breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 35 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091381,8410TG53.8% ‑4.2 / 13.8 13T40P (ACC→CCC) leuB3‑isopropylmalate dehydrogenase
*NC_0009132,087,4060AC50.0% 0.7 / 17.8 16W285G (TGG→GGG) yeeYputative DNA‑binding transcriptional regulator YeeY
*NC_0009131,927,8540AC46.2% 24.1 / 10.5 26V329G (GTG→GGG) ptrBoligopeptidase B
*NC_0009134,296,0600CT44.3% 30.1 / 103.8 79intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,856,6450TG43.8% 38.1 / 11.0 32K54N (AAA→AACygbAprotein YgbA
*NC_000913310,9340AC43.5% 23.6 / 11.4 23F135V (TTC→GTC) ecpRDNA‑binding transcriptional dual regulator MatA
*NC_0009134,414,9060TG43.2% 18.9 / 47.4 37V211G (GTG→GGG) aidBputative acyl‑CoA dehydrogenase AidB
*NC_0009133,233,9220GT40.7% 49.8 / 41.9 54Q75K (CAA→AAA) higAantitoxin/DNA‑binding transcriptional repressor HigA
*NC_0009131,232,4680AG40.0% 20.0 / 10.8 25intergenic (+14/+32)umuC/dsbBDNA polymerase V catalytic protein/protein thiol:quinone oxidoreductase DsbB
*NC_0009132,357,8940CA40.0% 20.8 / 16.7 25pseudogene (79/186 nt)ypaAprotein YpaA
*NC_0009131,632,5230AC37.5% 58.9 / 14.2 40intergenic (‑238/‑367)ydfJ/ynfTputative transporter YdfJ/Qin prophage; protein YnfT
*NC_0009132,280,7040AC37.5% 28.5 / 16.1 24T25P (ACC→CCC) radDputative DNA repair helicase RadD
*NC_000913424,5180AC36.0% 35.1 / 10.6 25V134G (GTG→GGG) acpHacyl carrier protein phosphodiesterase
*NC_000913821,4400CT33.3% 33.5 / 15.6 30F216F (TTC→TTTybhMBax1‑I family protein YbhM
*NC_000913859,0540GA33.3% 30.2 / 15.1 27S3F (TCT→TTT) ybiUDUF1479 domain‑containing protein YbiU
*NC_0009133,302,9280TG29.5% 87.5 / 20.5 44V147G (GTG→GGG) yhbVputative peptidase YhbV
*NC_0009133,794,1750CG28.6% 37.5 / 11.5 22G63G (GGC→GGGrfaDADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
*NC_0009132,481,9510AC27.1% 156.8 / 20.4 70L76V (TTG→GTG) emrYtripartite efflux pump membrane subunit EmrY
*NC_0009132,349,6210AC26.7% 82.9 / 32.0 45intergenic (‑149/‑66)inaA/yfaHputative lipopolysaccharide kinase InaA/putative uncharacterized protein YfaH
*NC_0009133,306,2050GT25.6% 70.4 / 22.3 43N552K (AAC→AAAdeaDATP‑dependent RNA helicase DeaD

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =48 (0.810)12 (0.230) 10/110 1.3 17.7% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 70 (1.370)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit