Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,104,153	IS1 (–) +9 bp	coding (349‑357/1119 nt)	wbbK ←	putative lipopolysaccharide biosynthesis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	54 (1.160)	41/278	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 2104161	0 (0.000)					coding (349/1119 nt)	wbbK	putative lipopolysaccharide biosynthesis protein
*	?	NC_000913	= 1979270	NA (NA)	38 (0.820)	32/278	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	2104153 =	0 (0.000)					coding (357/1119 nt)	wbbK	putative lipopolysaccharide biosynthesis protein

GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979134‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACA  >  NC_000913/2104153‑2104288                                                                                                                                                                                                                                                                                   GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAA                                                                                                                                       <  1:12024/140‑1  ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAA                                                                                                                                      >  1:618084/1‑140        GGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCT                                                                                                                                <  2:463809/140‑1         GCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTT                                                                                                                               >  2:275298/1‑140                      CGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGC                                                                                                                  <  2:512286/140‑1                      CGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGC                                                                                                                  <  1:366252/140‑1                         TACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGG                                                                                                               >  1:142175/1‑140                             CACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTA                                                                                                           <  2:392423/140‑1                             CACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTA                                                                                                           <  2:225044/140‑1                                CACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGA                                                                                                        <  1:370081/140‑1                                     CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATA                                                                                                   >  1:428860/1‑140                                      GTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAC                                                                                                  >  2:220818/1‑140                                          GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACAT                                                                                              >  2:349048/1‑140                                             GCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATC                                                                                           >  1:347733/1‑140                                                      GAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAG                                                                                  >  1:4784/1‑140                                                           ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACG                                                                             >  1:427544/1‑140                                                                TGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATT                                                                        <  2:427544/140‑1                                                                     GAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCG                                                                   >  2:257179/1‑140                                                                         CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAAT                                                               <  2:72937/140‑1                                                                            AAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATC                                                            >  2:548517/1‑140                                                                               CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATG                                                         <  2:4784/140‑1                                                                               CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATG                                                         <  1:386593/140‑1                                                                               CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATG                                                         <  2:347733/140‑1                                                                                    GGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGAC                                                    >  1:290930/1‑140                                                                                        CACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAAT                                                >  2:343623/1‑140                                                                                          CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCC                                              >  2:686321/1‑140                                                                                          CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCC                                              >  2:660600/1‑140                                                                                                  ACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTA                                      >  1:95646/1‑140                                                                                                     CCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTA                                      >  1:230805/1‑137                                                                                                     CCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTA                                      <  2:230805/137‑1                                                                                                        TCATACACTAAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTC                                >  1:666963/1‑140                                                                                                                 AAATCAGTAAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTGATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTT                       >  2:102159/1‑140                                                                                                                         AAGTTGGCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTT               >  2:588047/1‑140                                                                                                                               GCAGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACA         <  1:131075/140‑1                                                                                                                                 AGCATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAAACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAA       <  1:349048/140‑1                                                                                                                                   CATCACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACT     >  1:728439/1‑140                                                                                                                                      CACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACA  >  2:319236/1‑140                                                                                                                                      CACCTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACA  >  2:741860/1‑140                                                                                                                                                                                                                                                                                   GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979134‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACA  >  NC_000913/2104153‑2104288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.