New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3588424 =	58 (1.070)	5 (0.100)	5/250	4.7	8.6%	coding (757/1071 nt)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
	?	NC_000913	3589222 =	53 (1.070)					coding (806/846 nt)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCATCCGCCACATCAAGGGTCAGCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGT  >  NC_000913/3589092‑3589361                                                                                                                                   |                                                                                                                                            ccATCCGCCACATCAAGGGTCAGCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTa                                                                                                                                    >  2:649662/1‑140 (MQ=255)                aGGGTCAGCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCg                                                                                                                     >  2:327963/1‑140 (MQ=255)                      aGCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACATTCACCACCGGAGGGa                                                                                                               >  1:866606/1‑140 (MQ=255)                       gCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAGGGCACGCTGCATCACTAAAACAATcac                                                                                                                          <  2:903397/128‑1 (MQ=255)                       gCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAGGGCACGCTGCATCACTAAAACAATcac                                                                                                                          >  1:903397/1‑128 (MQ=255)                       gCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGAt                                                                                                              <  2:920986/140‑1 (MQ=255)                       gCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGAt                                                                                                              <  1:327963/140‑1 (MQ=255)                        cGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATa                                                                                                             >  1:165699/1‑140 (MQ=255)                           tttAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGc                                                                                                          >  2:904043/1‑140 (MQ=255)                             tAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGt                                                                                                        >  2:405960/1‑140 (MQ=255)                             tAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGt                                                                                                        >  1:508114/1‑140 (MQ=255)                                     tGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAacaaca                                                                                                >  2:801262/1‑140 (MQ=255)                                     tGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAacaaca                                                                                                <  1:858046/140‑1 (MQ=255)                                               cATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCAt                                                                                      <  1:904043/140‑1 (MQ=255)                                                 tCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCa                                                                                    >  2:656304/1‑140 (MQ=255)                                                 tCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCa                                                                                    >  1:618287/1‑140 (MQ=255)                                                   ccAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACt                                                                                  >  1:797152/1‑140 (MQ=255)                                                      gCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAg                                                                               <  1:325235/140‑1 (MQ=255)                                                      gCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAg                                                                               <  2:556311/140‑1 (MQ=255)                                                      gCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAg                                                                               >  1:901865/1‑140 (MQ=255)                                                                 cTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgt                                                                    >  2:44436/1‑140 (MQ=255)                                                                 cTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgt                                                                    >  1:424787/1‑140 (MQ=255)                                                                 cTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgt                                                                    >  1:850671/1‑140 (MQ=255)                                                                 cTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgt                                                                    >  1:433218/1‑140 (MQ=255)                                                                 cTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgt                                                                    >  1:326971/1‑140 (MQ=255)                                                                  tGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACt                                                                                  <  1:780270/125‑1 (MQ=255)                                                                  tGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACt                                                                                  >  2:780270/1‑125 (MQ=255)                                                                     cTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCgtggtg                                                                <  2:165699/140‑1 (MQ=255)                                                                        tAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCt                                                             >  2:523941/1‑140 (MQ=255)                                                                              cAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCTACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCt                                                       >  1:506447/1‑140 (MQ=255)                                                                              cAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCt                                                       >  2:747291/1‑140 (MQ=255)                                                                                   cTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGc                                                  >  1:556754/1‑140 (MQ=255)                                                                                     gCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGa                                                >  2:699257/1‑140 (MQ=255)                                                                                      ccATCTTATTTCTCACTATCGACCATGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGat                                               >  2:220848/1‑140 (MQ=255)                                                                                      ccATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGat                                               >  1:170219/1‑140 (MQ=255)                                                                                        aTCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGatca                                             <  2:850671/140‑1 (MQ=255)                                                                                         tCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGatcat                                            <  2:508114/140‑1 (MQ=255)                                                                                         tCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGatcat                                            <  1:405960/140‑1 (MQ=255)                                                                                                    aCTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATTATCCCTTTGATcc                                 >  2:731073/1‑140 (MQ=255)                                                                                                    aCTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATcc                                 >  1:162171/1‑140 (MQ=255)                                                                                                    aCTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATcc                                 >  1:601621/1‑140 (MQ=255)                                                                                                    aCTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCATCGCCTGTAGCGATCATCCCTTTGATcc                                 >  2:119491/1‑140 (MQ=255)                                                                                                     cTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATccc                                <  2:861467/140‑1 (MQ=255)                                                                                                        tCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGc                             >  1:280598/1‑140 (MQ=255)                                                                                                          gACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCa                           <  1:313492/140‑1 (MQ=255)                                                                                                           aCCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCAc                          >  1:141009/1‑140 (MQ=255)                                                                                                           aCCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCAc                          >  1:99961/1‑140 (MQ=255)                                                                                                           aCCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCAc                          >  1:488161/1‑140 (MQ=255)                                                                                                               ggCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACggtg                      <  1:649662/140‑1 (MQ=255)                                                                                                                  cgcgCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGgtggtg                   >  1:89372/1‑140 (MQ=255)                                                                                                                    cgcACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCTACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGcc                 <  2:506447/140‑1 (MQ=255)                                                                                                                    cgcACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGcc                 >  2:162656/1‑140 (MQ=255)                                                                                                                      cACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCga               >  2:68198/1‑140 (MQ=255)                                                                                                                          aaGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCg                >  2:249818/1‑135 (MQ=255)                                                                                                                          aaGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCg                <  1:249818/135‑1 (MQ=255)                                                                                                                          aaGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATc           >  2:1244/1‑140 (MQ=255)                                                                                                                             gCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCcac        <  2:488161/140‑1 (MQ=255)                                                                                                                               aCGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACAt      <  1:220848/140‑1 (MQ=255)                                                                                                                                  cTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCgg   <  1:656304/140‑1 (MQ=255)                                                                                                                                  cTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCgg   <  2:89372/140‑1 (MQ=255)                                                                                                                                   tGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGt  <  1:538642/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                            CCATCCGCCACATCAAGGGTCAGCGGTTTAATGACCTGGGTTTTACCATCCCAGCTTTTGGTTACTGCCTGTAATTTCAGTCCTGCCATCTTATTTCTCACTATCGACCAGGCCGCGCACGAAGGCACGCTGCATCACTAAAACAATCACCACCGGAGGGATAAGCGTTAACAACATCGCCACCATCACTGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGT  >  NC_000913/3589092‑3589361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.