breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome608,2141.A52.2% 23.5 / 37.4 23intergenic (‑139/+32)ydgF/dinBputative amino acid permease/nuclease inhibitor
*BS168_reference_genome2,003,2090TG50.0% 4.1 / 16.1 26intergenic (+27/+67)iseA/trnSL‑Arg1inhibitor of DL‑endopeptidases involved in cell‑separation/tRNA‑Arg
*BS168_reference_genome3,174,6800AC50.0% ‑5.8 / 10.2 32noncoding (2239/2928 nt)rrnB‑23Sribosomal RNA‑23S
*BS168_reference_genome2,003,2180GT44.4% 21.9 / 13.3 27intergenic (+36/+58)iseA/trnSL‑Arg1inhibitor of DL‑endopeptidases involved in cell‑separation/tRNA‑Arg
*BS168_reference_genome824,6920TG41.4% 21.4 / 11.2 29K9T (AAA→ACA) fecEiron‑dicitrate ABC transporter (permease)
*BS168_reference_genome2,620,9450CG41.0% 34.0 / 16.9 39L77V (CTT→GTT) yqeWputative Na+/anion cotransporter
*BS168_reference_genome3,918,0560AT37.5% 21.2 / 10.5 32V69E (GTA→GAA) qoxAcytochrome aa3‑600 quinol oxidase (subunit II)
*BS168_reference_genome4,104,0990AC35.3% 51.2 / 25.2 68L30R (CTG→CGG) yxaJputative integral membrane protein of unknown function
*BS168_reference_genome1,391,9950AC35.1% 37.7 / 10.0 37I15L (ATC→CTC) ykoGtwo‑component response regulator [YkoH]
*BS168_reference_genome2,044,4390TG34.8% 42.2 / 18.0 48S134S (TCT→TCGyoaTputative integral inner membrane protein
*BS168_reference_genome4,209,0380GT31.5% 85.9 / 12.2 54G184G (GGC→GGArsmG7‑methylguanosine methyltransferase (16S rRNA, nucleotide G527)
*BS168_reference_genome3,484,4090TA30.7% 4.1 / 23.7 101Y353F (TAT→TTT) araEarabinose‑related compounds permease
*BS168_reference_genome3,484,4040CA30.6% 2.8 / 19.1 98E355* (GAG→TAG) araEarabinose‑related compounds permease
*BS168_reference_genome966,4680TG30.6% 44.0 / 10.8 36S68R (AGC→CGC) ygaOputative integral inner membrane protein
*BS168_reference_genome608,2140GA30.0% 18.2 / 10.8 25intergenic (‑139/+32)ydgF/dinBputative amino acid permease/nuclease inhibitor
*BS168_reference_genome3,644,6810GT28.1% 116.0 / 14.0 57Q206K (CAA→AAA) degUtwo‑component response regulator
*BS168_reference_genome3,484,3650CA27.6% 6.0 / 11.6 87G368C (GGT→TGT) araEarabinose‑related compounds permease
*BS168_reference_genome4,068,1460TA27.0% 35.9 / 11.8 37*322K (TAA→AAA) frxBdesferrioxamine‑and ferrichrome‑binding transporter lipoprotein (shuttle system)
*BS168_reference_genome194,8910GC26.4% 87.8 / 12.7 53G15R (GGC→CGC) sigWRNA polymerase ECF(extracytoplasmic function)‑type sigma factor W
*BS168_reference_genome608,2191.C26.1% 45.3 / 17.4 23intergenic (‑144/+27)ydgF/dinBputative amino acid permease/nuclease inhibitor

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? BS168_reference_genome 9457 =63 (1.030)2508 (42.870) 6/242 3.7 97.2% coding (2464/2466 nt) gyrA DNA gyrase (subunit A)
?BS168_reference_genome 3484075 = 87 (1.490)coding (1392/1395 nt) araE arabinose‑related compounds permease
* ? BS168_reference_genome 3353574 =57 (0.930)3 (0.050)
+AG
3/250 5.1 5.1% coding (334/1119 nt) dadA D‑amino acid oxidase
?BS168_reference_genome = 3484371 NA (NA)coding (1096/1395 nt) araE arabinose‑related compounds permease
* ? pSIJ663_pHT315_araE_xy = 56142 (0.000)14 (0.000) 13/248 9.5 77.9% intergenic (+883/–) ampR/– –/–
?pSIJ663_pHT315_araE_xy 9532 = 6 (0.000)intergenic (+4801/–) ampR/– –/–
* ? pSIJ663_pHT315_araE_xy = 183814830 (2.090)7 (0.000) 7/250 11.7 0.060% intergenic (–/‑1140) –/eryR –/–
?pSIJ663_pHT315_araE_xy 2572 = 8634 (1.240)intergenic (–/‑406) –/eryR –/–