New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 64196340 (0.820)50 (1.250)
+AGAACTGTT
5/82 NT 56.0% coding (653/1239 nt) ybdR predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
?W3110S.gb 1868457 = 56 (1.150)intergenic (‑271/‑165) mipA/yeaG scaffolding protein for murein synthesizing machinery/conserved hypothetical protein with nucleoside triphosphate hydrolase domain

CTCGGTGCGGAACAGATTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641935‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTGAAGGTGTATC  >  W3110S.gb/1868457‑1868471
                              |||||||||               
aaCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG         >  1:2401107/3‑47
aaCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG         >  1:2953948/3‑47
 aCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTG      >  1:3595499/2‑49
 aCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGT       <  1:2892136/47‑1
 aCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGT       <  1:2071070/47‑1
 aCGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:3358412/2‑45
  CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:3406317/50‑1
  CGGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTT             <  1:4227811/41‑1
   GGTGAGAAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGG        >  1:1833287/1‑45
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   <  1:2115611/43‑2
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:3761256/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:1532519/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:3352355/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:2902900/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:1831078/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:1146234/42‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:4074947/2‑37
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:1685898/2‑37
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:667108/2‑37
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGA           <  1:3904495/35‑1
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG            >  1:1103156/2‑35
         aAACAGATTTTTTGTTGTTGAAGAACTGTTTTTTG            <  1:513051/34‑1
          AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   <  1:2609137/43‑2
          AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   <  1:773669/43‑2
          AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:1145266/42‑1
          AACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGT       <  1:2171273/39‑1
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:2442347/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:4447040/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:1249401/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:4024064/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:1409307/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:3792494/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:3784017/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:1414607/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:2991827/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAga  >  1:1791081/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   <  1:704101/42‑2
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   <  1:467482/42‑2
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    >  1:93598/1‑41
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAGGT       >  1:1545019/1‑38
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAAG         >  1:1448074/1‑36
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:1238611/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:1667085/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:607083/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:1076327/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:2071847/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:842194/1‑35
           ACAGATTTTTTGTTGTTGAAGAACTGTTTTTTGAA          >  1:1557259/1‑35
                tTTTTTGTTGTTGAAGAACTGTTTTTTGATGGTGTA    <  1:595131/35‑1
                 TTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    <  1:4297624/35‑1
                 TTTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTA    >  1:1613288/1‑35
                  TTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   >  1:4087082/1‑34
                  TTTTGTTGTTGAAGAACTGTTTTTTGAAGGTGTAg   >  1:4081723/1‑34
                              |||||||||               
CTCGGTGCGGAACAGATTTTTGTTGTTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/641935‑641963
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTGAAGGTGTATC  >  W3110S.gb/1868457‑1868471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.