New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 665394	45 (0.760)	3 (0.050)	3/258	NT	6.4%	coding (920/1113 nt)	mrdB	cell wall shape‑determining protein
	?	NC_000913	= 665423	46 (0.830)					coding (891/1113 nt)	mrdB	cell wall shape‑determining protein

GCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGC  >  NC_000913/665280‑665571                                                                                                                                                |                                                                                                                                                     gcCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCGTACCATACCAATATTTACGAAGCCATAAACGCTTACTATCAGCATTAACCCGCCAGCCATGCGGCGACCAAAGGTGGTTTGCGCTctggc                                                                                                                                                 <  1:583188‑M2/149‑6 (MQ=255)   cGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGGAGAATACCGCTTTCCTTAGCAATTTTTACGATGACATAAACGAATAATATTAGCATTAATCCGCTAGCCATGAGGCGTCCAAAGGTGGTTTGCGCTctggagg                                                                                                                                               >  2:976343‑M2/1‑142 (MQ=255)       cctccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTAt                                                                                                                                           >  1:457149/1‑149 (MQ=255)        ctccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACAATACCAATATTTACGAAGACAGAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATc                                                                                                                                          >  2:281462/1‑149 (MQ=255)          ccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCca                                                                                                                                        >  1:831580/1‑149 (MQ=255)           cATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCcac                                                                                                                                       <  2:327750/149‑1 (MQ=255)               aCTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAACCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGcc                                                                                                                                   >  1:992246/1‑149 (MQ=255)                cTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGccc                                                                                                                                  >  2:629034/1‑149 (MQ=255)                    ccAGTGGGAGCTGAACCCCTACAACCGTCAGAATAACGCTTAACATACCAATTATTACGAAGACTTAAACGAATAATCTCAGCATTAAGCCGCCTGCCATGTCGCGACCAACTGTGGTTTGCGCTCTGGCGGCTATCCTGAGCCCgcgc                                                                                                                              <  2:603685/149‑1 (MQ=255)                         tggAGCGGAACCCCTACAAACGGCAGAATACCGCTTACAATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCGAGCAATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGAt                                                                                                                         <  1:62261/148‑1 (MQ=255)                          ggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                                                        <  2:729182/149‑1 (MQ=255)                          ggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                                                        <  2:363035/149‑1 (MQ=255)                           gAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTATGCGCTCTGGCGGCTATCGACAGCCCGCGCATGAAca                                                                                                                       >  2:569591/1‑149 (MQ=255)                                gAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                                                  <  2:333748/149‑1 (MQ=255)                                gAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGAGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                                                  >  2:519950/1‑149 (MQ=255)                                  aCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATACACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAt                                                                                                                <  2:929212/149‑1 (MQ=255)                                  aCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAt                                                                                                                <  2:410578/149‑1 (MQ=255)                                       tACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTaga                                                                                                           >  1:277187/1‑149 (MQ=255)                                        aCAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTagag                                                                                                          <  1:39438/149‑1 (MQ=255)                                              ggCAGAATACCGCTTACCATACCAATATTTAAGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGATTTGCGCGCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcgag                                                                                                    <  2:485538/149‑1 (MQ=255)                                               gCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcgagc                                                                                                   <  2:28504/149‑1 (MQ=255)                                               gCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAGGTAGAgagcgagc                                                                                                   >  1:131201/1‑149 (MQ=255)                                                     tcccGCATACCATACCAATATTAAGGAAGACATAAAAGAATCATATCAGCATTAACCCGCCAGCCATGACGCGACCAACGTGGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGCATGTAGAGAGCGAGcagaatc                                                                                             <  2:560923/147‑1 (MQ=255)                                                     tACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatc                                                                                             >  2:87983/1‑149 (MQ=255)                                                            taccataccaATATTTACGAATACATAAAGGCATAATATCAGCATTATGCCGCCAGCCATGACTCTACCAAAGGTGGTTTGCTCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCTGAATGTAGAGAGCGAGCAGAATCAGAATGc                                                                                      <  1:718132/149‑1 (MQ=255)                                                             accataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCAAGCCATGAGGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATccc                                                                                     <  1:32478/149‑1 (MQ=255)                                                               cataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACAAAAGGTCGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCa                                                                                   >  2:650481/1‑149 (MQ=255)                                                                ataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCAc                                                                                  >  1:241169/1‑149 (MQ=255)                                                                ataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCAc                                                                                  >  2:278157/1‑149 (MQ=255)                                                                          ttACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGc                                                                        <  2:510941/149‑1 (MQ=255)                                                                               aaGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTc                                                                   >  2:862491/1‑149 (MQ=255)                                                                                  aCATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGT‑‑AGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCa                                                              <  1:629034/149‑1 (MQ=255)                                                                                      aaaCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGt                                                            >  1:1022083/1‑149 (MQ=255)                                                                                        aCGAATAATATCAGGTTTCAGCCGACAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCTGAATGCCCTCTTATCCCAGCTCTTCCGCCAGTAc                                                          <  1:118493/149‑1 (MQ=255)                                                                                        aCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCTGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAc                                                          >  1:825209/1‑149 (MQ=255)                                                                                        aCGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAc                                                          >  1:816829/1‑149 (MQ=255)                                                                                          gAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAccc                                                        >  2:89921/1‑148 (MQ=255)                                                                                           aatactATCAGCATTACGCCGCCAGCCATGACGCGACCAAAGGTGGGTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGGTCTTCCGCCAGTACcgc                                                       <  2:216662/149‑1 (MQ=255)                                                                                             taataTCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCCGCTCTTCCGCCAGTACCGCGa                                                     >  2:206320/1‑149 (MQ=255)                                                                                                     gCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCCCAGCCCGCGCATGATCCGCAGAATGTAGAGAGCGCGCAGAATCAGAATGCCCACTACTCCCAGCTCGTCCGCCAGTCCCGCGGTGATagag                                             >  1:859036/1‑149 (MQ=255)                                                                                                      cATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtatatattgggatggtacgcggtattctgccggttgtaggggttccgctcccacgggtcagttaggg                                            <  1:921772‑M2/149‑107 (MQ=255)                                                                                                             ccgccAGCCATGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcg                                     <  2:727594‑M2/149‑114 (MQ=255)                                                                                                              cgccAGCCCTGACGCGACCAAAGGTGGTTTGAGCTCTGGCGGCTATCCACCGCCCGCGCATGATCCGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCACAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCCGGATgg                                    >  1:721543/1‑149 (MQ=255)                                                                                                                  agccaTGACGCGACCAAAGGTGGTTTGCGCTctggcggcttaatgctgatattattcgtttatgtcttcgtaaatattggtatggtaagcggtattctgccggttgtaggggttccgctcccactggtcagttatggaggatcggcgct                                <  2:1005823‑M2/149‑119 (MQ=255)                                                                                                                      aTGAAGCGCCCAAAGGCGGGTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCACAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCggg                            <  2:583188/149‑1 (MQ=255)                                                                                                                         aCGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGgag                         <  1:281462/149‑1 (MQ=255)                                                                                                                           gcgACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCGCGCTCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGaa                       <  2:992246/149‑1 (MQ=255)                                                                                                                              aCCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGAAGAATGGAGAGAGCGAGCAGAATCAGAATGCCCACTAATCACCGCTCTTCCGCCAGTACCCCGAAGATAAAGTCAGTATGGCGTTCTGGGAGAAAtt                    >  1:641314/1‑149 (MQ=255)                                                                                                                               ccAAAGGTGGTTTGCGCTCTGGCGGCTATACACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTc                   <  1:764853/149‑1 (MQ=255)                                                                                                                                 aaaGGTGGTTTGCGCTCTGGCGGCTATCCACAGACCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGATGATATAGTCAGTATGGCGTTCGGGGAGAATTTCaa                 >  1:98570/1‑149 (MQ=255)                                                                                                                                  aaGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGGTCGGGGAGAAATTCAAg                >  1:373601/1‑149 (MQ=255)                                                                                                                                   aGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGc               >  2:58578/1‑149 (MQ=255)                                                                                                                                         tttGCGCTCTGGCGGCTTTACACAGCCCGCGCATGATCAGCAGAATGTAGCGCGCGAGCAGAATCAGAATCCCCACTAATCCCAGCTCTTCCGCCAGTCCCTCGAAGCTAAAGTCATTCTGGCGATCGGGGAGAAATTCAAGCTGAGAc         >  2:666377/1‑149 (MQ=255)                                                                                                                                          ttGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACt        >  2:853801/1‑149 (MQ=255)                                                                                                                                            gcgcTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGa      <  1:87983/149‑1 (MQ=255)                                                                                                                                               ctctGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGTGAGAAATTCAAGCTGTGACTGAGTg   >  2:18730/1‑149 (MQ=255)                                                                                                                                                tctGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAAGGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGGACCTCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGc  <  2:816981/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGC  >  NC_000913/665280‑665571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.