breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsD2C3_S161_R1_001.good.fq4,932,871313,093,777100.0%63.5 bases71 bases87.1%
total4,932,871313,093,777100.0%63.5 bases71 bases87.1%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionminE2,987,94292.414.7100.0%Escherichia coli str. K-12 substr. W3110 DNA, complete genome.
total2,987,942100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000009252
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500036
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
minE0.85792

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input02:54:59 10 Dec 201902:55:48 10 Dec 201949 seconds
Read alignment to reference genome02:55:48 10 Dec 201903:02:37 10 Dec 20196 minutes 49 seconds
Preprocessing alignments for candidate junction identification03:02:37 10 Dec 201903:03:36 10 Dec 201959 seconds
Preliminary analysis of coverage distribution03:03:36 10 Dec 201903:04:52 10 Dec 20191 minute 16 seconds
Identifying junction candidates03:04:52 10 Dec 201903:04:53 10 Dec 20191 second
Re-alignment to junction candidates03:04:53 10 Dec 201903:06:27 10 Dec 20191 minute 34 seconds
Resolving best read alignments03:06:27 10 Dec 201903:07:54 10 Dec 20191 minute 27 seconds
Creating BAM files03:07:54 10 Dec 201903:09:04 10 Dec 20191 minute 10 seconds
Tabulating error counts03:09:04 10 Dec 201903:09:27 10 Dec 201923 seconds
Re-calibrating base error rates03:09:27 10 Dec 201903:09:27 10 Dec 20190 seconds
Examining read alignment evidence03:09:27 10 Dec 201903:13:43 10 Dec 20194 minutes 16 seconds
Polymorphism statistics03:13:43 10 Dec 201903:13:44 10 Dec 20191 second
Output03:13:44 10 Dec 201903:13:54 10 Dec 201910 seconds
Total 18 minutes 55 seconds