New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 3071050 =38 (1.020)28 (0.950)
+AAATGCTCTC		 3/80 NT 48.7% coding (229/1164 nt) pgk phosphoglycerate kinase
?W3110S.gb 4347351 = 36 (0.970)coding (761/1356 nt) melA alpha‑galactosidase, NAD(P)‑binding

TTCTCTCTGCTGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3071065‑3071050
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTTCAAAAAGCT  >  W3110S.gb/4347351‑4347363
                ||||||||||             
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:1099782/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:1175938/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:103064/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:1414373/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:661070/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:1742393/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:6209/2‑37
gTCTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:454315/2‑37
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:1816510/37‑1
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:2119017/37‑1
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:3612685/37‑1
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:3426951/37‑1
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:1901507/1‑36
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:2607202/1‑36
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:2439304/1‑36
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:1634834/1‑36
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:921361/1‑35
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:3149765/1‑35
  CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:1985980/1‑35
  CTCTATGCTGCCGGAAATGCTCTCTGTTCAAAAAG    >  1:558581/1‑35
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  >  1:1313157/1‑36
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:2996287/1‑35
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:2877201/1‑35
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:2209517/1‑35
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:1641751/1‑35
   TCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGC   >  1:841159/1‑35
    CTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:2273986/35‑1
    CTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCT  <  1:2150345/35‑1
                ||||||||||             
TTCTCTCTGCTGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3071065‑3071050
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTTCAAAAAGCT  >  W3110S.gb/4347351‑4347363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.