New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 427762	50 (1.210)	3 (0.080)	3/250	NT	6.1%	coding (116/1848 nt)	secD	Sec translocon accessory complex subunit SecD
	?	NC_000913	= 427779	45 (1.150)					coding (133/1848 nt)	secD	Sec translocon accessory complex subunit SecD

GCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAAGCATTAAT  >  NC_000913/427644‑427921                                                                                                                                        |                                                                                                                                               gCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCACCCTGCTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGCGTCGCCGccagtgc                                                                                                                                         <  2:114080‑M2/143‑8 (MQ=255)        tAAACTGTTATGCTTTGTGGAAGTACGTCATGCTGATCGTGGTGGTTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACg                                                                                                                                  <  2:83097/143‑1 (MQ=255)            ccGTTATCCTGCGAGTAAGTACGTCATGCTGATCGTGGTGAGTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGa                                                                                                                              <  1:360852/143‑1 (MQ=255)              gTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTTGTGAGGATCCGGCTGTTCATATCTCTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATc                                                                                                                            <  1:200258/143‑1 (MQ=255)                     tttGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTcc                                                                                                                     >  1:129620/1‑143 (MQ=255)                      ttGCGGAAGTACGTCCTGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCa                                                                                                                    <  1:183195/143‑1 (MQ=255)                       tgtgGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCATCCTGTTTGGTGAGGCTCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAg                                                                                                                   <  2:204424/143‑1 (MQ=255)                           gAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGaaaa                                                                                                               <  1:340626/143‑1 (MQ=255)                              gtccGTCATGCGGAGCGTGGTGATTGTCGGCGGTCTGCTGCGTGCGCTTCCCGACCTGTGTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGGCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGt                                                                                                            <  2:146625/140‑1 (MQ=255)                               tACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGtt                                                                                                           >  2:314033/1‑143 (MQ=255)                                      tgcgcttCGTGGTGTTTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACaagaa                                                                                                    <  2:305375/137‑1 (MQ=255)                                      tGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACaagaa                                                                                                    <  2:209077/143‑1 (MQ=255)                                             cGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGaaaaaa                                                                                             <  1:426264/143‑1 (MQ=255)                                                ggtgATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATaa                                                                                          <  1:314033/143‑1 (MQ=255)                                                   gATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGCTCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAACCGTCACAAGACGAAAAAATAACTg                                                                                       >  2:379260/1‑143 (MQ=255)                                                       gTCATGGGTCTGCTGTATGCGCTTCCCAACCGGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTaa                                                                                   <  2:389310/143‑1 (MQ=255)                                                        tCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTACTAAg                                                                                  >  2:223790/1‑143 (MQ=255)                                                          aTCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGCTACACGAAGCCAAAATCACTCCTCAGTc                                                                                >  1:475689/1‑143 (MQ=255)                                                            cGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTa                                                                                    <  2:633482/137‑1 (MQ=255)                                                            cGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTa                                                                                    >  1:633482/1‑137 (MQ=255)                                                                   ctgTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAATAAGAAAAAATAACTGCTAAGTCTGTGGCACt                                                                       <  1:346278/143‑1 (MQ=255)                                                                   ctgTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACt                                                                       >  1:198541/1‑143 (MQ=255)                                                                   ctgTATGCGATTCCCAACGTGTTTGATGAGGATGCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGCATGAAGTTTGTTTCAAGTGCAGATAACGTTACAAGAAAAAAAAAGAAGTAGTAAGTCTAAGGCagt                                                                       >  2:360907/1‑141 (MQ=255)                                                                        tGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGCAAACGTCACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAag                                                                  >  2:530507/1‑143 (MQ=255)                                                                           gcTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGCgg                                                               >  1:353712/1‑143 (MQ=255)                                                                                 cAACCTGTTTGGTGAGGATCCGGCTGTCCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTa                                                         <  1:137280/143‑1 (MQ=255)                                                                                  aaCCTGTTGGGTGAGGAACCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTAt                                                        <  1:564486/143‑1 (MQ=255)                                                                                        tttGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGCCACTGGAAGAGGGCGCTATTCTTgc                                                  >  1:754033/1‑143 (MQ=255)                                                                                        tttGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACAGCTAAGTCGGTGGCACTGGAAGAGGGGGCCATTCGTgc                                                  >  2:368192/1‑143 (MQ=255)                                                                                             tGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGACAACGTTGGGAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCt                                             >  1:810567/1‑143 (MQ=255)                                                                                              gAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGCGGCACAGGAATATGTCGCTATTCTGGAGCGCtc                                            >  2:319770/1‑142 (MQ=255)                                                                                               aGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCGAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTGTGTGGCACTGGAAGCGGGAGGTACTCTTGCGCGCTTc                                           >  2:427380/1‑143 (MQ=255)                                                                                                 gATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGGTAAGTCTGAGGCCCTGGAAGCGGGCGCTATTCTTGCGCGCTTCGa                                         >  2:604747/1‑143 (MQ=255)                                                                                                  aTCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGAc                                        <  1:657365/143‑1 (MQ=255)                                                                                                     cGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTcc                                     >  2:410582/1‑143 (MQ=255)                                                                                                          gTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGa                                <  1:84691/143‑1 (MQ=255)                                                                                                          gTTCAGATCACTGGGGCGCCCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGTGCGCTATTCTTGCGCGCTTCGACTCCACTGa                                <  1:288532/143‑1 (MQ=255)                                                                                                          gTTCACATCACTGGGGCGCGCGGCCTCGCCGCCAGTGAGCAAACGCTGAGCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGa                                <  2:686319/143‑1 (MQ=255)                                                                                                            tCAGATCACTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatgacgtacttccacaaaggataa                                       >  1:679203‑M2/1‑29 (MQ=255)                                                                                                            tCAGATCACTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggttgggaagcgcatacagcagaccgatgacaatcaccacgatcagcatgacgtacttccacaaaggataa                                       <  2:679203‑M2/134‑106 (MQ=255)                                                                                                             cAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGacac                             >  1:798135/1‑143 (MQ=255)                                                                                                              aGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACAcc                            >  1:630111/1‑143 (MQ=255)                                                                                                              aGATCACTGGTGCGCGCGGAGGCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACCAGAAGAAACAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCGCTTCTTGCGCGCTTCGGCTCGAcgtgcacc                            >  2:586531/1‑136 (MQ=255)                                                                                                                aTCACTGGTGCGCGCGGAGTCGCCGccagtgatctgaacagccggatcctcaccaaacaggtggggaagcgcatacagcagaccgatgacaatcaccacgatcagcatgacgtacttccacaaaggataacgctttaacacgc                          <  2:175608‑M2/143‑119 (MQ=255)                                                                                                                aTCACTGGTGCGCGCGGAGTCGCCGCCAGTAAGCAAACGCTGATCCAGGTTCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGAAAGAGGGCGCTGTTCTTGCGACCTTCGGCTCCACTGACACCCa                          >  1:808112/1‑143 (MQ=255)                                                                                                                  cACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAACAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGt                        >  1:194366/1‑143 (MQ=255)                                                                                                                   aCTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGtt                       <  2:324415/143‑1 (MQ=255)                                                                                                                             gcgGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGt             <  1:114080/143‑1 (MQ=255)                                                                                                                                  gTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGATAACGTTACAAGAAGAAAAAATAACCTCTATGAATGTTGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCCCTGACACCCAGCTCGGCGCTCGTgcacc        >  1:289334/1‑139 (MQ=255)                                                                                                                                     gccgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAAGAAtt     >  1:125066/1‑143 (MQ=255)                                                                                                                                       cgccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAAGCATTaa   <  1:649297/143‑1 (MQ=255)                                                                                                                                        gccAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAAGCATTAAt  >  1:369418/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                               GCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTCGCCGCCAGTGAGCAAACGCTGATCCAGGTCCAGAAAACGTTACAAGAAGAAAAAATAACTGCTAAGTCTGTGGCACTGGAAGAGGGCGCTATTCTTGCGCGCTTCGACTCCACTGACACCCAGTTGCGCGCTCGTGAAGCATTAAT  >  NC_000913/427644‑427921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.