New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 113112040 (1.040)15 (0.520)
+GTAGCCGGACTT		 4/74 NT 40.7% coding (288/417 nt) flgN export chaperone for FlgK and FlgL
?W3110S.gb = 4641028 18 (0.470)coding (340/690 nt) creB DNA‑binding response regulator in two‑component regulatory system with CreC

CAATCTGTCCTTCCAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1131104‑1131120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCGCACACTTCGC  <  W3110S.gb/4641028‑4641014
                 ||||||||||||               
aAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    <  1:724590/41‑1
aAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    <  1:86258/41‑1
aAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCAC         <  1:3490220/36‑1
aAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCA          >  1:2275823/2‑36
aAATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCA          <  1:2757175/35‑1
 AATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCaa  >  1:2204005/1‑41
 AATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCaa  >  1:2706848/1‑41
  ATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCaa  >  1:1013110/1‑40
  ATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCaa  >  1:601261/1‑40
  ATCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    >  1:3678339/1‑40
   TCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTCaa  >  1:1068036/1‑39
   TCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    >  1:1007620/1‑39
   TCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    >  1:2081041/1‑39
   TCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTTC    <  1:50172/39‑1
   TCTGTCCTTCCAGTGTAGCCGGACTTTGGCGCACACTT     <  1:2539455/38‑1
                 ||||||||||||               
CAATCTGTCCTTCCAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1131104‑1131120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCGCACACTTCGC  <  W3110S.gb/4641028‑4641014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.