New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	713380 =	24 (1.290)	21 (1.230)	15/92	NT	53.8%	intergenic (‑156/‑29)	ybfF/seqA	conserved hypothetical protein/regulatory protein for replication initiation
	?	W3110S.gb	= 4125340	14 (0.820)					coding (836/837 nt)	dam	DNA adenine methylase

CAATATAGCTGTAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/713451‑713380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaatTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTG  <  W3110S.gb/4125340‑4125272                                                                                                                                                CAATATAGCTGTAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAA                                                                         <  1:928820/71‑1  AATATAGCTGTAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTG                                                                           <  1:10463/68‑1            TAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTC                                                               <  1:1429794/70‑1            TAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTC                                                               <  1:378147/70‑1             AGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCtc                                                             >  1:593345/1‑69              GAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGC                                                             <  1:417820/70‑1                      CATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTC                                                               <  1:1754331/60‑1                      CATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAAT                                                                        <  1:274531/51‑1                      CATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAAT                                                                        <  1:1425429/51‑1                       ATCAACTTGAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGC                                                             <  1:437933/61‑1                       ATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACG                                                   <  1:1286553/71‑1                       ATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACG                                                   <  1:163004/71‑1                             TTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTA                                                                      >  1:120270/1‑46                                 ATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TT                                        >  1:1184457/1‑71                                  TCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑T                                         >  1:155640/1‑69                                     TTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCG                                                            <  1:1738908/48‑1                                            CTTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAAC                                                    >  1:88762/1‑49                                             TTAATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGC                              >  1:1525720/1‑69                                                ATCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCA                            <  1:191657/68‑1                                                 TCCAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACA                                  <  1:776531/61‑1                                                   CAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTGCTGGCTTTGTACAAA‑CCAGGAGTc                      <  1:691195/71‑2                                                   CAGTGCAGTGTCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGT                       <  1:849542/70‑1                                                             TCTTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TT                                        <  1:1369480/43‑1                                                               TTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTT          >  1:189393/1‑71                                                               TTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTT                                       <  1:443783/42‑1                                                               TTAGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTT                                       <  1:829688/42‑1                                                                 AGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTA        >  1:1336143/1‑71                                                                 AGCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTT           <  1:432819/68‑1                                                                  GCTGAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTAC       <  1:1361119/71‑1                                                                     GAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTT           >  1:516911/1‑64                                                                     GAATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAG                               >  1:986606/1‑44                                                                      AATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGT   <  1:1088109/71‑1                                                                      AATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTG                                      <  1:140103/36‑1                                                                       ATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTG  <  1:1004822/71‑1                                                                       ATTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTG  >  1:1320387/1‑71                                                                        TTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACA                                  <  1:83964/38‑1                                                                                                                                                CAATATAGCTGTAGAGTTCATCATCAACTTCAATCGTTTTCATCTTAATCCAGTGCAGTGTCTTAGCTGAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/713451‑713380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaatTATTTTTTCGCGGGTGAAACGACTCCTGG‑TTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTG  <  W3110S.gb/4125340‑4125272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.