Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	634,250	Δ13 bp	coding (665‑677/1161 nt)	ybdL →	methionine‑oxo‑acid transaminase, PLP‑dependent

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	634250	634262	13	43 [0]	[0] 44	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 634249	0 (0.000)	43 (0.730)	36/276	0.5	100%	coding (664/1161 nt)	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent
	?	NC_000913	634263 =	0 (0.000)					coding (678/1161 nt)	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent

ATTTCGCCGCTTTGTGGCAGGCGATCGCCGGGCACGAGATTTTTGTCATTAGCGATGAAGTCTACGAGCACATCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGG  >  NC_000913/634136‑634402                                                                                                                                |                                                                                                                                            tggcagcaggctgatttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCg                                                                                                                                 >  2:555498‑M2/128‑140 (MQ=255)       caggctgatttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCgggtttt                                                                                                                           >  2:34030‑M2/122‑138 (MQ=255)       caggctgatttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTttct                                                                                                                           >  2:485216‑M2/122‑140 (MQ=255)         ggctgatttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGGGGCGGTtttttc                                                                                                                         >  2:948653‑M2/120‑140 (MQ=255)          gctgatttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCa                                                                                                                        <  1:33774‑M2/22‑1 (MQ=255)                ttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATttgac                                                                                                                  <  1:688892‑M2/28‑3 (MQ=255)                ttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGc                                                                                                                  <  1:34030‑M2/28‑1 (MQ=255)                ttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGc                                                                                                                  <  2:81041‑M2/28‑1 (MQ=255)                ttcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGc                                                                                                                  <  1:555498‑M2/28‑1 (MQ=255)                 tcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCa                                                                                                                 <  1:928387‑M2/29‑1 (MQ=255)                 tcgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTATTCATTTGGCa                                                                                                                 <  2:521022‑M2/29‑1 (MQ=255)                  cgccgctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCaa                                                                                                                <  1:171576‑M2/30‑1 (MQ=255)                      gctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTTGCAAGAcc                                                                                                            >  1:783710‑M2/107‑140 (MQ=255)                      gctttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc                                                                                                            <  1:909397‑M2/34‑1 (MQ=255)                        tttgtggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTa                                                                                                          <  2:305294‑M2/36‑1 (MQ=255)                            tggcaggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTTTTTCTTTGGGAAAAACTATCat                                                                                                      >  2:975086‑M2/101‑140 (MQ=255)                                 ggcgatcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttctcaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGAc                                                                                                 <  1:220135‑M2/45‑1 (MQ=255)                                      tcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCt                                                                                            <  1:485216‑M2/50‑1 (MQ=255)                                      tcgccgggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCt                                                                                            >  2:633684‑M2/91‑140 (MQ=255)                                            ggcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAg                                                                                      >  1:868718‑M2/85‑140 (MQ=255)                                             gcacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGt                                                                                     <  2:16102‑M2/57‑1 (MQ=255)                                              cacgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTg                                                                                    >  2:245028‑M2/83‑140 (MQ=255)                                                cgagatttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTggg                                                                                  <  1:949331‑M2/60‑1 (MQ=255)                                                      ttttgtcattagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATtg                                                                            <  1:633684‑M2/66‑1 (MQ=255)                                                             attagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGGAAGGGGGGTATTTTGTTtcg                                                                     >  2:270979‑M2/68‑140 (MQ=255)                                                              ttagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGGAAGTGGGGTATTGTGTTgcgc                                                                    >  1:864832‑M2/67‑140 (MQ=255)                                                              ttagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgcgc                                                                    <  1:975086‑M2/74‑1 (MQ=255)                                                               tagcgatgaagtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTTGCAAGACCTATCATATGACCAGCTGGAAAGTGGGGTATTGTGTTGCGcc                                                                   >  1:703166‑M2/66‑140 (MQ=255)                                                                        agtctacgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCAt                                                          <  2:480504‑M2/84‑1 (MQ=255)                                                                              cgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTTATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTTTTTTGCGCCAGCGCCCCTCAgcgc                                                    >  2:189388‑M2/51‑140 (MQ=255)                                                                              cgagcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAgcgc                                                    <  1:432452‑M2/90‑1 (MQ=255)                                                                                 gcacatcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGa                                                 <  2:868718‑M2/93‑1 (MQ=255)                                                                                    catcaacttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAAt                                              >  2:195951‑M2/45‑140 (MQ=255)                                                                                         acttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCa                                         <  1:189388‑M2/101‑1 (MQ=255)                                                                                         acttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCa                                         <  2:645765‑M2/101‑1 (MQ=255)                                                                                         acttttcacaacagggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCa                                         <  1:375773‑M2/101‑1 (MQ=255)                                                                                                       ggccatgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGGAAGTGGGGTATTGTGTTTCGCCCGCGCCCATCAGCGCCGAAATTCGCAAGGGACATCAGTAt                           >  2:311853‑M2/26‑140 (MQ=255)                                                                                                            tgccagtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGAc                      >  2:801966‑M2/21‑140 (MQ=255)                                                                                                                agtgtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCttt                  <  1:420108‑M2/124‑1 (MQ=255)                                                                                                                   gtgctggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCg               <  1:801966‑M2/127‑1 (MQ=255)                                                                                                                       tggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTTGCAAGACCTATCATATGACCGGCTGGGAAGGGGGGTATTTTGTTGCGCCAGCGCCCATCAGCGCCGGAATTCGCAAGGGACATCAGTATCTTACCTTTTCGGTGa           >  2:420108‑M2/10‑140 (MQ=255)                                                                                                                        ggcgcatcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa          <  1:35249‑M2/132‑1 (MQ=255)                                                                                                                             atcGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATAccc     <  1:948653‑M2/137‑1 (MQ=255)                                                                                                                                gCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCgg  <  1:232497/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                            ATTTCGCCGCTTTGTGGCAGGCGATCGCCGGGCACGAGATTTTTGTCATTAGCGATGAAGTCTACGAGCACATCAACTTTTCACAACAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGG  >  NC_000913/634136‑634402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑