mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ROS_KHP_14-27_CLONE_S51_L007_R1_001.good.fq | 5,799,210 | 579,296,844 | 100.0% | 99.9 bases | 100 bases | 98.2% |
| errors | ROS_KHP_14-27_CLONE_S51_L007_R2_001.good.fq | 5,799,210 | 515,758,501 | 100.0% | 88.9 bases | 89 bases | 97.8% |
| total | 11,598,420 | 1,095,055,345 | 100.0% | 94.4 bases | 100 bases | 98.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 192.4 | 5.2 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100021 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1839 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.082 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.39602 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 08:14:57 13 Jun 2021 | 08:17:47 13 Jun 2021 | 2 minutes 50 seconds |
| Read alignment to reference genome | 08:17:48 13 Jun 2021 | 08:34:42 13 Jun 2021 | 16 minutes 54 seconds |
| Preprocessing alignments for candidate junction identification | 08:34:42 13 Jun 2021 | 08:37:02 13 Jun 2021 | 2 minutes 20 seconds |
| Preliminary analysis of coverage distribution | 08:37:02 13 Jun 2021 | 08:41:15 13 Jun 2021 | 4 minutes 13 seconds |
| Identifying junction candidates | 08:41:15 13 Jun 2021 | 08:50:44 13 Jun 2021 | 9 minutes 29 seconds |
| Re-alignment to junction candidates | 08:50:44 13 Jun 2021 | 08:56:40 13 Jun 2021 | 5 minutes 56 seconds |
| Resolving best read alignments | 08:56:40 13 Jun 2021 | 09:00:04 13 Jun 2021 | 3 minutes 24 seconds |
| Creating BAM files | 09:00:04 13 Jun 2021 | 09:03:53 13 Jun 2021 | 3 minutes 49 seconds |
| Tabulating error counts | 09:03:53 13 Jun 2021 | 09:05:33 13 Jun 2021 | 1 minute 40 seconds |
| Re-calibrating base error rates | 09:05:33 13 Jun 2021 | 09:05:34 13 Jun 2021 | 1 second |
| Examining read alignment evidence | 09:05:34 13 Jun 2021 | 09:24:22 13 Jun 2021 | 18 minutes 48 seconds |
| Polymorphism statistics | 09:24:22 13 Jun 2021 | 09:24:23 13 Jun 2021 | 1 second |
| Output | 09:24:23 13 Jun 2021 | 09:25:16 13 Jun 2021 | 53 seconds |
| Total | 1 hour 10 minutes 18 seconds | ||
