New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 254084629 (0.870)3 (0.100) 3/88 NT 9.8% coding (1335/1728 nt) ptsI PEP‑protein phosphotransferase of PTS system
?W3110S.gb = 2540852 30 (1.020)coding (1341/1728 nt) ptsI PEP‑protein phosphotransferase of PTS system

GACGAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2540772‑2540846
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaagaaATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTTCA  <  W3110S.gb/2540852‑2540783
                                                                                                                                                 
GACGAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCA                                                                             <  1:2225878/70‑1
 ACGAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAA                                                                            <  1:2443882/70‑1
  CGAGTCAATTGAAATCGGCGTAATGGTGGCAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCA                                                                             >  1:2668671/1‑68
  CGAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAG                                                                          <  1:1608014/71‑1
   GAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGA                                                                         <  1:1056165/71‑1
                              GAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGA                                                                         <  1:2680340/44‑1
                                 ACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAA                                                                            <  1:1379052/38‑1
                                              CAACAATTGCACGTCATTTAGCCAAAGAAATCAACTTCT                                                              <  1:1224078/39‑1
                                               AACAATTGCACGTCATTTAGCCAAAGAAATCAACTTCTTT                                                            >  1:2436438/1‑40
                                                   ATTGCACGTCATTTAGCCAAAGAAATCAACTTCTT                                                             <  1:2104757/35‑1
                                                                     AAAGAAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGA       >  1:661881/1‑71
                                                                      AAGAAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCG        <  1:1287866/69‑1
                                                                      AAGAAATCAACTTCTTTGGCTAAATGACGTGCAATT                                         >  1:2438230/1‑36
                                                                        GAAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTT    <  1:1599942/71‑1
                                                                         AAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTTC   <  1:2428941/71‑1
                                                                         AAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTTC   <  1:3089557/71‑1
                                                                         AAATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTT    >  1:2363317/1‑70
                                                                          AATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCG        >  1:13618/1‑65
                                                                          AATCAACTTCTTTGGCTAAATGACGGGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTTCA  <  1:366274/71‑1
                                                                                                                                                 
GACGAGTCAATTGAAATCGGCGTAATGGTGGAAACACCGGCTGCCGCAACAATTGCACGTCATTTAGCCAAAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2540772‑2540846
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaagaaATCAACTTCTTTGGCTAAATGACGTGCAATTGTTGCGGCAGCCGGTGTTTCCACCATTACGCCGATTTCA  <  W3110S.gb/2540852‑2540783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.