Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,003,479	(G)6→9	coding (866/879 nt)	xdhB →	putative xanthine dehydrogenase FAD‑binding subunit XdhB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3003473	2 (0.050)	37 (0.860) +GGG	33/258	0.2	94.9%	coding (860/879 nt)	xdhB	putative xanthine dehydrogenase FAD‑binding subunit XdhB
	?	NC_000913	3003474 =	2 (0.050)					coding (861/879 nt)	xdhB	putative xanthine dehydrogenase FAD‑binding subunit XdhB

GCAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3003336‑3003473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTAAGTG  >  NC_000913/3003474‑3003606                                                                                                                                           |||                                                                                                                                      GCAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGG                                                                                                                                        <  1:688699/140‑1      CGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGG                                                                                                                                   <  2:26002/140‑1        CTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGG                                                                                                                                 >  2:431798/1‑140                TATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCA                                                                                                                        <  1:431798/140‑1                     GCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGATTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGAGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGGAAATTTG‑AATGAA                                                                                                                   >  1:504316/1‑140                                         GTCGCCCCGCGTTCTTCATGGCGGGTCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCA                                                                                               <  1:299347/140‑1                                                CGCGTTCTTCATGGCGGGCCAGTAAAGAGGTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGGGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGGAAAA‑TGGAAAGAAATACAGCGAAACAAATACCCTCGAAag                                                                                        >  2:401812/1‑138                                                                 GCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGG‑AAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGC                                                                       >  1:69624/1‑140                                                                           AGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCT                                                             >  2:575787/1‑140                                                                           AGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCT                                                             <  1:162095/140‑1                                                                             TTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAAGGCACCATTAACGGGATGGCTTTTCAGCTTC                                                           >  1:723501/1‑140                                                                              TTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCA                                                          >  1:539770/1‑140                                                                               TCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCACCGAATGCTCCAATAACGGGATACCTTTTCAGCTTCAC                                                         >  1:230214/1‑140                                                                                CGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACG                                                        >  1:693362/1‑140                                                                                CGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACG                                                        <  2:69624/140‑1                                                                                  TCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCC                                                      >  1:549574/1‑140                                                                                  TCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCC                                                      >  1:524631/1‑140                                                                                         CTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAG                                               >  1:650783/1‑140                                                                                                AGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCC                                        <  1:680246/140‑1                                                                                                AGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCC                                        >  2:432644/1‑140                                                                                                 GACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCG                                       <  2:403674/140‑1                                                                                                   gGATGACCAAAAAAGTGATTAGCGAACCCGTCGCCGCGG‑GGGGGGGGGAAAATTGCAATGAATCACAGCGAAAAAATTCCCATCGACTGCACCATTAACGGGATGCCCTTTCAGCTTCACGCCGCACCAGGCACGCCGCT                                     <  2:504316/139‑1                                                                                                      TGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATAGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCT                                   <  2:58340/140‑1                                                                                                      TGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATAGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCT                                   >  1:58340/1‑140                                                                                                      TGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTC                                  >  2:456588/1‑140                                                                                                       GACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATAAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCG                                 >  1:185309/1‑140                                                                                                           AAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATAAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAAT                             <  2:185309/140‑1                                                                                                            AAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATT                            >  2:518843/1‑140                                                                                                            AAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATT                            <  2:549574/140‑1                                                                                                            AAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCC                                        <  2:38811/128‑1                                                                                                            AAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCC                                        >  1:38811/1‑128                                                                                                                  TGATTAGCGAAGCCGTCCCCGCCGC‑GGGGGGGGAAGATTACAATGAATTACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCG                      <  2:230214/140‑1                                                                                                                       AGCGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAAC                 <  1:456588/140‑1                                                                                                                         CGAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAGTTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAA               <  1:563834/140‑1                                                                                                                          GAAGCCGTCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAG              <  2:693362/140‑1                                                                                                                                 TCGCCGGGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCT       <  1:616717/140‑1                                                                                                                                 TCGCCGCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCT       >  1:662134/1‑140                                                                                                                                      GCGGC‑GGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTAAGTG  <  1:575787/140‑1                                                                                                                                           |||                                                                                                                                      GCAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3003336‑3003473 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTAAGTG  >  NC_000913/3003474‑3003606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.