breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 99,380 G→T A326A (GCG→GCT ftsW → putative lipid II flippase FtsW
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,474,566 G→T G116W (GGG→TGG)  pdxI → pyridoxine 4‑dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,469,892 G→T M254I (ATG→ATT yfdI → CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
RA 2,880,963 C→A R29I (AGA→ATA)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 3,022,708 G→T G465W (GGG→TGG)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,582,868 G→T D336Y (GAT→TAT)  yhhZ → putative endonuclease YhhZ
RA 3,601,453 C→A A212S (GCC→TCC)  ftsX ← cell division protein FtsX
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,884,900 C→A P29T (CCG→ACG)  yidD → membrane protein insertion efficiency factor
RA 4,139,931 G→T A764E (GCG→GAG)  ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,209,679 C→A noncoding (1533/1542 nt) rrsE → 16S ribosomal RNA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,332,541 G→T D244E (GAC→GAA basS ← sensory histidine kinase BasS
RA 4,338,429 G→T P698Q (CCG→CAG)  adiA ← arginine decarboxylase, degradative

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102971 3104051 1081 22 [20] [14] 24 [mutY] [mutY]
* * ÷ NC_000913 3423755–3424233 3424526–3424239 7–772 24 [19] [20] 22 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =35 (0.410)80 (1.050) 53/244 0.2 67.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 48 (0.630)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780536 (0.420)38 (0.500) 33/244 0.9 48.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 48 (0.630)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994981 (0.010)138 (1.650) 67/268 0.1 99.7% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =108 (1.260)114 (1.340) 52/274 0.3 67.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)90 (1.050) 65/276 0.1 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA