BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	3,535,792	Δ9 bp	100%	coding (69‑77/1353 nt)	envZ ←	sensory histidine kinase EnvZ

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3535792	3535800	9	113 [0]	[0] 112	envZ	sensory histidine kinase EnvZ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3535791	0 (0.000)	107 (1.160)	82/176	NT	100%	coding (78/1353 nt)	envZ	sensory histidine kinase EnvZ
*	?	NC_000913	3535801 =	0 (0.000)	107 (1.160)	82/176	NT	100%	coding (68/1353 nt)	envZ	sensory histidine kinase EnvZ

Only 100 of 112 total aligned reads displayed.

TACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTA  >  NC_000913/3535703‑3535897

tcatcaacatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccagga                                                                                                  >  1:159346‑M2/99‑99 (MQ=255)

 catcaacatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAg                                                                                                 >  1:543901‑M2/98‑99 (MQ=255)

     aacatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAg                                                                                             >  1:1797419‑M2/94‑99 (MQ=255)

     aacatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAg                                                                                             >  2:64489‑M2/94‑99 (MQ=255)

      acatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAgg                                                                                            >  2:1151519‑M2/93‑99 (MQ=255)

      acatacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAgg                                                                                            <  1:2114933‑M2/7‑1 (MQ=255)

       catacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGt                                                                                           <  1:602935‑M2/8‑1 (MQ=255)

       catacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGt                                                                                           >  1:22215‑M2/92‑99 (MQ=255)

         tacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGa                                                                                         <  1:1025748‑M2/10‑1 (MQ=255)

         tacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGa                                                                                         <  1:1771959‑M2/10‑1 (MQ=255)

         tacgcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGa                                                                                         >  2:831214‑M2/90‑99 (MQ=255)

            gcacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGa                                                                                      >  1:2150483‑M2/87‑99 (MQ=255)

             cacttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcacgaggAGCAAGGTGACGAt                                                                                     >  2:835256‑M2/86‑99 (MQ=255)

              acttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATg                                                                                    <  2:663434‑M2/15‑1 (MQ=255)

               cttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGa                                                                                   >  2:1011596‑M2/84‑99 (MQ=255)

                ttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAg                                                                                  <  1:118299‑M2/17‑1 (MQ=255)

                ttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAg                                                                                  <  2:1595937‑M2/17‑1 (MQ=255)

                ttcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAg                                                                                  <  2:205246‑M2/17‑1 (MQ=255)

                 tcgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGc                                                                                 <  2:1236697‑M2/18‑1 (MQ=255)

                  cgtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCa                                                                                <  2:70988‑M2/19‑1 (MQ=255)

                   gtacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCaa                                                                               <  1:320370‑M2/20‑1 (MQ=255)

                    tacgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAAt                                                                              >  2:941531‑M2/79‑99 (MQ=255)

                     acgcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATa                                                                             <  1:738606‑M2/22‑1 (MQ=255)

                      cgcgaggactttattaagctgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcttcaccaggAGCAAGGTGACGATGAGCAATaa                                                                            <  1:1090868‑M2/23‑1 (MQ=255)

                       gcgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAAc                                                                           <  2:306579‑M2/24‑1 (MQ=255)

                        cgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACg                                                                          <  1:1610297‑M2/25‑1 (MQ=255)

                        cgaggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACg                                                                          <  1:1128573‑M2/25‑1 (MQ=255)

                          aggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTa                                                                        >  2:1605807‑M2/73‑99 (MQ=255)

                           ggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTAc                                                                       <  2:236759‑M2/28‑1 (MQ=255)

                           ggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTAc                                                                       >  2:471651‑M2/72‑99 (MQ=255)

                           ggactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTAc                                                                       <  1:2252181‑M2/28‑1 (MQ=255)

                            gactttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACg                                                                      >  2:1997083‑M2/71‑99 (MQ=255)

                             actttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACgg                                                                     >  2:776809‑M2/70‑99 (MQ=255)

                             actttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACgg                                                                     <  2:60121‑M2/30‑1 (MQ=255)

                              ctttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACggg                                                                    <  2:2440248‑M2/31‑1 (MQ=255)

                               tttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGc                                                                   <  2:1277273‑M2/32‑1 (MQ=255)

                               tttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGc                                                                   >  1:1054950‑M2/68‑99 (MQ=255)

                               tttattaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGc                                                                   <  1:2161992‑M2/32‑1 (MQ=255)

                                  attaaactgctggcggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCaaa                                                                <  2:1458147‑M2/35‑1 (MQ=255)

                                  attaaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCaaa                                                                >  1:1338355‑M2/65‑99 (MQ=255)

                                     aaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGa                                                             >  2:1054529‑M2/62‑99 (MQ=255)

                                     aaactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGa                                                             <  1:1516733‑M2/38‑1 (MQ=255)

                                      aactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGaa                                                            <  2:1809629‑M2/39‑1 (MQ=255)

                                      aactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGaa                                                            <  2:1467616‑M2/39‑1 (MQ=255)

                                      aactgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGaa                                                            >  2:1006095‑M2/61‑99 (MQ=255)

                                       actgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAAc                                                           <  1:1938763‑M2/40‑1 (MQ=255)

                                       actgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAAc                                                           >  2:2282294‑M2/60‑99 (MQ=255)

                                        ctgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACt                                                          >  1:296699‑M2/59‑99 (MQ=255)

                                        ctgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACt                                                          <  2:2284943‑M2/41‑1 (MQ=255)

                                         tgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                         <  1:495275‑M2/42‑1 (MQ=255)

                                         tgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                         <  1:2189550‑M2/42‑1 (MQ=255)

                                         tgctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACtt                                                         <  1:1494920‑M2/42‑1 (MQ=255)

                                          gctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTc                                                        <  1:252476‑M2/43‑1 (MQ=255)

                                           ctggaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCg                                                       <  1:324050‑M2/44‑1 (MQ=255)

                                              gaggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTgg                                                    <  1:1225529‑M2/47‑1 (MQ=255)

                                                ggctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCg                                                  >  1:2325790‑M2/51‑99 (MQ=255)

                                                 gctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCga                                                 <  1:2159648‑M2/50‑1 (MQ=255)

                                                 gctcggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCga                                                 >  2:1647929‑M2/50‑99 (MQ=255)

                                                    cggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGaa                                              <  2:670467‑M2/53‑1 (MQ=255)

                                                     ggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAg                                             <  2:2069111‑M2/54‑1 (MQ=255)

                                                     ggcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAg                                             >  2:1492817‑M2/46‑99 (MQ=255)

                                                      gcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAgc                                            >  1:148902‑M2/45‑99 (MQ=255)

                                                      gcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAgc                                            >  1:1848931‑M2/45‑99 (MQ=255)

                                                      gcaaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAgc                                            >  1:1866504‑M2/45‑99 (MQ=255)

                                                       caaaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAgcg                                           <  2:773043‑M2/56‑1 (MQ=255)

                                                         aaatcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCa                                         >  1:480925‑M2/42‑99 (MQ=255)

                                                           atcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAAt                                       <  1:170610‑M2/60‑1 (MQ=255)

                                                           atcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAAt                                       >  1:1243848‑M2/40‑99 (MQ=255)

                                                           atcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAAt                                       >  1:1111917‑M2/40‑99 (MQ=255)

                                                           atcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAAt                                       <  1:2094245‑M2/60‑1 (MQ=255)

                                                           atcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAAt                                       <  1:2344490‑M2/60‑1 (MQ=255)

                                                            tcgcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATc                                      >  2:1462882‑M2/39‑99 (MQ=255)

                                                              gcgaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGc                                    >  1:754586‑M2/37‑99 (MQ=255)

                                                                gaagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCt                                  >  1:595650‑M2/35‑99 (MQ=255)

                                                                 aagttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTc                                 >  1:1866338‑M2/34‑99 (MQ=255)

                                                                  agttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCa                                >  1:647611‑M2/33‑99 (MQ=255)

                                                                  agttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCa                                <  2:2070822‑M2/67‑1 (MQ=255)

                                                                  agttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCa                                <  1:863644‑M2/67‑1 (MQ=255)

                                                                  agttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCa                                >  2:340299‑M2/33‑99 (MQ=255)

                                                                    ttcagcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATAGCCTCATg                              >  2:730374‑M2/31‑99 (MQ=255)

                                                                       agcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCtt                           >  1:441870‑M2/28‑99 (MQ=255)

                                                                        gcaccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCttt                          >  2:395330‑M2/27‑99 (MQ=255)

                                                                         caccaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTa                         <  2:1536500‑M2/74‑1 (MQ=255)

                                                                          accaccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTag                        <  2:821046‑M2/75‑1 (MQ=255)

                                                                            caccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTagag                      <  2:1018457‑M2/77‑1 (MQ=255)

                                                                            caccagataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTagag                      >  2:1031777‑M2/23‑99 (MQ=255)

                                                                                 gataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTc                 <  2:372623‑M2/82‑1 (MQ=255)

                                                                                 gataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTc                 <  1:400963‑M2/82‑1 (MQ=255)

                                                                                 gataagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTc                 <  1:2262148‑M2/82‑1 (MQ=255)

                                                                                   taagtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCg               >  2:794494‑M2/16‑99 (MQ=255)

                                                                                     agtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGt             >  2:596529‑M2/14‑99 (MQ=255)

                                                                                     agtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGt             >  1:1988690‑M2/14‑99 (MQ=255)

                                                                                      gtcgtcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAACTGAACTTCGTGGCGAGAAGCGAAATCGCCTCATGCTTTAGAGCCGTCCGGTa            <  2:629793‑M2/87‑1 (MQ=255)

                                                                                          tcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACaaa        >  1:1416248‑M2/9‑99 (MQ=255)

                                                                                          tcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACaaa        <  2:1048091‑M2/91‑1 (MQ=255)

                                                                                          tcaccaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACaaa        <  2:844098‑M2/91‑1 (MQ=255)

                                                                                            accaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGa      <  1:1429784‑M2/93‑1 (MQ=255)

                                                                                            accaggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGa      >  2:1514766‑M2/7‑99 (MQ=255)

                                                                                               aggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGt   <  1:207804‑M2/96‑1 (MQ=255)

                                                                                                ggAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTa  >  1:966231‑M2/3‑99 (MQ=255)

TACGCACTTCGTACGCGAGGACTTTATTAAACTGCTGGAGGCTCGGCAAAATCGCGAAGTTCAGCACCACCAGATAAGTCGTCACCAGGCTGGCGAACAGCAAGGTGACGATGAGCAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTA  >  NC_000913/3535703‑3535897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`