Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	30 (0.920)	23/254	0.4	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	35 (1.080)	24/254	0.3	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979588‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA  >  NC_000913/2101749‑2101876                                                                                                                                                                                                                                         AAGAAAATTGTTTACTGATTCTCCGCTTTAAACTAGTGGTTAATATTTGTAAATCATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATT                                                                                                        <  1:652189/129‑1    AAAATATTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAATACATTTCATTATTATTTAGTTTTTTGTTGCTTTTTTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTT                                                                                                     <  1:591196/129‑1        TAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTTTTTTTTATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTCCTCG                                                                                                 <  1:204417/129‑1           TGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT                                                                                              >  1:120677/1‑129                   TTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGCTCCGCATACGCGCGGAAACTCTTCGCGCCTGACGCAGa                                                                                      >  2:633921/1‑128                      TCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCTTTTCTTTGTATTTTATTAGTTTTGTTGTGTATAAGCGAGGAAATTCTTCTCGGCTGACTCAGTCAT                                                                                   <  1:341104/129‑1                       CCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTGTCGGCTGACTCAGTCATT                                                                                  >  2:301524/1‑129                             TAAACAAGTGGTTAATATATGTACAACATTTCATTAACATTAAATTAATTATTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTT                                                                            >  1:397672/1‑129                                     TGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGT                                                                    <  2:120677/129‑1                                      GGTTAATATATGTAAAACCTTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCAGGGACATTCTTCTCGGCTCAATCAGTCATTACATTCCTTCATGTT                                                                   >  2:59607/1‑129                                         TAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTTCGTATTTTCGTTGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                <  1:57255/129‑1                                                TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                         >  2:269453/1‑129                                                TGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTATTTGTATTTAATTAGGGAGGGTGAGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                         >  2:181404/1‑129                                                      ACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGATTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCT                                                   <  1:94356/129‑1                                                       CATTTCATTAACATTAATTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGTGTAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTC                                                  <  2:653754/129‑1                                                                     TAAGTTGTTTGTTGCCTTTCTTTGTATTTAATTAAAAAAAAAAAAAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTG                                    >  1:3525/1‑129                                                                      AAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACACGCCTTca                                   >  2:590808/1‑127                                                                             TTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCAGGAAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCC                            >  1:25114/1‑129                                                                                TTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAT                         >  1:558607/1‑129                                                                                 TGCCTTTCTTTGTATTTAATTAGAGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATT                        >  2:604641/1‑129                                                                                     TTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGATGGCTCAGTCATTTCATTTCTTCATGTTTGAGCGGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCGATcaaga                    >  2:6175/1‑124                                                                                       TCTTTGTCTTTAATTAGGGAAGGTGCGAATAAACAGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACC                  >  1:300095/1‑129                                                                                             TATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAATTGCCTTGAATCAGCCTATTTAGACCGTTTCT            <  1:269453/129‑1                                                                                                TTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGACATTTCATTTCTTCATGTTTGAGCCGATTTCTTCTCCCATAAATGCCTTGAATCAGCCTATTTAGACCGCATCTTac         >  2:262655/1‑127                                                                                                   ATTAGGGTAGGTGCGAATAAGGGGGGAAATTCTTCCCGTCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAATTGCCTTGAATCAGCCTATTTAGACCGGTTCTTCGCCA      <  1:490128/129‑1                                                                                                     TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCTTATTTTTTCTTTTGTAAATGACTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATT    <  1:101054/129‑1                                                                                                     TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCTCGTAAATGTCTTTTATCAGTCTATTTAGACCGTTTCTTCGCCATT    <  1:275178/129‑1                                                                                                     TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCTTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATT    <  1:465188/129‑1                                                                                                     TAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATGTTTTCTCCCGTAAATGCCTTGAAACAGCCTATTTAGACCGTTTCTTCCCCATT    >  2:94117/1‑129                                                                                                       GGGAAGGTGGGAGTAAGCGTGGAAATTCTTCTCGGATGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGGCATTTA  <  2:452150/129‑1                                                                                                                                                                                                                                         AAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979588‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA  >  NC_000913/2101749‑2101876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.