Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	30 (0.920)	23/254	0.4	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	35 (1.080)	24/254	0.3	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979368‑1979489 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGA  <  NC_000913/2290113‑2289986                                                                                                                                                                                                                                                            AAATGGGATGTTGCGCACAGTCAAAATAACTCAACGTACATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACACACACTCACCGCACAACAAACTAAGGAAGGT                                                                                                                           >  2:254744/1‑129      GGATGTTGCGCACAGTCAAAATAACTCATCGTAAATATTCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAA                                                                                                                      <  2:213468/129‑1       GATGTTGCGCCCTGTCTAAATTACTCACCGTAAATAATCAAATGATATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCTCACAACAAACTAAGGAAGGTGCGAAC                                                                                                                     >  1:332957/1‑129       GATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATTGCTTTCATTCAATACCAGATAAGCTATTTTTAAACAGTCACTTACCGCACAACAAACTAAGGAAGGTGCGAAC                                                                                                                     <  1:205618/129‑1               GCACAGTCAAAATAACTCACCGTAAATAATCATCAGCAATAAATAATCACTTTAATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCCCACAACACACTAAGGAAGGTGCGAACAAATCCCT                                                                                                             >  1:312059/1‑129                     TCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCTCACAAGAAACTAAGGAAGGTGCGAACAAGTCCCTGATATG                                                                                                       <  1:106916/129‑1                            AACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACTCTTACCGTTTATCAAATTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCAT                                                                                                <  1:644474/129‑1                            AACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCAT                                                                                                <  2:608759/129‑1                               TCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTATCGCACAACAAACTAAGGAAGGTGCGAAGAAGTCCCTGATATGAGATCATGTT                                                                                             <  1:623106/129‑1                                 ACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTATAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTG                                                                                           >  2:430249/1‑129                                         taatagTCTGCTATAAATAATCCCTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCTTGATATGAGATCATGTTTGTCATCTGG                                                                                   <  2:562198/123‑1                                          atTCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAATCAGACACTTACCGCTCTACAAATTTATGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGA                                                                                  <  2:559131/127‑1                                             CATCTGCTATAAATAATCACTTTCATGCAATACAAGATAAGAAAGAAAAAAACAAACAATTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCC                                                                               >  1:421553/1‑129                                                  GCTATAAATACTCACTTTCATGCAATACCAGATAAAATATTTTTAAACAAACACTTAACGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGA                                                                          >  1:15768/1‑129                                                            ATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATTTTTGTTTTTGTTTGTTATTTGGAGCCATAGAACAGGGTTCA                                                                <  1:467623/129‑1                                                                 TTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATG                                                           >  2:430578/1‑129                                                                        CAATACCAGTTAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATC                                                    <  2:63642/129‑1                                                                          ATACCAGATAAGCTATTTTTAAACACACTCTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAA                                                  <  2:203946/129‑1                                                                                       TATTTTTAAACAGACACTTACCTCACAACAAACTAAAAAAAATACGAACAAGTACCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCG                                     >  1:269361/1‑129                                                                                       TATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGTTATAGAACAGGGTTCATCATGAGTCATCAACTTACCGTCGCCG                                     <  1:153322/129‑1                                                                                        ATTTTTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATTTGGAGCTATAGTACAGGGTTCATCATGAGTCATCAACGTACCTTCGCCGA                                    <  1:594443/129‑1                                                                                        ATTTTTAAACAAACACTTACCGCATAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTGTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGA                                    <  2:575457/129‑1                                                                                            TTAAACAGACACTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTCCCTTCGCCGACAGT                                >  2:378002/1‑129                                                                                             TAAACAGACACTCACCGCACAACAAACTAAGAAAGGAGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGAAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCACCGACcgcg                               >  2:62640/1‑125                                                                                                       CTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTA                                              >  2:428613/1‑104                                                                                                       CTTACCGCACAACAAACTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTA                                              <  1:428613/104‑1                                                                                                                       CTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCTTGTGAAGCCAAAGAACTGGGTTCATCATTAGTCATTAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACC     <  1:237111/129‑1                                                                                                                        tcAGGAAGTTGCGAACAAGTCCCTGAGATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCTGTAAGCGCCGTCAGACCA    <  2:41533/127‑1                                                                                                                        gcAGGAAGGTGCGAACGAGTCCCTGATATGTGATGATGTTTGTCCTCTGTTGTCATAGAACAGGGTTCATCATGAGTCATCAACTTACGTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCA    <  2:184334/127‑1                                                                                                                        TAAGGTATGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCA    <  2:542166/129‑1                                                                                                                        TAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGAGATAATCATAAAACATAAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCA    >  1:656744/1‑129                                                                                                                         AAGTAAGGTTCGAACTAGTCTCTGATATGAGATCATGTTTTTCATCTGGAGCTATAGAACATGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAG   <  2:136359/129‑1                                                                                                                         AAGGAAGGTTCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAG   <  2:335676/129‑1                                                                                                                          AGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGATCAGGGTTCATTTTGAGTTATTTTCTTACTTTCGCAGACAGTGAATTGACCAGGAAGCGCCGTCAGACCAGA  <  1:393786/129‑1                                                                                                                          AGGAAGGTGCAAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCTATAGAATAGGGTTCATCATGTGTTATTAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGA  <  1:260008/129‑1                                                                                                                                                                                                                                                            AAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979368‑1979489 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGA  <  NC_000913/2290113‑2289986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.