Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,391,775	IS1 (+) +9 bp	coding (1259‑1267/1530 nt)	nuoM ←	NADH:quinone oxidoreductase subunit M

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1979270	NA (NA)	37 (1.030)	31/282	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2391783	0 (0.000)					coding (1259/1530 nt)	nuoM	NADH:quinone oxidoreductase subunit M
*	?	NC_000913	2391775 =	0 (0.000)	29 (0.810)	25/282	0.4	100%	coding (1267/1530 nt)	nuoM	NADH:quinone oxidoreductase subunit M
	?	NC_000913	= 3584195	NA (NA)					noncoding (768/768 nt)	IS1	repeat region

GGTCTGTGGAGCAAGATGAAATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2391916‑2391775 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG  <  NC_000913/3584195‑3584064                                                                                                                                                                                                                                                                                    tGTCTGTGAAGCCCGATCACATGGCTGCCGGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGG                                                                                                                                     <  2:568622/142‑1                ATGAAATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACT                                                                                                                      >  2:221463/1‑143                    AATGGGTGCCGGTCCTGTCGCTGTTCTTTGCGGTGGGACCGCTTGGGATGCCTGGCACCGGTAACTTCTTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATT                                                                                                                  <  2:436377/143‑1                    AATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCCTGGGATGCCTGGCACCGGTAACTTCGGCGGCGAATTTATGATTCTGTTCGGCAGCCTCCAGGTTGTACCGGAGATTAGCGGGTGCTGACTGCAACTGATT                                                                                                                  >  2:226383/1‑143                     ATGGCTGCCAGGACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTG                                                                                                                 <  1:389963/143‑1                     ATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGGTGTACCGGTGATTACCGGGTAATGAATCCAACTTGTTG                                                                                                                 >  1:272932/1‑143                               GCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATAACCGGGTAATGACTCCAACTTATTGAGAGTGTTca                                                                                                       >  2:585812/1‑141                                     cCGCTGTTCTGTGCGGTGGCAACGCGTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATTATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTC                                                                                                 <  2:299100/142‑1                                     TCGCGGTGCTTTGCGGTGGCAGCGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTC                                                                                                 <  2:335740/143‑1                                       cCTGTTCTTTGCGGTGGCAGCGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGGTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAG                                                                                               <  2:171560/142‑1                                       GCTGTTATTTGTGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAG                                                                                               <  2:96831/143‑1                                       GCTGTCCTTGGCGGTGGCAACGCTTGGGATGCCTGGCACCTGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAG                                                                                               <  2:49721/143‑1                                         TGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGGTCGGCAGCGTCCAGGTTGTACCGGTGATTCCCGGGCGATGACTCCAACTTATTGCTAGTGTTTTATGTTCAGAT                                                                                             >  2:449729/1‑143                                              TTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTGCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGGTAGTGGTTTATGTTCAGATAGTGC                                                                                        >  2:517594/1‑143                                                  CGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATACTGCCCGc                                                                                    >  2:596679/1‑142                                                          ACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTCGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTG                                                                            <  1:492299/143‑1                                                            GCTGGGGATGCCTGGCTCCGGTAACTTGGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTC                                                                          <  2:523001/143‑1                                                               TGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATG                                                                       <  1:221463/143‑1                                                                GGGAGGCCTGGCCCCGGTAACTTCGTCGGCGAATTGATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTGCCGGGGGATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGC                                                                      <  2:469924/143‑1                                                                      CCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGCTAGTGTTTTATGTTCAGATAATGCCAGATGACTTTGTCGTGCACCTCC                                                                >  2:52303/1‑143                                                                      CCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACGCATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCC                                                                >  2:138126/1‑143                                                                                GTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTG                                                      <  1:413507/143‑1                                                                                   ccagcggCGGCGACTTTCTGATATTGTCCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGA                                                   <  1:300140/136‑1                                                                                                ATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAGCGACAGCGACTT                                      >  1:182029/1‑143                                                                                                            GTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTCTGTTCCGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGCTTTTGAGAACGCCAGCGACCTCCCTCCCAGCCG                          >  1:136119/1‑143                                                                                                                 GCAGCTTCCAGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCA                     >  1:348519/1‑143                                                                                                                    GCTTCCCGGTTGTACCGGTGATTACCGGGTAATGACTCCAACTTACTGATAGTGTTTTACGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGT                  <  1:43946/143‑1                                                                                                                            GTTGTACCGGTGATTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTC          <  1:433178/143‑1                                                                                                                                    GGTGATTACCGGGTAATGACTCCAACTTACTGATCGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG  <  1:120978/143‑1                                                                                                                                                                                                                                                                                    GGTCTGTGGAGCAAGATGAAATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGCACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTGATTACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2391916‑2391775 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG  <  NC_000913/3584195‑3584064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.