New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 1336344	31 (0.810)	4 (0.110)	4/300	NT	13.4%	coding (167/738 nt)	pyrF	orotidine‑5'‑phosphate decarboxylase
	?	CP009273	= 1336657	22 (0.600)					coding (480/738 nt)	pyrF	orotidine‑5'‑phosphate decarboxylase

GCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCT  >  CP009273/1336086‑1336618                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   gCAACTTTTAATTTTTTATTTCCACGGGGAGAATGCTCGCCGTTGACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATCATCGTGATGACGCGCTGGNCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCTACAGTTTGTGCGGGAACtt                                                                                                                                                                                                                                                                    >  1:520424/1‑275 (MQ=255)         ttaTTTTTTATTTCCATGGGTAGAATGCTCGCAGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCg                                                                                                                                                                                                                                                            <  1:249245/273‑1 (MQ=255)                                    cTCACCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTAAACTTGTATGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTCTTGATCACTTTCTTGACCTGa                                                                                                                                                                                                                                  >  2:713991/1‑274 (MQ=255)                                            ttACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACg                                                                                                                                                                                                                         <  1:687999/275‑1 (MQ=255)                                                  gTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACCatata                                                                                                                                                                                                                    >  1:138242/1‑273 (MQ=255)                                                            aCTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGNATTCTCCTGTGGTTGTTGCTCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTcc                                                                                                                                                                                                                            <  1:557502/256‑1 (MQ=255)                                                                                      tCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAAc                                                                                                                                                                                                                                                                >  1:42572/1‑194 (MQ=255)                                                                                          gTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAgtg                                                                                                                                                                                                                                                            <  2:42572/194‑3 (MQ=255)                                                                                                                         gcgcTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAtttttgcgtcagtgccgccagacgttctgcataatctgcaggtgacagtgtcatgccaagatcgaccaggtcgctggcttc                                                                                                                                                                                                   >  1:655748‑M1/1‑139 (MQ=255)                                                                                                                             tGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAtttttgcgtcagtgccgccagacgttctgcataatctgcaggtgacagtgtcatgccaagatcgaccaggtcgctggcttccatg                                                                                                                                                                                               <  2:655739‑M1/220‑86 (MQ=255)                                                                                                                                       tctcCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGGGAGTat                                                                                                                              >  1:372262/1‑275 (MQ=255)                                                                                                                                         tcCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAtttttgcgtcagtgccgccagacgttctgcataatctgcaggtgacagtgtc                                                                                                                                                                                                                                >  1:250842‑M1/1‑123 (MQ=255)                                                                                                                                             gtgGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAtttttgcgtcagtgccgccagacgttctgcataatctgcaggtgacagtgtcatgc                                                                                                                                                                                                                            <  2:250837‑M1/175‑57 (MQ=255)                                                                                                                                                   gttgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGa                                                                                                                                                                                                                        >  1:106359/1‑173 (MQ=255)                                                                                                                                                     tgCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATcc                                                                                                                                                                                                                   >  1:438144/1‑176 (MQ=255)                                                                                                                                                       cccTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATc                                                                                                                                                                                                                    <  2:106358/173‑1 (MQ=255)                                                                                                                                                         cTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCaa                                                                                                                                                                                                               <  2:438137/176‑1 (MQ=255)                                                                                                                                                                            atgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtg                                                                                                                                                                                                                                                                               >  1:346103/1‑93 (MQ=255)                                                                                                                                                                            atgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGaa                                                                                                                                                                                                                                 >  2:382117/1‑139 (MQ=255)                                                                                                                                                                             tgaCGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtgtg                                                                                                                                                                                                                                                                             >  2:715109/1‑94 (MQ=255)                                                                                                                                                                                cgcgCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTgcg                                                                                                                                                                                                                                                                           <  2:346097/93‑1 (MQ=255)                                                                                                                                                                                cgcgCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTc                                                                                                                                                                                                                             <  1:382123/139‑1 (MQ=255)                                                                                                                                                                                 gcgcTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGcgcg                                                                                                                                                                                                                                                                         <  1:715119/94‑1 (MQ=255)                                                                                                                                                                                                    aGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTtg                                                                                                                                                                                                                                                                               >  2:499973/1‑69 (MQ=255)                                                                                                                                                                                                    aGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGTGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTAGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATTACCGCAGCGCGTGGGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGc                                                                  >  1:362019/1‑274 (MQ=255)                                                                                                                                                                                                        cGACCCGCGCGGTTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGa                                                             <  1:211827/275‑1 (MQ=255)                                                                                                                                                                                                        cGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATAATTACCTTGTTTGTGCCACAGTTTGTgcg                                                                                                                                                                                                                                                                           <  1:499980/69‑1 (MQ=255)                                                                                                                                                                                                          aCCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCtggt                                                                                                                                         >  1:616102/1‑197 (MQ=255)                                                                                                                                                                                                          aCCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCtggt                                                                                                                                         <  2:616093/197‑1 (MQ=255)                                                                                                                                                                                                                                   cGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGt                                                                                                                                                      >  2:713023/1‑159 (MQ=255)                                                                                                                                                                                                                                       aaaGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCAt                                                                                                                                                  <  1:713033/159‑1 (MQ=255)                                                                                                                                                                                                                                                tttACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGANTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCt                     <  1:595732/275‑1 (MQ=255)                                                                                                                                                                                                                                                 ttACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCNGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCt                     <  1:650117/274‑1 (MQ=255)                                                                                                                                                                                                                                                         tttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATAtctttctt                                                                                                                                                                                                                                         >  2:571503/1‑54 (MQ=255)                                                                                                                                                                                                                                                          ttGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGctgctg                                                                                                                                                                                     >  1:542428/1‑105 (MQ=255)                                                                                                                                                                                                                                                             ggCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGAcc                                                                                                                                                                                                                                     <  1:571512/54‑1 (MQ=255)                                                                                                                                                                                                                                                              gCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCGCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGc                                                                                                                                                                                 <  2:542419/105‑1 (MQ=255)                                                                                                                                                                                                                                                                   atttttgcgtcagtgccgccagacgttctgcataatctgcaggtgacagtgtcatgccaagatcgaccaggtcgctgacttccatgctggtcaacactgtcacagcaatcaaaagcggtgcatctttgccaaacggaaccagtgcctcacgcgctgcggtcatcatacgcgccccaccagaggcatgaacattcagcatccacacgcctaagtcatctgcagcagcgacagcgtgcgctgcagtgttggggatatcgtggaatttcaggacaaga  >  1:714001‑M1/1‑1 (MQ=255)                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   GCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACGTTAACTGCTTCATCTTCTTCCCGCGCTGTTACGAATTCTCCTGTGGTTGTTGCCCTTGATTATCATAATCGTGATGACGCGCTGGCCTTTGTCGACAAGATCGACCCACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGTTTACATTGTTTGGGCCACAGTTTGTGCGCGAACTTCAACAGCGTGGTTTTGATATCTTTCTTGACCTGAAATTCCACGATATCCCCAACACTGCAGCGCACGCTGTCGCTGCTGCAGCTGACTTAGGCGTGTGGATGGTGAATGTTCATGCCTCTGGTGGGGCGCGTATGATGACCGCAGCGCGTGAGGCACTGGTTCCGTTTGGCAAAGATGCACCGCTTTTGATTGCTGTGACAGTGTTGACCAGCATGGAAGCCAGCGACCTGGTCGATCTTGGCATGACACTGTCACCT  >  CP009273/1336086‑1336618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.