Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	3,190,486	IS5 (+) +4 bp	24.1%	coding (606‑609/750 nt)	yqiH →	putative fimbrial chaperone YqiH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	6 (0.140)	6/264	NT	20.9%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3190486 =	22 (0.540)					coding (606/750 nt)	yqiH	putative fimbrial chaperone YqiH
*	?	NC_000913	3130146 =	NA (NA)	8 (0.190)	8/262	NT	26.1%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3190489	22 (0.540)					coding (609/750 nt)	yqiH	putative fimbrial chaperone YqiH

GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289057‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTA  >  NC_000913/3190486‑3190601                                                                                                                                                                                                                                                                 GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAT                                                                                                                  <  1:533587/143‑1                     AAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTAAAAA                                                                                              <  1:365216/143‑1                                            TTCAAGGCAGTTACGGGAGAAAAAATCGGCTCAAACATGAAGAGATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGG                                                                       <  1:19317/143‑1                                                                         CTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATTACTTTGGTGGATTAAGT                                          >  2:287249/1‑143                                                                                  GAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTAAAACTGTTCCGTCACCTTTTCAGGTGGGTTATATCACTGACTGTGGTGGATTAAGTTTGTATGAA                                 >  1:24495/1‑143                                                                                         TGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACT                          <  1:481852/143‑1                                                                                                                 GAATTTCCCCGCTTATTCGCACCTTCCCTATGCAAATACGCAGTTTACAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTA  >  1:208972/1‑143                                                                                                                                                                                                                                                                 GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289057‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTA  >  NC_000913/3190486‑3190601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.