breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,604,344 | 0 | C | T | 41.1% | 182.7 / 114.9 | 163 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 3,556,205 | 0 | A | C | 39.3% | 87.4 / 31.0 | 61 | S215R (AGT→AGG) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 1,825,061 | 0 | G | A | 37.3% | 75.6 / 32.0 | 59 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
* | NC_000913 | 3,556,207 | 0 | T | C | 37.1% | 82.5 / 28.2 | 62 | S215G (AGT→GGT) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 4,111,747 | 0 | G | T | 35.6% | 180.6 / 59.3 | 87 | N156K (AAC→AAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 753,125 | 0 | T | A | 32.4% | 199.6 / 57.7 | 108 | intergenic (‑330/+60) | ybgD/gltA | putative fimbrial protein YbgD/citrate synthase |
* | NC_000913 | 2,001,918 | 0 | A | C | 28.0% | 145.6 / 33.3 | 75 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
* | NC_000913 | 445,558 | 0 | A | C | 27.8% | 136.9 / 36.4 | 72 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
* | NC_000913 | 650,809 | 0 | T | G | 27.6% | 173.3 / 29.7 | 87 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
* | NC_000913 | 4,296,060 | 0 | C | T | 27.2% | 335.9 / 127.7 | 180 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 3,206,435 | 0 | C | T | 26.9% | 129.5 / 30.7 | 67 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 646,747 | 0 | T | G | 26.7% | 186.3 / 41.4 | 90 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
* | NC_000913 | 753,114 | 0 | G | T | 26.2% | 286.3 / 28.5 | 107 | intergenic (‑319/+71) | ybgD/gltA | putative fimbrial protein YbgD/citrate synthase |
* | NC_000913 | 4,568,504 | 0 | T | G | 25.8% | 381.0 / 21.6 | 179 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
* | NC_000913 | 372,640 | 0 | T | G | 24.3% | 250.9 / 23.6 | 111 | L176V (TTA→GTA) | mhpD | 2‑hydroxypentadienoate hydratase |
* | NC_000913 | 2,628,625 | 0 | A | C | 23.8% | 144.8 / 18.6 | 63 | V105G (GTC→GGC) | pdeF | cyclic di‑GMP phosphodiesterase PdeF |
* | NC_000913 | 4,429,984 | 0 | T | G | 23.8% | 243.7 / 35.8 | 105 | L41V (TTG→GTG) | cycA | serine/alanine/glycine/cycloserine:H(+)symporter |
* | NC_000913 | 1,906,769 | 0 | A | C | 23.3% | 212.7 / 13.0 | 90 | Y98D (TAT→GAT) | rlmA | 23S rRNA m(1)G745 methyltransferase |
* | NC_000913 | 3,206,444 | 0 | T | A | 23.1% | 144.8 / 11.2 | 65 | intergenic (‑188/‑19) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 4,287,719 | 0 | T | A | 23.1% | 326.2 / 59.8 | 143 | intergenic (‑348/‑45) | acs/nrfA | acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 4542690 | 123 (0.740) | 34 (0.220) | 26/258 | 3.1 | 21.3% | intergenic (+57/‑425) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | = 4542986 | 136 (0.880) | intergenic (+353/‑129) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | 4542682 = | 140 (0.850) | 20 (0.130) | 16/258 | 4.7 | 13.0% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 136 (0.880) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | 374922 = | NA (NA) | 29 (0.190) | 8/260 | 6.9 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377329 = | NA (NA) | intergenic (+18/+206) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 374963 | NA (NA) | 4 (0.040) | 4/190 | 7.2 | NA | noncoding (33/34 nt) | other | REP31a |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 375059 | NA (NA) | 7 (0.040) +T |
6/274 | 7.8 | NA | noncoding (28/34 nt) | other | REP31c |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | 374922 = | NA (NA) | 5 (0.040) | 3/202 | 8.0 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377430 = | NA (NA) | intergenic (+119/+105) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 375246 | NA (NA) | 7 (0.050) +GAACGCCTTATCCGGT |
4/244 | 8.3 | NA | noncoding (13/34 nt) | other | REP31g |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | = 375160 | NA (NA) | 5 (0.030) +T |
4/274 | 8.8 | NA | noncoding (28/34 nt) | other | REP31e |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d |