Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,581,699	(19-bp)2→1	intergenic (+60/+17)	tktB → / ← ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2581699	2581717	19	52 [0]	[0] 52	tktB/ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2581698	NA (NA)	50 (0.570)	41/238	0.8	NA	noncoding (36/36 nt)	other	REP179b
	?	NC_000913	2581718 =	NA (NA)					intergenic (+79/+16)	tktB/ypfG	transketolase 2/DUF1176 domain‑containing protein YpfG

GCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTG  >  NC_000913/2581586‑2581850                                                                                                                                     |                                                                                                                                     cccgttctttggctttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGc                                                                                                                               >  1:124190‑M2/133‑140 (MQ=255)      tctttggttttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCAttt                                                                                                                          <  2:1670194‑M2/13‑1 (MQ=255)         ttggctttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGt                                                                                                                       >  2:1231035‑M2/125‑140 (MQ=255)           ggctttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGttt                                                                                                                     <  2:1177599‑M2/18‑1 (MQ=255)              tttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAAc                                                                                                                  <  2:124190‑M2/21‑1 (MQ=255)               ttaccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACg                                                                                                                 >  2:346797‑M2/119‑140 (MQ=255)                 accgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACggg                                                                                                               <  1:1231035‑M2/24‑1 (MQ=255)                 accgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACggg                                                                                                               >  2:1339114‑M2/117‑140 (MQ=255)                 accgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACggg                                                                                                               >  1:207538‑M2/117‑140 (MQ=255)                  ccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgtcgctgacgcgtcttatccgccctacaTGTCCCGCCATTTTGTTTGACGGGt                                                                                                              <  2:571893‑M2/25‑1 (MQ=255)                  ccgccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGt                                                                                                              >  1:878550‑M2/116‑140 (MQ=255)                   cgccgagaatattgtggcaaaagcgcaaaaggtggtgggagtgaaaggtgcctgatggtgattgccggatgttgattgccggatgcgacgcggacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTg                                                                                                             <  2:695170‑M2/26‑1 (MQ=255)                    gccgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGa                                                                                                            <  1:1339114‑M2/27‑1 (MQ=255)                      cgagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATc                                                                                                          >  1:1326356‑M2/112‑140 (MQ=255)                       gagaatattgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATcc                                                                                                         <  1:643194‑M2/30‑1 (MQ=255)                             attgtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACg                                                                                                   <  1:617422‑M2/36‑1 (MQ=255)                                gtggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTgg                                                                                                <  1:792246‑M2/39‑1 (MQ=255)                                 tggcaaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTggg                                                                                               >  2:435978‑M2/101‑140 (MQ=255)                                    caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  1:1713315‑M2/98‑140 (MQ=255)                                    caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  1:1610103‑M2/98‑140 (MQ=255)                                    caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            <  2:1556688‑M2/43‑1 (MQ=255)                                    caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCa                                                                                            >  2:1083782‑M2/98‑140 (MQ=255)                                    caaaagcgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGGGGGCCa                                                                                            >  2:1390003‑M2/98‑140 (MQ=255)                                          cgcataaggtgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTc                                                                                      <  1:1614366‑M2/49‑1 (MQ=255)                                              aaaggtgcggggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGCCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGc                                                                                  <  2:772994‑M2/53‑1 (MQ=255)                                                   tgctgggagtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATg                                                                             >  2:1308683‑M2/83‑140 (MQ=255)                                                           gtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     <  2:1610103‑M2/66‑1 (MQ=255)                                                           gtgaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     >  1:336402‑M2/75‑140 (MQ=255)                                                             gaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     <  2:865632‑M2/66‑1 (MQ=255)                                                             gaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                     >  1:865632‑M2/73‑138 (MQ=255)                                                             gaaaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCg                                                                   <  1:435978‑M2/68‑1 (MQ=255)                                                               aaggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGAGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCa                                                                 >  2:1092512‑M2/71‑140 (MQ=255)                                                                aggtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAg                                                                <  2:767608‑M2/71‑1 (MQ=255)                                                                  gtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGt                                                              <  2:1534959‑M2/73‑1 (MQ=255)                                                                  gtgcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGt                                                              <  1:1308683‑M2/73‑1 (MQ=255)                                                                    gcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCg                                                                   >  1:318908‑M2/66‑133 (MQ=255)                                                                    gcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCg                                                                   <  2:318908‑M2/68‑1 (MQ=255)                                                                    gcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTgg                                                            >  1:1098634‑M2/66‑140 (MQ=255)                                                                    gcctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTgg                                                            >  2:280483‑M2/66‑140 (MQ=255)                                                                     cctgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTggg                                                           <  2:207538‑M2/76‑1 (MQ=255)                                                                       tgatggtgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGtt                                                         >  2:561940‑M2/63‑140 (MQ=255)                                                                             tgattgccggatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcgg                                                   <  2:1517899‑M2/84‑1 (MQ=255)                                                                                      gatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCa                                          <  2:878550‑M2/93‑1 (MQ=255)                                                                                      gatgctgattgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCa                                          >  1:371895‑M2/48‑140 (MQ=255)                                                                                             attgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCg                                   <  2:1326356‑M2/100‑1 (MQ=255)                                                                                             attgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCg                                   >  1:667170‑M2/41‑140 (MQ=255)                                                                                              ttgccggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGa                                  >  2:1412982‑M2/40‑140 (MQ=255)                                                                                               tgccggatgcggcgctgccgggtcttatggggcttacaGGCCACGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaa                                 <  1:165211‑M2/102‑1 (MQ=255)                                                                                                  cggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAAc                              >  1:1092230‑M2/36‑140 (MQ=255)                                                                                                   ggatgcgacgctgacgcgtcttatccggcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACg                             <  1:1008392‑M2/106‑1 (MQ=255)                                                                                                                              gcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg  >  1:978939‑M2/8‑140 (MQ=255)                                                                                                                              gcctacaTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg  >  1:481799‑M2/8‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     GCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTG  >  NC_000913/2581586‑2581850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑