New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3588424 =	58 (1.070)	5 (0.100)	5/250	4.7	8.6%	coding (757/1071 nt)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
	?	NC_000913	3589222 =	53 (1.070)					coding (806/846 nt)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATGGTGATGAACGGCGGTGTTGCCGAACAGATTGGCACACCAGTTGAAGTCTACGAAAAGCCCGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3588565‑3588424 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgaaggcacgcTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGG  >  NC_000913/3589222‑3589360                                                                                                                                                                                                                                                                                           ATGGTGATGAACGGCGGTGTTGCCGAACAGATTGGCACACCAGTTGAAGTCTACGAAAAGCCCGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCAC                                                                                                                                               >  2:519964/1‑140                                                 GTCTACGAAAAGCCCGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACA                                                                                               >  2:54373/1‑140                                                            GCCCGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATC                                                                                    >  2:538642/1‑140                                                               CGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACT                                                                                 <  1:54373/140‑1                                                                                                                    CAGGCCGCGTGAATAACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGAT                                  <  1:747201/134‑1                                                                                                                    CAGGCCGCGTGAATAACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGAT                                  >  2:747201/1‑134                                                                                                                                      AAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATC          >  2:1244/1‑140                                                                                                                                      AAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCG               <  1:249818/135‑1                                                                                                                                      AAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCG               >  2:249818/1‑135                                                                                                                                         GCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCAC       <  2:488161/140‑1                                                                                                                                           ACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACAT     <  1:220848/140‑1                                                                                                                                              CTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGG  <  1:656304/140‑1                                                                                                                                              CTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGG  <  2:89372/140‑1                                                                                                                                                                                                                                                                                           ATGGTGATGAACGGCGGTGTTGCCGAACAGATTGGCACACCAGTTGAAGTCTACGAAAAGCCCGCCAGCCTGTTTGTAGCGAGTTTTATCGGCAGTCCGGCGATGAACCTGCTGACAGGCCGCGTGAATAACGAAGGCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3588565‑3588424 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgaaggcacgcTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGG  >  NC_000913/3589222‑3589360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.