breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 35 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 81,841 | 0 | T | G | 53.8% | ‑4.2 / 13.8 | 13 | T40P (ACC→CCC) | leuB | 3‑isopropylmalate dehydrogenase |
* | NC_000913 | 2,087,406 | 0 | A | C | 50.0% | 0.7 / 17.8 | 16 | W285G (TGG→GGG) | yeeY | putative DNA‑binding transcriptional regulator YeeY |
* | NC_000913 | 1,927,854 | 0 | A | C | 46.2% | 24.1 / 10.5 | 26 | V329G (GTG→GGG) | ptrB | oligopeptidase B |
* | NC_000913 | 4,296,060 | 0 | C | T | 44.3% | 30.1 / 103.8 | 79 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 2,856,645 | 0 | T | G | 43.8% | 38.1 / 11.0 | 32 | K54N (AAA→AAC) | ygbA | protein YgbA |
* | NC_000913 | 310,934 | 0 | A | C | 43.5% | 23.6 / 11.4 | 23 | F135V (TTC→GTC) | ecpR | DNA‑binding transcriptional dual regulator MatA |
* | NC_000913 | 4,414,906 | 0 | T | G | 43.2% | 18.9 / 47.4 | 37 | V211G (GTG→GGG) | aidB | putative acyl‑CoA dehydrogenase AidB |
* | NC_000913 | 3,233,922 | 0 | G | T | 40.7% | 49.8 / 41.9 | 54 | Q75K (CAA→AAA) | higA | antitoxin/DNA‑binding transcriptional repressor HigA |
* | NC_000913 | 1,232,468 | 0 | A | G | 40.0% | 20.0 / 10.8 | 25 | intergenic (+14/+32) | umuC/dsbB | DNA polymerase V catalytic protein/protein thiol:quinone oxidoreductase DsbB |
* | NC_000913 | 2,357,894 | 0 | C | A | 40.0% | 20.8 / 16.7 | 25 | pseudogene (79/186 nt) | ypaA | protein YpaA |
* | NC_000913 | 1,632,523 | 0 | A | C | 37.5% | 58.9 / 14.2 | 40 | intergenic (‑238/‑367) | ydfJ/ynfT | putative transporter YdfJ/Qin prophage; protein YnfT |
* | NC_000913 | 2,280,704 | 0 | A | C | 37.5% | 28.5 / 16.1 | 24 | T25P (ACC→CCC) | radD | putative DNA repair helicase RadD |
* | NC_000913 | 424,518 | 0 | A | C | 36.0% | 35.1 / 10.6 | 25 | V134G (GTG→GGG) | acpH | acyl carrier protein phosphodiesterase |
* | NC_000913 | 821,440 | 0 | C | T | 33.3% | 33.5 / 15.6 | 30 | F216F (TTC→TTT) | ybhM | Bax1‑I family protein YbhM |
* | NC_000913 | 859,054 | 0 | G | A | 33.3% | 30.2 / 15.1 | 27 | S3F (TCT→TTT) | ybiU | DUF1479 domain‑containing protein YbiU |
* | NC_000913 | 3,302,928 | 0 | T | G | 29.5% | 87.5 / 20.5 | 44 | V147G (GTG→GGG) | yhbV | putative peptidase YhbV |
* | NC_000913 | 3,794,175 | 0 | C | G | 28.6% | 37.5 / 11.5 | 22 | G63G (GGC→GGG) | rfaD | ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase |
* | NC_000913 | 2,481,951 | 0 | A | C | 27.1% | 156.8 / 20.4 | 70 | L76V (TTG→GTG) | emrY | tripartite efflux pump membrane subunit EmrY |
* | NC_000913 | 2,349,621 | 0 | A | C | 26.7% | 82.9 / 32.0 | 45 | intergenic (‑149/‑66) | inaA/yfaH | putative lipopolysaccharide kinase InaA/putative uncharacterized protein YfaH |
* | NC_000913 | 3,306,205 | 0 | G | T | 25.6% | 70.4 / 22.3 | 43 | N552K (AAC→AAA) | deaD | ATP‑dependent RNA helicase DeaD |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4542682 = | 48 (0.810) | 12 (0.230) | 10/110 | 1.3 | 17.7% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 70 (1.370) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |