breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR396534_R1.good.fq3,892,645253,021,925100.0%65.0 bases65 bases91.9%
total3,892,645253,021,925100.0%65.0 bases65 bases91.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65246.06.5100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000001661
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500013
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.000

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.81606

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input00:51:07 24 Nov 202000:52:05 24 Nov 202058 seconds
Read alignment to reference genome00:52:06 24 Nov 202000:58:36 24 Nov 20206 minutes 30 seconds
Preprocessing alignments for candidate junction identification00:58:36 24 Nov 202000:59:16 24 Nov 202040 seconds
Preliminary analysis of coverage distribution00:59:16 24 Nov 202001:00:39 24 Nov 20201 minute 23 seconds
Identifying junction candidates01:00:39 24 Nov 202001:00:39 24 Nov 20200 seconds
Re-alignment to junction candidates01:00:39 24 Nov 202001:01:33 24 Nov 202054 seconds
Resolving best read alignments01:01:33 24 Nov 202001:02:26 24 Nov 202053 seconds
Creating BAM files01:02:26 24 Nov 202001:03:55 24 Nov 20201 minute 29 seconds
Tabulating error counts01:03:55 24 Nov 202001:04:16 24 Nov 202021 seconds
Re-calibrating base error rates01:04:16 24 Nov 202001:04:17 24 Nov 20201 second
Examining read alignment evidence01:04:17 24 Nov 202001:08:04 24 Nov 20203 minutes 47 seconds
Polymorphism statistics01:08:04 24 Nov 202001:08:05 24 Nov 20201 second
Output01:08:05 24 Nov 202001:08:14 24 Nov 20209 seconds
Total 17 minutes 6 seconds