breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 55 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273349,9930TG37.0% 62.8 / 11.8 46V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
*CP009273595,0320TG32.3% 93.9 / 16.9 62V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*CP0092733,588,3810CA31.8% 108.3 / 12.3 63A154S (GCG→TCG) livMleucine/isoleucine/valine transporter subunit
*CP0092733,199,7940CT30.7% 160.2 / 42.9 101intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*CP009273324,5140TG29.7% 59.9 / 10.0 38T178P (ACC→CCC) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*CP0092731,010,9720GT28.6% 156.9 / 18.3 77intergenic (+57/‑199)pqiC/rmfDUF330 family putative lipoprotein/ribosome modulation factor
*CP0092732,977,8000AC28.6% 154.4 / 12.0 84G384G (GGT→GGGyqeFshort chain acyltransferase
*CP0092733,659,0620CA28.5% 261.5 / 38.5 130A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*CP009273324,5200CT28.2% 68.5 / 10.5 39D176N (GAT→AAT) ‡betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*CP009273324,5180AT27.5% 78.9 / 10.9 40D176E (GAT→GAA) ‡betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*CP0092731,009,4750AG27.4% 142.4 / 12.2 73E254G (GAA→GGA) pqiBparaquat‑inducible, SoxRS‑regulated MCE domain protein
*CP0092734,594,1610CT27.2% 213.0 / 49.2 126S62F (TCT→TTT) bglJbgl operon transcriptional activator
*CP0092732,380,3410GA26.6% 205.9 / 45.5 111intergenic (+31/‑72)yfbL/yfbMputative peptidase/DUF1877 family protein
*CP0092731,155,2930AG26.4% 143.2 / 13.3 72I572T (ATC→ACC) fhuEferric‑rhodotorulic acid outer membrane transporter
*CP0092732,372,4470CT26.3% 74.8 / 16.7 58E98K (GAA→AAA) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
*CP0092733,659,0580TC25.8% 232.3 / 33.4 130D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
*CP0092732,819,8210GA25.6% 149.6 / 23.2 78G24E (GGA→GAA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
*CP009273630,5480AT25.6% 175.2 / 21.5 82D95E (GAT→GAAybdMSpo0J family protein, ParB‑like nuclease domain
*CP0092731,483,4310GT25.5% 253.6 / 19.5 106G315C (GGT→TGT) aldAaldehyde dehydrogenase A, NAD‑linked
*CP0092733,611,7230GA25.4% 110.5 / 12.9 57K196K (AAG→AAAnikEnickel transporter subunit

Marginal new junction evidence (lowest skew 10 of 56 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 416174516 (0.170)29 (0.020)
+G
17/232 3.4 29.0% intergenic (+61/‑240) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?KX077536 8 = 127 (0.040)intergenic (–/‑1091) –/unknown –/BLA
* ? CP009273 360104 =53 (0.550)15 (0.170)
+AATTCC
12/222 4.3 25.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 4033989 = 41 (0.430)noncoding (113/120 nt) rrfA 5S ribosomal RNA of rrnA operon
* ? CP009273 4532499 =57 (0.600)14 (0.160) 11/216 4.5 14.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 107 (1.210)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 3416618 =43 (0.450)12 (0.140)
+GAATTAATTCC
9/212 5.0 24.0% intergenic (+65/+164) yhdZ/rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
?CP009273 4033989 = 41 (0.430)noncoding (113/120 nt) rrfA 5S ribosomal RNA of rrnA operon
* ? CP009273 = 403405728 (0.290)17 (0.010)
+G
10/232 5.1 18.1% intergenic (+61/+209) rrfA/mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
?KX077536 8 = 127 (0.040)intergenic (–/‑1091) –/unknown –/BLA
* ? CP009273 3416721 =36 (0.380)12 (0.010)
+G
8/232 5.7 12.9% intergenic (+168/+61) yhdZ/rrfF putative amino‑acid transporter subunit/5S ribosomal RNA of rrnD operon
?KX077536 8 = 127 (0.040)intergenic (–/‑1091) –/unknown –/BLA
* ? CP009273 = 1203261110 (1.150)6 (0.070) 6/202 5.9 6.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 90 (1.090)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 416176115 (0.160)8 (0.000)
+G
7/232 6.1 3.0% intergenic (+77/‑224) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?KX077536 24 = 501 (0.160)intergenic (–/‑1075) –/unknown –/BLA
* ? CP009273 360196 =36 (0.380)8 (0.000)
+G
7/232 6.1 2.9% intergenic (‑130/+465) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?KX077536 24 = 501 (0.160)intergenic (–/‑1075) –/unknown –/BLA
* ? CP009273 360104 =53 (0.550)6 (0.000)
+AATTCCCC
6/218 6.2 10.8% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?KX077536 8127 = NA (NA)intergenic (+52/–) unknown/– cat/–