Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,468,599	Δ4 bp	coding (386‑389/921 nt)	yfdH →	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2468599	2468602	4	58 [0]	[0] 55	yfdH	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2468598	0 (0.000)	55 (1.120)	43/276	0.1	100%	coding (385/921 nt)	yfdH	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
	?	NC_000913	2468603 =	0 (0.000)					coding (390/921 nt)	yfdH	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

GGATGCGATAATCCCAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTC  >  NC_000913/2468468‑2468741                                                                                                                                        |                                                                                                                                           ccggggatgcgataattccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatctggttcttgctaaaagatctgaccgctcaactgatggacgccGCGaa                                                                                                                                        <  2:513777‑M2/5‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  1:831432‑M2/16‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  1:169718‑M2/16‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  2:555666‑M2/16‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  1:617307‑M2/16‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  1:49134‑M2/16‑1 (MQ=255)            ataatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAg                                                                                                                             <  2:21824‑M2/16‑1 (MQ=255)              aatcccaattgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTg                                                                                                                           <  1:501198‑M2/18‑1 (MQ=255)                      ttgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCtata                                                                                                                   <  1:679015‑M2/26‑1 (MQ=255)                      ttgatgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCtata                                                                                                                   >  2:117457‑M2/115‑140 (MQ=255)                          tgttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCt                                                                                                               <  2:675143‑M2/30‑1 (MQ=255)                           gttgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTGTAAGCTc                                                                                                              >  2:113290‑M2/110‑140 (MQ=255)                             tgacctgcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacaccGTGAAAAACGGCTGACTGGTTCTATAAGCTCca                                                                                                            >  2:332920‑M2/108‑140 (MQ=255)                                   gcaagacccgattgaggttattcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataa                                                                                                      >  2:422610‑M2/102‑140 (MQ=255)                                    caagacccgattgaggttattcctcatcttattgagaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGCGTCGTTCTAGAAGCTCCACaataaa                                                                                                     >  1:385443‑M2/101‑140 (MQ=255)                                                 aggttattcctcatcttattgacaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTa                                                                                        <  1:374708‑M2/53‑1 (MQ=255)                                                    ttattcctcatcttattgaaaactggcaagcaggtcctgctatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTaaaa                                                                                     <  2:282162‑M2/56‑1 (MQ=255)                                                    ttattcctcatcttattcaaaaatggcaagctggtcctgttatggttcctgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTaaaa                                                                                     <  2:232360‑M2/56‑1 (MQ=255)                                                      attcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAg                                                                                                >  1:32023‑M2/83‑127 (MQ=255)                                                      attcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAg                                                                                                <  2:32023‑M2/45‑1 (MQ=255)                                                        tcctcatcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGATGAGTGGTTCTAAAAGGTCCACAATTAAATAAGCAATCCTAAAATTGa                                                                                 >  2:249705‑M2/81‑140 (MQ=255)                                                         cctcatcttattgaacaatggccagcaggcgctgatacggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGaa                                                                                <  1:247282‑M2/61‑1 (MQ=255)                                                         cctcatcttattgaaaaatggcaagtaggagctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGaa                                                                                <  1:332920‑M2/61‑1 (MQ=255)                                                             atcttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAgaga                                                                            <  1:76621‑M2/65‑1 (MQ=255)                                                                ttattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGATCTATAAGCTCCGCAATAAAATAAGCAATCCTAAAATTGAAGAGAATg                                                                         >  2:261520‑M2/73‑140 (MQ=255)                                                                 tattgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGt                                                                        >  1:31820‑M2/72‑140 (MQ=255)                                                                  attgaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGtt                                                                       >  2:640057‑M2/71‑140 (MQ=255)                                                                     gaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGt                                                                    >  2:126032‑M2/68‑140 (MQ=255)                                                                     gaaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgacagctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGt                                                                    <  1:729673‑M2/73‑1 (MQ=255)                                                                      aaaaatggcaagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTg                                                                   >  1:736184‑M2/67‑140 (MQ=255)                                                                              caagcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAgg                                                           <  1:117457‑M2/82‑1 (MQ=255)                                                                                agcaggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCt                                                         >  2:139085‑M2/57‑140 (MQ=255)                                                                                   aggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCtgat                                                      <  1:657955‑M2/87‑1 (MQ=255)                                                                                    ggtgctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTGTAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCtgatg                                                     <  1:113290‑M2/88‑1 (MQ=255)                                                                                       gctgatatggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGc                                                  >  1:171320‑M2/50‑140 (MQ=255)                                                                                              tggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGAtg                                           <  1:185667‑M2/98‑1 (MQ=255)                                                                                               ggttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAAAGAATGTTGGTCATGTCAGGCTGATGAGCCGTGAtgt                                          >  1:383330‑M2/42‑140 (MQ=255)                                                                                                gttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGAtgtt                                         >  1:591430‑M2/41‑140 (MQ=255)                                                                                                gttcttgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTGGGTGATTTCAGGTGGATGAGCTGTGAtgtt                                         >  2:378773‑M2/41‑140 (MQ=255)                                                                                                     tgctaaaagatctgagcgctcacctgatgaacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                    <  1:374677‑M2/105‑1 (MQ=255)                                                                                                     tgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                    <  2:736184‑M2/105‑1 (MQ=255)                                                                                                     tgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                    <  1:181109‑M2/105‑1 (MQ=255)                                                                                                     tgctaaaagatctgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                    >  1:444637‑M2/36‑140 (MQ=255)                                                                                                       ctaaaagatctgacccctcaaccgaaggatgccGCGACAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGaaa                                  <  1:249705‑M2/107‑1 (MQ=255)                                                                                                                 tgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATAtt                             <  1:201984‑M2/112‑1 (MQ=255)                                                                                                                 tgaccgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATAtt                             >  2:201984‑M2/24‑135 (MQ=255)                                                                                                                   accgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTa                      >  2:26551‑M2/22‑140 (MQ=255)                                                                                                                   accgctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTAGTGATTTCCGGCTGATCAGCCGTGATGTTGTCGAAAATATTAAACTTa                      >  2:311996‑M2/22‑140 (MQ=255)                                                                                                                       ctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGGTGAGCCGAGATATTATCGGAAGTATTAAACTTATGcc                  >  1:360559‑M2/18‑140 (MQ=255)                                                                                                                       ctcaactgatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGcc                  <  1:139085‑M2/123‑1 (MQ=255)                                                                                                                              gatggacgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGa           <  1:26551‑M2/130‑1 (MQ=255)                                                                                                                                    cgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTGAACTTATGCCAGAACGAAACCtt     >  2:176791‑M2/5‑140 (MQ=255)                                                                                                                                    cgccGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCtt     <  2:591430‑M2/136‑1 (MQ=255)                                                                                                                                    cgccGCGAAAAACGGCACAGTGCTTCTATACGCTCGACAGTAAAATAAACAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCtt     <  2:383330‑M2/136‑1 (MQ=255)                                                                                                                                       cGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTc  >  2:134716‑M2/2‑140 (MQ=255)                                                                                                                                        |                                                                                                                                           GGATGCGATAATCCCAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTC  >  NC_000913/2468468‑2468741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑