New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2235628	35 (0.530)	4 (0.060)	3/252	NT	9.8%	coding (364/1236 nt)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
	?	NC_000913	= 2235642	40 (0.630)					coding (378/1236 nt)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

AAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCACGCT  >  NC_000913/2235487‑2235770                                                                                                                                              |                                                                                                                                               aaaaaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCt                                                                                                                                               >  1:1113958/1‑143 (MQ=255) aaaaaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCt                                                                                                                                               >  2:1225411/1‑143 (MQ=255)  aacatatCGAGCGGATGGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATGACCCGGATCAAGTAGTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTg                                                                                                                                              <  1:712171/140‑1 (MQ=255)  aaaaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAACATTACCCGGACAAAGTAGCGATCGCTTCGATCACGGGGGAAAATGAGCAGCAACGGGAGGAGCTGGCCCGCCTg                                                                                                                                              >  2:416635/1‑143 (MQ=255)   aaagagtgAGCAGATTGCTGAACATCCGTGGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTgg                                                                                                                                             <  1:1084240/136‑1 (MQ=255)   aaaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTgg                                                                                                                                             >  2:685481/1‑143 (MQ=255)    aaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGt                                                                                                                                            >  1:626486/1‑143 (MQ=255)     agatGGGGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTg                                                                                                                                           <  2:1153395/141‑1 (MQ=255)             cAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCACTGGGAGCTGCTGGCGCGCCTGGGGCACGAAGc                                                                                                                                   >  2:314002/1‑143 (MQ=255)               gATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTg                                                                                                                                 >  1:801920‑M2/1‑142 (MQ=255)                      gAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGa                                                                                                                          >  2:836999/1‑143 (MQ=255)                       aaCATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCagcttcttgcaccaggcgcgcca                                                                                                                         <  1:733288‑M1/143‑24 (MQ=255)                       aaCATCCGTGGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCagcttcttgcaccaggcgcgcca                                                                                                                         <  1:1188801‑M1/143‑24 (MQ=255)                          aTCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATg                                                                                                                      >  1:1049250/1‑143 (MQ=255)                                tGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAg                                                                                                                <  1:685481/143‑1 (MQ=255)                                      agaATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATCACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAAc                                                                                                          <  1:1137733/143‑1 (MQ=255)                                          tCTGGCCGGCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTtct                                                                                                      <  2:614290/143‑1 (MQ=255)                                                 gccCTGCGTCGGCTGAAGGAAGGTTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTcc                                                                                               <  2:798614/143‑1 (MQ=255)                                                  ccccgtgTCGGCTGAAGGTAGATTAACCTGACTAAGTATTGATTGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCg                                                                                              <  1:69443/137‑1 (MQ=255)                                                     tGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCaa                                                                                           >  1:623770/1‑143 (MQ=255)                                                            gCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACtt                                                                                    >  1:1196328/1‑143 (MQ=255)                                                            gCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACtt                                                                                    >  1:1196125/1‑143 (MQ=255)                                                              tacaggaagATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                                                  <  1:312739/140‑1 (MQ=255)                                                              tGAAGGAATATTACCCGGACAAAGTATTGATCGCGTCGATCATGGGGGAAAATGAGCAGCAATGGGAGCAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                                                  <  1:314326/143‑1 (MQ=255)                                                                  ggaagATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                                              <  1:836999/143‑1 (MQ=255)                                                                  ggaagATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                                              >  1:899257/1‑143 (MQ=255)                                                                   gaagATTACCCGGACAAAGTATTGATCGCTTCGATGATGGGGGAAAAGGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTAGCGCGGATATGATCAAGTGTCACTTCTCCTGTCCGCCACGGACTTCTCATGc                                                                             >  2:1250996/1‑143 (MQ=255)                                                                            ccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCagcttcttgcaccaggcgcgc                                                                                                                           <  1:141281‑M1/88‑22 (MQ=255)                                                                            ccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCagcttcttgcaccaggcgcgc                                                                                                                           >  2:141281‑M1/1‑67 (MQ=255)                                                                             cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTCGc                                                                   >  2:38241/1‑143 (MQ=255)                                                                              ggACAAAGTATTGATCGCTTCGAGCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                                  <  1:745705/143‑1 (MQ=255)                                                                                 cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATg                                                               >  1:435764/1‑143 (MQ=255)                                                                                 cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATg                                                               >  1:115563/1‑143 (MQ=255)                                                                                   aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACGTCTCCTGTGCGCAAATGACTTCTCAGGCGAGGGGAAGCGATggc                                                             >  1:172397/1‑141 (MQ=255)                                                                                      tATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                                          <  1:752547/143‑1 (MQ=255)                                                                                       aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTCGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTCGCGATGTCGGGc                                                         >  1:1250678/1‑143 (MQ=255)                                                                                            tCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCATGTCGGCAAATGCCTTCTCATACGATGGGTAGCGATGTCGGGCAAAGc                                                    >  2:1102847/1‑143 (MQ=255)                                                                                               cTTCGAGCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGGGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCg                                                 <  1:936929/143‑1 (MQ=255)                                                                                                    aTCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCACGAAGCTGGCGCGGATATGATCGGGTGTAACTTCTCCTGTCCGCAAAGGACTTCTCATGCGAGGGGTCGCGCTGTCGGACAAGCCCCGgcgcc                                            >  2:124981/1‑142 (MQ=255)                                                                                                       aTGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCACATGACTTCTCATGCGATGGGTAGCGATGTGGGACACAGCCCGGAGCAGGt                                         >  2:607826/1‑143 (MQ=255)                                                                                                         gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGCGCTGGTAg                                       >  2:559817/1‑143 (MQ=255)                                                                                                          ggggAAAATGAGCATCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGCGCTGGTAGa                                      >  2:277934/1‑143 (MQ=255)                                                                                                           gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCACATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGGGCTGGGAGaa                                     >  2:858860/1‑143 (MQ=255)                                                                                                           gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa                                     >  2:351632/1‑143 (MQ=255)                                                                                                           gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAAGGGTAGCGATGTCGGGCAAAGCACGGGGCTGGTAGaa                                     >  1:1099965/1‑143 (MQ=255)                                                                                                                   gAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGGAGAAAAATATTg                             >  2:543397/1‑143 (MQ=255)                                                                                                                           aTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg                     >  2:482480/1‑143 (MQ=255)                                                                                                                               gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATCGTCGGGCAGTGaa                 >  2:149079/1‑143 (MQ=255)                                                                                                                                      tGGCGCGCCTGGTGCAAGAAGCTggcgcgccagctcctcccattgctgctcattttcccccatgatcgaagcgatcaatactttgtccgggtaatcttccttcagccgacgcagggcggccagattctcttcccacggatgtt          <  1:1188801‑M2/143‑121 (MQ=255)                                                                                                                                      tGGCGCGCCTGGTGCAAGAAGCTggcgcgccagctcctcccattgctgctcattttcccccatgatcgaagcgatcaatactttgtccgggtaatcttccttcagccgacgcagggcggccagattctcttccaacggatgtt          <  1:733288‑M2/143‑121 (MQ=255)                                                                                                                                        gcgcgcCTGGTGCAAGAAGCTggcgcgccagctcctcccattgctgctcattttcccccatgatcgaagcgatcaatactttgtccgg                                                               <  1:141281‑M2/88‑68 (MQ=255)                                                                                                                                        gcgcgcCTGGTGCAAGAAGCTggcgcgccagctcctcccattgctgctcattttcccccatgatcgaagcgatcaatactttgtccgg                                                               >  2:141281‑M2/1‑21 (MQ=255)                                                                                                                                          gcgcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAGCGGGGTTCCa      >  1:82877/1‑143 (MQ=255)                                                                                                                                            gcCTGGTGCAAGCAGCTGGCGCGGATATGATCGAGCGTAACTTCTCCTGTCCGCGACTGACTGCGCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGACAAATATTGTCGGGCAGTGAAACGGGGTTCCACg    <  2:232307/143‑1 (MQ=255)                                                                                                                                              cagcttcttgcaccaggcgcgccagctcctcccattgctgctcattttcccccatgatcgaagcgatcaatactttgtccgggtaatcttccttcagccgacgcagggcggccagattctcttccaacggatgttcagcaatc  >  1:801920‑M1/1‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               AAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGAAACGGGGTTCCACGCT  >  NC_000913/2235487‑2235770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.