Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	45 (1.090)	39/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	36 (0.870)	33/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC  >  NC_000913/2101749‑2101889                                                                                                                                                                                                                                                                                             AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGG                                                                                                                                              >  1:465514/1‑143       TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAGATAGTGTACTGATTCTCCGCATTAAACAATTGGTTAATATATGTAAAACATTTCATTAAAATTACGTTGATTGTTGCCTTTCTTTGTTTTTGATTTGGGAGGGT                                                                                                                                        >  1:424808/1‑143       TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTAGCTAATTAGGGAAGGT                                                                                                                                        >  2:3924/1‑143                   TATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGG                                                                                                                            <  1:563621/143‑1                    ATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAATATTTCATTAACATGAAGTTGATTGTTGCCTTTCTGTGTATTTAATTAGGGAAGGTGCGAATAAGCGGG                                                                                                                           <  2:118854/143‑1                    ATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGG                                                                                                                           >  1:604471/1‑143                     TAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGG                                                                                                                          >  1:250659/1‑143                        TTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAA                                                                                                                       <  1:446819/143‑1                          TCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACCTTAAGTTGATTGTTGCCTTTCTTTGTATTTACTTAGGGAAGGTGCGTATAAGCGGGGAAATT                                                                                                                     >  2:178940/1‑143                                 TAATTATAAGAAAATAGTTTTATGATTCTCTGCATTAAACAATTGGTTAATATATTTAGAACATTTCATTAACATTACGTTGATAGTTTCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCG                                                                                                              <  2:313619/143‑1                                  AATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTCCTCGG                                                                                                             <  2:677362/143‑1                                             AATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTC                                                                                                  <  1:72273/143‑1                                             AATAGTGTACTAATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCAGTC                                                                                                  >  2:363936/1‑143                                               TAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCAGTCAT                                                                                                >  1:146884/1‑143                                                  TGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAATTTCTTCTCGGCTGACTCAGTCATTTC                                                                                             >  1:158078/1‑143                                                     ACTGATTCTCCGCCTTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTTCCTGTCGTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATT                                                                                          <  2:738835/143‑1                                                         ATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTT                                                                                      >  2:208269/1‑143                                                              CCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTAAGTATTTAATTAGGGAAGGTGCTAATAAGCGGGGCTATGCTACTCGCCTGACTCAGTCATGTCATTTCGCCATGT                                                                                 >  2:573909/1‑143                                                                  ATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                             <  2:744145/143‑1                                                                   TTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAG                                                                            <  1:24974/143‑1                                                                   TTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAG                                                                            <  1:682703/143‑1                                                                         AAGTGGTTAATATATGTAAAACATTTCATTAACATTCAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGGAGAAGCGGGGAAATGCTTGTCGGCTGACTCAGTCATTTAATGTCTCCATGGTTGAGCCGgct                                                                      >  2:697809/1‑140                                                                              GTTAAGATATGTATAACTTTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTC                                                                 <  2:78000/143‑1                                                                                  ATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCC                                                             <  1:192244/143‑1                                                                                  ATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCC                                                             <  1:506244/143‑1                                                                                  ATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTGCTTCATGTTTGCGCCGCTTCTTTCTCCC                                                             >  2:523399/1‑143                                                                                       TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAA                                                        <  2:154756/143‑1                                                                                        GTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAAT                                                       >  2:773572/1‑143                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                      >  1:257488/1‑143                                                                                           AAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTACGCCGATTTTTTCTCCCGTAAATTCC                                                    >  2:434637/1‑143                                                                                             ACATTTCATTAAACTTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTT                                                  <  2:753144/143‑1                                                                                               atcTCATTGACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATCAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGA                                                <  2:817935/140‑1                                                                                                TTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                               <  1:337668/143‑1                                                                                                    ATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATCTCATTCCTTCATGTTTGAGCCGCTTTCTTCTCCCGTCAATCCCTTCAATCga                                           >  2:242380/1‑141                                                                                                            TAAGTTGATTGTTGCTTTTCTTTGTATTTAATTAGGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTATTTCTCCCGTAAATGCCTTGAATCAGCCTATTTA                                   <  2:449677/143‑1                                                                                                                          CCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                     >  1:349666/1‑143                                                                                                                          CCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                     <  2:498013/143‑1                                                                                                                            TTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCA                   >  2:5289/1‑143                                                                                                                              TCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATT                 <  1:266160/143‑1                                                                                                                                TTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTTACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA               >  1:633263/1‑143                                                                                                                                TTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTTACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAGTGGCTTTAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA               >  1:633283/1‑143                                                                                                                                   GTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGG            <  1:3924/143‑1                                                                                                                                    tggtttcTTACGGAAGGCGCGAATAAGCGGGGAAATTCGTCTCGGCTGACTCAGTCATTTCATTGCTTCATGTTCGAGCCGCTCTTTTCTCCCGTAAATGCCTTGAATCAGCCTATCTAGACCGTTTCTTCGCCATTTAAGGC           <  2:32412/136‑1                                                                                                                                    TATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCATTTAAGGC           >  2:242872/1‑143                                                                                                                                    TATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAGCGTTTCTTCGCAATGTAAGGC           >  1:504450/1‑143                                                                                                                                      TTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTACAGCGATTCTTCGCCATTTAAGGCGT         >  2:387060/1‑143                                                                                                                                         AATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT      >  1:617608/1‑143                                                                                                                                           TTAGGGAAGTTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATCCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC    <  1:82342/143‑1                                                                                                                                           TTAGGGAAGGTGCGATTAAGCCGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACTGTTTCTTCGCCATTTAAGTCGTTATCC    <  2:229868/143‑1                                                                                                                                           TTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAGTTCGCCTGGTTCGACCGTTTCTTCGCCATTTAAGGCGTTATCg    >  1:148303/1‑142                                                                                                                                            TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCATTTAATGCGTTATCCC   >  1:574813/1‑143                                                                                                                                            TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC   <  2:640581/143‑1                                                                                                                                             AGGGAAGGTGCGAGTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAATTAGCCCGTGTCTTCGCCATTTAAGGCGTTATCCCC  <  2:329716/143‑1                                                                                                                                                                                                                                                                                             AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC  >  NC_000913/2101749‑2101889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.