/56‑1
                                                                                                                               
TGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4523082‑4523153
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcAGTAAGTTGGCAGCATTACCGAAATCCCCATCCTTTAGGGCAGTGAGGATGTCAA  <  W3110S.gb/4523173‑4523119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.