New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4162839	52 (1.260)	3 (0.080)	3/250	NT	5.8%	coding (432/1101 nt)	trmA	tRNA m(5)U54 methyltransferase
	?	NC_000913	= 4162849	49 (1.250)					coding (422/1101 nt)	trmA	tRNA m(5)U54 methyltransferase

AAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACC  >  NC_000913/4162707‑4162991                                                                                                                                               |                                                                                                                                               agaGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTACTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCAt                                                                                                                                                <  2:709988/141‑1 (MQ=255)        ttttCTACCTGCCGGTAGATCATCTCTTTCCCTGCGACCGGCACCCGTTCTTCGATGTAATCTTTATCCTGCTCGATTTTGCTTTTCGTTTCCCGACCAATCATATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcag                                                                                                                                         >  1:303211‑M2/1‑136 (MQ=255)           tCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATGGCGCAGGGc                                                                                                                                      >  1:28967/1‑143 (MQ=255)             gacgtGAAGGGAGATCGTCGCTTGGGCTGCGACCGGCAGAGGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGGTTTCGTTGGCCGAACAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCt                                                                                                                                    <  1:364897/139‑1 (MQ=255)              aCCGGACGGTACATCCTCGCTGTCCCTGCGACTGCCAGACGTTCATCGCTGTAATCCTGATCCAGCTCGAGTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATCCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTc                                                                                                                                   <  2:221041/143‑1 (MQ=255)                    ccgTAGCTCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATCCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTc                                                                                                                             <  2:468525/141‑1 (MQ=255)                       tAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTg                                                                                                                          >  1:41529/1‑143 (MQ=255)                         gttcatcTCTTTCCCTGCGACCGGCCGCCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGAc                                                                                                                        <  2:626533/141‑1 (MQ=255)                            catcTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGcc                                                                                                                     >  2:448684/1‑143 (MQ=255)                             atcTCTTTCCCTGCGACCGGAAGACGATAATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGCTGCACATTCAGATTCTGAGCGAGCCGTACAACGCGCAGAGCCGCCGCTACCTGAccggg                                                                                                                    >  2:402278/1‑139 (MQ=255)                                tctTTCCCTGCGACCGGCAGACGGTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGCCCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGgcgcgccgc                                                                                                                 >  1:309626/1‑138 (MQ=255)                                 ctTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtca                                                                                                                <  1:500038/143‑1 (MQ=255)                                  tttCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACtcat                                                                                                               <  1:238686/143‑1 (MQ=255)                                      ccTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATCTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCatcatc                                                                                                           <  1:52626/143‑1 (MQ=255)                                      ccTGCGACCGGCAGACGTTCAGCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCatcatc                                                                                                           <  1:59698/143‑1 (MQ=255)                                       cTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATcc                                                                                                          >  2:198666/1‑143 (MQ=255)                                             ccGGCATACGTTCCTCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCGCGACCAATCCGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGTGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttc                                                                                                    <  2:596522/143‑1 (MQ=255)                                               ggCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCCTCATCCAGcttctt                                                                                                  >  2:728683/1‑143 (MQ=255)                                               ggCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGcttctt                                                                                                  <  2:332748/143‑1 (MQ=255)                                                gCAGACGTTCATCGATGTAATCCTGATCCAGCTAGATTTTGGGTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaacgaaaaccc                                                                                                 <  1:559586‑M2/143‑48 (MQ=255)                                                  aGACTTTCATCGATGTACTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaacgaaaaccaaa                                                                                               >  2:559586‑M2/1‑94 (MQ=255)                                                   gACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTGTTCGTTGCCCGACCAATCCGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATgg                                                                                              <  2:129633/143‑1 (MQ=255)                                                    aCGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACCTTCAGATTCTGCGCGCGCAGTGCATCGCGCAAGGCCTCCGCATCATGACGACACTCATCATCCAGCTTCTTATgag                                                                                             >  1:627086/1‑141 (MQ=255)                                                      ggtCATCGATGTAATCCTGATCCGGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGtat                                                                                           <  2:6090/141‑1 (MQ=255)                                                            cGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAgg                                                                                     >  2:363740/1‑143 (MQ=255)                                                                   aTCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCATGACGACACTGAACATCCAGCTTCTTATGGTATTGCAGGGAAacca                                                                              >  1:770350/1‑143 (MQ=255)                                                                    tCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAaccac                                                                             <  1:604761/143‑1 (MQ=255)                                                                      cTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCg                                                                           >  2:782005/1‑143 (MQ=255)                                                                      cTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACGCATCATCCAGCTGCTTATGGTATAGCAGGGCAacacccg                                                                           >  1:541693/1‑138 (MQ=255)                                                                        gATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGcc                                                                         >  2:553986/1‑143 (MQ=255)                                                                          tCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCCTATGGTATAGCAGGGAACCCACCCCCTg                                                                       >  2:115230/1‑143 (MQ=255)                                                                           ccAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGa                                                                      >  2:541802/1‑143 (MQ=255)                                                                            cAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGCAAACCACCGCCTgct                                                                     >  1:739468/1‑141 (MQ=255)                                                                                  gcttttGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGCTTCTGCGCGCGCAGTCCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCa                                                               <  2:231403/141‑1 (MQ=255)                                                                                      ttGGTTTTCGTTGCCCGACCAATCAGATGCAAATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTGATCATCCAGCTTCTTATGGTATAGCAGGGGAACCACCGCCTGATTCCTCaacgt                                                           >  2:172618/1‑139 (MQ=255)                                                                                          ttttCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCCGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTg                                                       >  1:611437/1‑143 (MQ=255)                                                                                           tttCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGa                                                      <  1:439603/143‑1 (MQ=255)                                                                                             tCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAgg                                                    <  1:373168/143‑1 (MQ=255)                                                                                               gTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTa                                                  >  1:554604/1‑143 (MQ=255)                                                                                                    ccGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCCGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGGaatcga                                             >  1:567489/1‑141 (MQ=255)                                                                                                    ccGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaa                                             >  1:341447/1‑143 (MQ=255)                                                                                                    ccGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaa                                             >  2:613864/1‑143 (MQ=255)                                                                                                     cGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGACACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTaatcaat                                            <  1:294463/143‑1 (MQ=255)                                                                                                         cAATCGGATGCACATTCAGATTCTGCGCGCGCCGTGCATCGCGCAGGGCCTACGCTTCCGGGCGCCACTCAGCGTCCAGCCTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGGGTAGTGAGGTAATCAATCTgg                                        <  2:612524/143‑1 (MQ=255)                                                                                                             cAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGaaca                                    >  2:740329/1‑143 (MQ=255)                                                                                                                aTGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTACTCAATCTGGAACAACt                                 >  1:162936/1‑143 (MQ=255)                                                                                                                aTGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACt                                 <  1:728683/143‑1 (MQ=255)                                                                                                                    acaTTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTtgtg                             <  1:198666/143‑1 (MQ=255)                                                                                                                     cagtccgATTCCGCGCGCGCAGTGCATCGCGCCGGCCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTgg                            <  1:27218/137‑1 (MQ=255)                                                                                                                       ttCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGgcg                          <  1:793618/143‑1 (MQ=255)                                                                                                                        tCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTCATCACTCTGGAACAACTTGTGgcgc                         >  2:679889/1‑143 (MQ=255)                                                                                                                        tCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTAATTACTCAGTGTAGTGAGGTAATCAACCTGGAACCACTTGTGacgc                         >  2:481353/1‑143 (MQ=255)                                                                                                                          aGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACCCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTCCTCAGCGAAGGGAGGTAATCAAGCTGGAACAACTAGTGGCCCAg                       >  2:469616/1‑143 (MQ=255)                                                                                                                              tCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACg                   >  1:698430/1‑143 (MQ=255)                                                                                                                                tGCGCGCGCAGTGCATcgcgcagaatctgaatgtgcatctgattggtcgggcaaagaattccaaaatcgagctggatcaggattacatcgatgaacgtctgccggtcgcagggaaagagatgatctaccgtcaggtagaaaac                 <  1:773866‑M2/143‑128 (MQ=255)                                                                                                                                         aGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACg        <  1:448684/143‑1 (MQ=255)                                                                                                                                               tCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACAcc  >  1:314893/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                               AAAGCTGTTTTCTACCTGACGGTAGATCATCTCTTTCCCTGCGACCGGCAGACGTTCATCGATGTAATCCTGATCCAGCTCGATTTTGGTTTTCGTTGCCCGACCAATCAGATGCACATTCAGATTCTGCGCGCGCAGTGCATCGCGCAGGGCCTCCGCTTCCTGACGCCACTCATCATCCAGCTTCTTATGGTATAGCAGGGAAACCACCGCCTGATTACTCAGTGTAGTGAGGTAATCAATCTGGAACAACTTGTGGCGCAGAACGGGATTATTACGCACACC  >  NC_000913/4162707‑4162991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.