New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1223991	47 (1.030)	5 (0.110)	4/252	NT	9.9%	intergenic (+84/+288)	ycgI/minE	protein YcgI/Z‑ring positioning protein MinE
	?	NC_000913	= 1223997	46 (1.050)					intergenic (+90/+282)	ycgI/minE	protein YcgI/Z‑ring positioning protein MinE

CCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGC  >  NC_000913/1223855‑1224132                                                                                                                                               |                                                                                                                                        ccGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCTACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAACGTTCTGGGGCAAAAAAAGCCCGCCAGTTAc                                                                                                                                         >  2:451550/1‑143 (MQ=255)           cgcgccGGGGAGCGCGGTGGCTGGTGGGAATTGGTCGTTGTGTCCATCAACGAGTGAACTGCGCCCCGTCTCACTTTTCGTTTAGAGGCTGTTGAAGGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAc                                                                                                                               <  2:225974/139‑1 (MQ=255)           aTCCCCGTGGTACGTGCTGGTTGGTGTTAACTGGTCGTGCCGACGATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGaagc                                                                                                                               <  2:502756/143‑3 (MQ=255)             ccccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAACAGCACAGTTCTGTTTTATAGTAGGCCCGCCAGTTACGGCGGGAAACCt                                                                                                                             >  1:617442/1‑143 (MQ=25)             ccccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGGAAGTTCTGGGGCAAAAAAAGCCCGGCAGTTACGGCGGGAAACCt                                                                                                                             >  2:887349/1‑143 (MQ=255)                   ggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACAATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAGACCTCAGCCt                                                                                                                       >  1:47783/1‑143 (MQ=255)                    gAACGCGGTTGCTGGTGTGAACTTGTCGTTCTGACCATCAACGATTAAACTGCGCTGCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATCCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTa                                                                                                                      <  1:226076/143‑1 (MQ=255)                         cgGTGGCTGGTGTGATCTGGTCGCTCTGCCTCTCAGCCATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGga                                                                                                                 <  2:398791/143‑1 (MQ=255)                            gtgCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCATCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACTGCGGGAAACCTCATCCTATGGGAGaa                                                                                                              <  2:46962/141‑1 (MQ=255)                              gCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGCAAATCCTGCGGCAAAAAAAGCCCGCCAGTTACGGCGGAAAACCTCCTCCTATGGGAGGACa                                                                                                            >  2:902939/1‑143 (MQ=39)                                 ggTGTGAGCTGGTCGTTGTGACCATCAGAAATGGAACAGCGAATTGGGGGAGTGTTGGTTAACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATg                                                                                                         <  2:358483/143‑1 (MQ=255)                                  gtgtGAACTGGTCGTTCTGACCATCAACGATTTAATTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGa                                                                                                        <  2:788682/143‑1 (MQ=255)                                  gtgtGAACTGGTCGTTCTGACCATCAACGATTAAACGGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGa                                                                                                        <  2:712221/143‑1 (MQ=255)                                    gtgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGCAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATACTATAGGAGAACAATGaat                                                                                                      >  2:648678/1‑143 (MQ=255)                                    gtgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaat                                                                                                      >  2:717894/1‑143 (MQ=255)                                      gAACTGGTCGTTCTTGCCATCAACCATTAGACTGCGCTTCGGCGCAGGTTGCGTTTACAGGATGTTGAAAGTGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaataa                                                                                                    <  2:329617/143‑1 (MQ=255)                                               gTTCTGACCATCTACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGc                                                                                           <  1:603470/143‑1 (MQ=255)                                                ttCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATCATGAAATTGCg                                                                                          >  1:810582/1‑143 (MQ=255)                                                 gcTGAGCATCAACGATTAATCTGCGCTTTGGCGCAGTTTTCGTTTACAGTATGTTGAAAGGGAAAGTTCTGTGGCATAAATATACTTCTAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCgg                                                                                         <  2:336065/142‑1 (MQ=255)                                                      ccATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGa                                                                                                 >  1:446575/1‑130 (MQ=255)                                                      ccATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGa                                                                                                 <  2:446575/130‑1 (MQ=255)                                                       cATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCCAAGGGAGAACAATGAATTATGAAATTGCGGGGTTat                                                                                   >  2:74671/1‑143 (MQ=255)                                                              gATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTcc                                                                            <  2:382932/143‑1 (MQ=255)                                                                 tgagcggcgcGTCGGCGCAGTGGTCGTTTACAGGAGGATGAAAGGGAAAATTCGGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                                                                         <  2:258708/137‑1 (MQ=255)                                                                   aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGta                                                                       >  1:842661/1‑143 (MQ=255)                                                                   aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAGTGCGGGGGTATCATCTGCGAGta                                                                       >  2:421365/1‑143 (MQ=255)                                                                        cgcTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCCCCCAGTATATcc                                                                  <  1:603650/143‑1 (MQ=255)                                                                          cTTCGGCGCAGTGTACGTGTACAGGATGTTGAAAGGGACAGCCGCCGCGCGCTTGGACCCCGTCAGTTACTGCAGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCAt                                                                <  1:865862/143‑1 (MQ=255)                                                                             cGGCGCAGTTTTCGTTGACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACt                                                             <  1:10724/143‑1 (MQ=255)                                                                               gcgtAGTGTTCGTGTGTAGCAGGTGGACAGGGAAAATTCTGGGGCGAAAGAAGCCCGCAAGTTACGGCGGGAAACGTCATCCTATGGGAGAACAATGAATAATGAAATGGCGGGGTTATCATCTCCCAGTATATCCATACTaa                                                           <  2:32662/143‑1 (MQ=255)                                                                               gcgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTaa                                                           >  1:602361/1‑143 (MQ=255)                                                                               gcgcAGTTGTCGTGTAGAGGATGTTGAAGGTGAATATTCTGGGGCAAAGAAAGCCCGCCAGTTACGGCGTGAAACCTCATCCTATGGGAGAACAATGAATAATGAAACTGCGGGGTTATCATCTCCCAGTATATCCATACTaa                                                           <  2:266442/143‑1 (MQ=255)                                                                                cgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAAc                                                          <  1:619739/143‑1 (MQ=255)                                                                                    gatttCGTTTACAGGATTGTGAAAGGGAAAAGTCTGTGTCAAAAAAGGCCCGCCAGGTACGGTGGGAAAGTTCTTCCTATGGGTGAACAATGAATAATGAAATTTCGGCGTTATCATCTTCCAGTATATCCATACTAACAATa                                                      <  2:166156/141‑1 (MQ=255)                                                                                     ttttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAAcaacaa                                                     >  1:430461/1‑143 (MQ=255)                                                                                      tttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACggcgggctttttttgccccagaattttccctttcaacatcctgtcaccgaaaactgcgccgaagcgcagtgtaatcgctgacggt                                                    <  1:44871‑M2/143‑86 (MQ=255)                                                                                      tttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACggcgggcttttttggccccagaattttccctttcaacaacctgtaaacgaaaacatcgccgaagcgcagtttaatcgtggattgt                                                    <  1:229633‑M2/143‑86 (MQ=255)                                                                                       ttCGTTGACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAgg                                                   <  1:432543/143‑1 (MQ=255)                                                                                         cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCATTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGtt                                                 >  1:491990/1‑143 (MQ=255)                                                                                         cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGtt                                                 <  1:609974/143‑1 (MQ=255)                                                                                           tttACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTAt                                               >  1:544130/1‑143 (MQ=255)                                                                                            ctACAGGATGTTGAAAGGGAATATTCTGTGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAAGGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTAtt                                              <  1:887349/142‑1 (MQ=255)                                                                                                        gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAgg                                  >  1:638792/1‑143 (MQ=255)                                                                                                        gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAgg                                  >  1:181800/1‑143 (MQ=255)                                                                                                              gAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACggcgggctttttttgccccagaattttccctttcaacatcctgtaaacgaaaactgcgccgaagcgcagtttaatcgttgatggtcagaccgaccagttcccaccagcc                            <  1:160360‑M2/143‑110 (MQ=255)                                                                                                              gAAAATGCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATaaa                            >  2:370541/1‑143 (MQ=255)                                                                                                               aaaaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTTAACCAGGCATAAAc                           >  1:563109/1‑143 (MQ=255)                                                                                                                   ttCTGTGGCAAATAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGTGGTTATCATCTCCCAGTATAGCCTTACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAttt                       <  2:745334/143‑1 (MQ=255)                                                                                                                      tGGGGCAAAGAAAGCCCGCCAGTTACGGCGAGAAACCTCAGCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAttttgt                    <  1:584343/143‑1 (MQ=255)                                                                                                                               aaaaGCCCGCCAGTTACggcgggctttttttgccccagaattttccctttcaacatcctgtaaacgaaaactgcgccgaagcgcagtttaatcgttgatggtcagaacgagcagttcacaccagccaccgcgtgccacggggg           <  1:539574‑M2/143‑127 (MQ=255)                                                                                                                                  aGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCAAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTggg        <  2:689167/143‑1 (MQ=255)                                                                                                                                   gCCCGCCAGTTACggcgggctttttttgccccagaattttccctttcaacatcctgtaaacgaaaactgcgccgaagcgcagtttaatcgttgatggtcagaacgaccagttcacaccagccaccgcgttccacggggcttcc       <  1:178005‑M2/143‑131 (MQ=255)                                                                                                                                     ccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGCTCAGGTTATTTGCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAc     <  1:384968/143‑1 (MQ=255)                                                                                                                                        ccAGTTACGGCGGGAAACCCCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACGCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGc  <  1:572236/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                        CCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGC  >  NC_000913/1223855‑1224132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.