New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 665394	45 (0.760)	3 (0.050)	3/258	NT	6.4%	coding (920/1113 nt)	mrdB	cell wall shape‑determining protein
	?	NC_000913	= 665423	46 (0.830)					coding (891/1113 nt)	mrdB	cell wall shape‑determining protein

CAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTT  >  NC_000913/665248‑665541                                                                                                                                                   |                                                                                                                                                    cAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCgccagcc                                                                                                                                                   <  2:1012600/149‑1 (MQ=255)  aaTCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCAGACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCgccagcca                                                                                                                                                  >  2:764853/1‑149 (MQ=255)    accccAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGACCGCAACCCCAACAACCGGCAGAAAACCACTTACCAAAACAATATTTACGATGACATAACCGAATAAAAACAACATTAACCCGCCAGCAATg                                                                                                                                                >  1:603685/2‑149 (MQ=255)       cGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGAcg                                                                                                                                             >  1:510941/1‑149 (MQ=255)       cGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGCGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGAcg                                                                                                                                             >  1:28504/1‑149 (MQ=255)           cccAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGAc                                                                                                                                         <  1:2892/149‑1 (MQ=255)            ccAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGAcc                                                                                                                                        >  1:410578/1‑149 (MQ=255)            ccAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCAGCCAGCCATGACGCGAcc                                                                                                                                        >  1:877215/1‑149 (MQ=255)                      gCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGtt                                                                                                                              >  1:883413/1‑149 (MQ=255)                              aGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGagcgcaaaccacctttggtcgcgtcatggct                                                                                                                      <  2:1005823‑M1/149‑32 (MQ=255)                                 gcCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCGTACCATACCAATATTTACGAAGCCATAAACGCTTACTATCAGCATTAACCCGCCAGCCATGCGGCGACCAAAGGTGGTTTGCGCTctggc                                                                                                                   <  1:583188‑M2/149‑6 (MQ=255)                                   cGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGGAGAATACCGCTTTCCTTAGCAATTTTTACGATGACATAAACGAATAATATTAGCATTAATCCGCTAGCCATGAGGCGTCCAAAGGTGGTTTGCGCTctggagg                                                                                                                 >  2:976343‑M2/1‑142 (MQ=255)                                   cGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGagcgcaaaccacctttggtcgcgtcatggctggcgg                                                                                                                 <  2:727594‑M1/149‑37 (MQ=255)                                       cctccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTAt                                                                                                             >  1:457149/1‑149 (MQ=255)                                        ctccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACAATACCAATATTTACGAAGACAGAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATc                                                                                                            >  2:281462/1‑149 (MQ=255)                                          ccctaacTGACCCGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCGTACCATCCCAATATATACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGagcgcaaaccacctttggtcgcgtcatggctggcggcttaatg                                                                                                          <  1:921772‑M1/146‑44 (MQ=255)                                          ccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCca                                                                                                          >  1:831580/1‑149 (MQ=255)                                           cATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCcac                                                                                                         <  2:327750/149‑1 (MQ=255)                                               aCTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAACCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGcc                                                                                                     >  1:992246/1‑149 (MQ=255)                                                cTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGccc                                                                                                    >  2:629034/1‑149 (MQ=255)                                                    ccAGTGGGAGCTGAACCCCTACAACCGTCAGAATAACGCTTAACATACCAATTATTACGAAGACTTAAACGAATAATCTCAGCATTAAGCCGCCTGCCATGTCGCGACCAACTGTGGTTTGCGCTCTGGCGGCTATCCTGAGCCCgcgc                                                                                                <  2:603685/149‑1 (MQ=255)                                                         tggAGCGGAACCCCTACAAACGGCAGAATACCGCTTACAATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCGAGCAATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGAt                                                                                           <  1:62261/148‑1 (MQ=255)                                                          ggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                          <  2:363035/149‑1 (MQ=255)                                                          ggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                          <  2:729182/149‑1 (MQ=255)                                                           gAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTATGCGCTCTGGCGGCTATCGACAGCCCGCGCATGAAca                                                                                         >  2:569591/1‑149 (MQ=255)                                                                gAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                    <  2:333748/149‑1 (MQ=255)                                                                gAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGAGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                    >  2:519950/1‑149 (MQ=255)                                                                  aCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATACACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAt                                                                                  <  2:929212/149‑1 (MQ=255)                                                                  aCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAt                                                                                  <  2:410578/149‑1 (MQ=255)                                                                       tACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTaga                                                                             >  1:277187/1‑149 (MQ=255)                                                                        aCAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTagag                                                                            <  1:39438/149‑1 (MQ=255)                                                                              ggCAGAATACCGCTTACCATACCAATATTTAAGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGATTTGCGCGCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcgag                                                                      <  2:485538/149‑1 (MQ=255)                                                                               gCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcgagc                                                                     <  2:28504/149‑1 (MQ=255)                                                                               gCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAGGTAGAgagcgagc                                                                     >  1:131201/1‑149 (MQ=255)                                                                                     tcccGCATACCATACCAATATTAAGGAAGACATAAAAGAATCATATCAGCATTAACCCGCCAGCCATGACGCGACCAACGTGGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGCATGTAGAGAGCGAGcagaatc                                                               <  2:560923/147‑1 (MQ=255)                                                                                     tACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatc                                                               >  2:87983/1‑149 (MQ=255)                                                                                            taccataccaATATTTACGAATACATAAAGGCATAATATCAGCATTATGCCGCCAGCCATGACTCTACCAAAGGTGGTTTGCTCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCTGAATGTAGAGAGCGAGCAGAATCAGAATGc                                                        <  1:718132/149‑1 (MQ=255)                                                                                             accataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCAAGCCATGAGGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATccc                                                       <  1:32478/149‑1 (MQ=255)                                                                                               cataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACAAAAGGTCGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCa                                                     >  2:650481/1‑149 (MQ=255)                                                                                                ataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCAc                                                    >  2:278157/1‑149 (MQ=255)                                                                                                ataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCAc                                                    >  1:241169/1‑149 (MQ=255)                                                                                                          ttACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGc                                          <  2:510941/149‑1 (MQ=255)                                                                                                               aaGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTc                                     >  2:862491/1‑149 (MQ=255)                                                                                                                  aCATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGT‑‑AGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCa                                <  1:629034/149‑1 (MQ=255)                                                                                                                      aaaCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGt                              >  1:1022083/1‑149 (MQ=255)                                                                                                                        aCGAATAATATCAGGTTTCAGCCGACAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCTGAATGCCCTCTTATCCCAGCTCTTCCGCCAGTAc                            <  1:118493/149‑1 (MQ=255)                                                                                                                        aCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCTGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAc                            >  1:825209/1‑149 (MQ=255)                                                                                                                        aCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAc                            >  1:816829/1‑149 (MQ=255)                                                                                                                          gAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAccc                          >  2:89921/1‑148 (MQ=255)                                                                                                                           aatactATCAGCATTACGCCGCCAGCCATGACGCGACCAAAGGTGGGTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGGTCTTCCGCCAGTACcgc                         <  2:216662/149‑1 (MQ=255)                                                                                                                             taataTCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCCGCTCTTCCGCCAGTACCGCGa                       >  2:206320/1‑149 (MQ=255)                                                                                                                                     gCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCCCAGCCCGCGCATGATCCGCAGAATGTAGAGAGCGCGCAGAATCAGAATGCCCACTACTCCCAGCTCGTCCGCCAGTCCCGCGGTGATagag               >  1:859036/1‑149 (MQ=255)                                                                                                                                      cATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtatatattgggatggtacgcggtattctgccggttgtaggggttccgctcccacgggtcagttaggg              <  1:921772‑M2/149‑107 (MQ=255)                                                                                                                                             cctccagagcgcaaaccacctttggacgcctcatggctagcggattaatgctaatattattcgtttatgtcatcgtaaaaattgctaaggaaagcggtattctcccggttgtaggggttccgctcccactggtcagttatggaggatcg       >  2:976343‑M1/4‑7 (MQ=255)                                                                                                                                             ccgccAGCCATGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcg       <  2:727594‑M2/149‑114 (MQ=255)                                                                                                                                              cgccAGCCCTGACGCGACCAAAGGTGGTTTGAGCTCTGGCGGCTATCCACCGCCCGCGCATGATCCGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCACAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCCGGATgg      >  1:721543/1‑149 (MQ=255)                                                                                                                                               gccAGagcgcaaaccacctttggtcgccgcatggctggcgggttaatgctgatagtaagcgtttatggcttcgtaaatattggtatggtacgcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcggc     <  1:583188‑M1/149‑145 (MQ=255)                                                                                                                                                  agccaTGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcggcgct  <  2:1005823‑M2/149‑119 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CAATCCCGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTT  >  NC_000913/665248‑665541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.