Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	45 (1.090)	39/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	36 (0.870)	33/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289981‑2290255                                                                                                                                     |                                                                                                                                               tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                      <  1:558228/143‑1 (MQ=38)       cTGGCCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                <  1:49721/143‑1 (MQ=255)          gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAg                                                                                                                             >  1:494713/1‑143 (MQ=255)            cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGaa                                                                                                                           <  1:211654/143‑1 (MQ=255)                    gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGGTGACAAACATGATCTCATACCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAGAtt                                                                                                                   >  1:120753/1‑143 (MQ=255)                     cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTg                                                                                                                  <  1:700148/143‑1 (MQ=255)                     cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTg                                                                                                                  <  1:634200/143‑1 (MQ=255)                      tcaaggctgAAATCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                                 <  1:298034/138‑1 (MQ=255)                          tgctgTATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTGCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                             >  2:676660/1‑143 (MQ=255)                          tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                             >  1:554900/1‑143 (MQ=255)                           gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATCTTGCGGAAc                                                                                                            >  2:604161/1‑143 (MQ=255)                                aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaa                                                                                                       >  1:799405/1‑143 (MQ=255)                                 ttCACTGTCGGCGAAGGTAAGTTGATAACTCATGATGACCCCTGCTCGAGGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                      <  1:111004/143‑1 (MQ=18)                                       gTCGGCGTGGGTCAGGTGATGATTCATTATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCGCATATCAGGGATTTGTTTGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCTGAACGACAAAGAGaaa                                                                                                <  2:246184/143‑1 (MQ=21)                                        tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAAc                                                                                               >  2:744510/1‑143 (MQ=255)                                           gCGAAGGTAAGTTGATGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGGACGACAAAGAGAAAAAGa                                                                                            >  1:316660/1‑143 (MQ=255)                                              acggTAAGTCGATTACGCAGGATGAACCCTGTTCTATGGCCCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAcc                                                                                         <  2:784580/141‑1 (MQ=21)                                                   aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGCGACTTGTTCGCACCTACCACAACGCTCTCGCCACCAGAACAGATATCGCCGCACGACCACGAGAAACAGAAcccaaca                                                                                    >  2:797500/1‑138 (MQ=17)                                                           gaCTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                            >  2:366181/1‑143 (MQ=255)                                                           gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAGCGACAAAGAGAAACAGAAACAGATTGATGCagt                                                                            >  2:752405/1‑141 (MQ=255)                                                             cTCACGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGa                                                                          <  2:805936/143‑1 (MQ=255)                                                                 tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGCGAAACAGAACCAGATTGATGCATTGAGCtt                                                                      >  1:147977/1‑143 (MQ=255)                                                                  gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGAATTGTTCGCAAATTCCACAACCATGCCCACACCAGCCCCGCCATAGCGGACCGACCCAGAGAAACAGAACCAGATTGATGCATTGAGCttt                                                                     >  1:138361/1‑143 (MQ=12)                                                                   atgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTc                                                                    <  1:438873/143‑1 (MQ=255)                                                                         cccgTTCTATGGCACCAGATGACAGACATGATCTCATATCAGGGACGTGTGCGCACCTTCCATAACGCTGTAGCCACCATAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              <  1:766333/140‑1 (MQ=38)                                                                         ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGCTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  2:369640/1‑143 (MQ=255)                                                                         ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCACGAGAAAGAGAACCAGCACCAGATTGATGCCTTGAGCTTTCATCCTa                                                              >  2:390398/1‑143 (MQ=37)                                                                         ccTGTGCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCAGCAGAAGAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCGTTGGGGtttgaggaga                                                              >  2:115074/1‑136 (MQ=25)                                                                                 aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGACACCAGAACAGATATTGCGGGAGGTCACAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATGCTATGAaatta                                                      >  1:184115/1‑143 (MQ=37)                                                                                  tGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAGCCAGATTGATGCATTGAGCTTTCATCCTATGAaattaa                                                     >  1:124942/1‑143 (MQ=255)                                                                                                        tctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTa                               >  1:474542/1‑143 (MQ=255)                                                                                                            atatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAATTAACAGTCATCCTATGAAATTAATTGCTTTTAAAAGCATTGGGTACAGa                           >  2:577687/1‑143 (MQ=255)                                                                                                                cAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACAGATATTGCGGAACGACAAAGAGAAACAGACCCAGGTTGATGCATTCAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTTGGTCCAGAAAAt                       >  1:600531/1‑143 (MQ=255)                                                                                                                cAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAGCCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAAt                       <  2:124942/143‑1 (MQ=255)                                                                                                                    gACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTACTGTTAACAGCATTGGGTACAGAAAATAccc                   <  2:100114/143‑1 (MQ=255)                                                                                                                            ggcctcTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGTACGCAAAACAGAAACAGAACCAGATTGAGGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTcc           <  2:132165/138‑1 (MQ=255)                                                                                                                             gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGGGTCATAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGccaca          >  2:362380/1‑139 (MQ=255)                                                                                                                             gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTACa          >  2:342070/1‑143 (MQ=255)                                                                                                                                     cATAACGCTGTAGCCACCAGAACAGATCTTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAACTTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAACGTCATACCCggc  >  2:72060/1‑142 (MQ=255)                                                                                                                                     |                                                                                                                                               TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289981‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.