breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsERR1811600_R1.good.fq609,00498,491,142100.0%161.7 bases275 bases94.3%
errorsERR1811600_R2.good.fq608,99599,540,876100.0%163.5 bases275 bases82.2%
total1,217,999198,032,018100.0%162.6 bases275 bases88.2%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0092734,631,46933.75.4100.0%Escherichia coli BW25113, complete genome.
total4,631,469100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000015246
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000106
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.013

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0092730.91291

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input16:36:23 28 Mar 202116:36:47 28 Mar 202124 seconds
Read alignment to reference genome16:36:47 28 Mar 202116:41:58 28 Mar 20215 minutes 11 seconds
Preprocessing alignments for candidate junction identification16:41:58 28 Mar 202116:42:15 28 Mar 202117 seconds
Preliminary analysis of coverage distribution16:42:15 28 Mar 202116:43:03 28 Mar 202148 seconds
Identifying junction candidates16:43:03 28 Mar 202116:43:53 28 Mar 202150 seconds
Re-alignment to junction candidates16:43:53 28 Mar 202116:44:40 28 Mar 202147 seconds
Resolving best read alignments16:44:40 28 Mar 202116:45:02 28 Mar 202122 seconds
Creating BAM files16:45:02 28 Mar 202116:45:45 28 Mar 202143 seconds
Tabulating error counts16:45:45 28 Mar 202116:46:01 28 Mar 202116 seconds
Re-calibrating base error rates16:46:01 28 Mar 202116:46:02 28 Mar 20211 second
Examining read alignment evidence16:46:02 28 Mar 202116:49:27 28 Mar 20213 minutes 25 seconds
Polymorphism statistics16:49:27 28 Mar 202116:49:28 28 Mar 20211 second
Output16:49:28 28 Mar 202116:49:42 28 Mar 202114 seconds
Total 13 minutes 19 seconds