breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsERR3687421_R1.good.fq5,161,921606,618,691100.0%117.5 bases139 bases98.4%
total5,161,921606,618,691100.0%117.5 bases139 bases98.4%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0092734,631,46992.65.777.2%Escherichia coli BW25113, complete genome.
coveragedistributionKX0775368,1871678.423.04.1%Vector pCBB, complete sequence.
coveragedistributionExported3,34436530.01224.318.7%pZS2M_pseudoFDH_strepR_2genes.
total4,643,000100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100001
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000203
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.012

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0092730.75723
KX0775360.17324
Exported0.02972

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input20:52:12 20 Feb 202120:53:37 20 Feb 20211 minute 25 seconds
Read alignment to reference genome20:53:37 20 Feb 202121:04:33 20 Feb 202110 minutes 56 seconds
Preprocessing alignments for candidate junction identification21:04:33 20 Feb 202121:05:40 20 Feb 20211 minute 7 seconds
Preliminary analysis of coverage distribution21:05:40 20 Feb 202121:09:18 20 Feb 20213 minutes 38 seconds
Identifying junction candidates21:09:18 20 Feb 202121:09:26 20 Feb 20218 seconds
Re-alignment to junction candidates21:09:26 20 Feb 202121:16:28 20 Feb 20217 minutes 2 seconds
Resolving best read alignments21:16:28 20 Feb 202121:18:23 20 Feb 20211 minute 55 seconds
Creating BAM files21:18:23 20 Feb 202121:21:43 20 Feb 20213 minutes 20 seconds
Tabulating error counts21:21:43 20 Feb 202121:22:47 20 Feb 20211 minute 4 seconds
Re-calibrating base error rates21:22:47 20 Feb 202121:22:49 20 Feb 20212 seconds
Examining read alignment evidence21:22:49 20 Feb 202121:34:18 20 Feb 202111 minutes 29 seconds
Polymorphism statistics21:34:18 20 Feb 202121:34:20 20 Feb 20212 seconds
Output21:34:20 20 Feb 202121:35:21 20 Feb 20211 minute 1 second
Total 43 minutes 9 seconds