breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 70,581 C→T 5.3% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator AraC
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
MC JC 1,397,381 Δ13,756 bp 100% [ynaJ][ttcA] 16 genes
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,866,713 A→C 7.1% L280W (TTG→TGG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,867,169 A→T 16.2% I128N (ATC→AAC)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 4,296,060 C→T 28.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,542,237 IS1 (+) +9 bp 61.3% coding (201‑209/597 nt) fimE → regulator for fimA
JC JC 4,542,448 IS5 (–) +4 bp 5.5% coding (412‑415/597 nt) fimE → regulator for fimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423699–3424234 3424600–3424238 5–902 66 [65] [64] 66 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =51 (0.440)54 (0.570) 44/144 NT 59.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 33 (0.350)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780552 (0.450)58 (0.620) 46/144 NT 60.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 33 (0.350)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994981 (0.010)100 (0.910) 75/168 NT 99.5% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 4542682 =54 (0.470)83 (0.800) 58/158 NT 60.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 60 (0.580)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269054 (0.470)125 (1.210) 82/158 NT 69.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 60 (0.580)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit