| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 4,518,060 | Δ7 bp | coding (170‑176/522 nt) | fecI ← | RNA polymerase sigma factor FecI |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 4518060 | 4518066 | 7 | 38 [0] | [0] 38 | fecI | RNA polymerase sigma factor FecI |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 4518059 | 0 (0.000) | 35 (0.680) | 25/264 | 0.8 | 100% | coding (177/522 nt) | fecI | RNA polymerase sigma factor FecI |
| ? | NC_000913 | 4518067 = | 0 (0.000) | coding (169/522 nt) | fecI | RNA polymerase sigma factor FecI | |||||
AAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGA > NC_000913/4517938‑4518198 | aaGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATGGtcgagagcgtttcgctga > 1:195787‑M1/1‑122 (MQ=255)aaGACGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCATATACGCTTTTTCGAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctga > 2:401656‑M1/1‑122 (MQ=255) ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgacc > 2:835698‑M1/1‑120 (MQ=255) ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcg < 1:483993‑M1/129‑10 (MQ=255) ggCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcg > 2:483993‑M1/1‑120 (MQ=255) cTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAGGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccatta > 1:324227‑M1/1‑116 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattac > 2:508178‑M1/1‑115 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattac > 1:19160‑M1/1‑115 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattac > 2:726340‑M1/1‑115 (MQ=255) tCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattac > 1:886001‑M1/1‑115 (MQ=255) tCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaa > 1:879500‑M1/1‑108 (MQ=255) cGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaa > 1:452747‑M1/1‑106 (MQ=255) ggCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaa < 2:509755‑M1/140‑36 (MQ=255) ggCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaa < 1:208933‑M1/140‑36 (MQ=255) aaGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCAGGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtc < 2:324227‑M1/140‑41 (MQ=255) aaGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtc < 2:783948‑M1/140‑41 (MQ=255) aaGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtc < 2:367966‑M1/140‑41 (MQ=255) gcCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgg < 1:726340‑M1/140‑45 (MQ=255) gcCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgg < 1:906469‑M1/140‑45 (MQ=255) ctcCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtc > 1:699150‑M1/1‑88 (MQ=255) gATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctg < 2:257452‑M1/140‑58 (MQ=255) aCGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcat > 2:292066‑M1/1‑80 (MQ=255) tttCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaag < 2:169349‑M1/140‑67 (MQ=255) tttCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaag < 1:232799‑M1/140‑67 (MQ=255) gTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagcca < 1:292066‑M1/140‑92 (MQ=255) aCGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaac < 2:19160‑M1/140‑102 (MQ=255) aCGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaac > 1:462589‑M1/1‑39 (MQ=255) cgcgTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaacc > 1:329312‑M1/1‑38 (MQ=255) ttGGCGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccg > 2:877526‑M1/1‑33 (MQ=255) cGATAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgat < 2:462589‑M1/140‑112 (MQ=255) tAGTGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtg < 2:699150‑M1/140‑115 (MQ=255) tGCAGAGGAAGGAGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgc > 1:275457‑M1/1‑23 (MQ=255) ggaGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcga > 1:616943‑M1/1‑13 (MQ=255) aGCGAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcgact > 2:103173‑M1/1‑11 (MQ=255) gAGGATCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcgactcga > 2:50824‑M1/1‑8 (MQ=255) aTCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcgactcgaacgt > 1:476767‑M1/1‑4 (MQ=255) aTCGtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcgactcgaacgt > 2:760628‑M1/1‑4 (MQ=255) gtcgagagcgtttcgctgaccattacccgcaaaaaagtgtcctgggcaatgtcatctgcatcaaaagcagactggagtttgcgcgtcagccagcttttcaaccagccgtgatgtgtgccataaagcgactcgaacgttaa < 2:616943‑M1/140‑140 (MQ=255) | AAGGCGCTCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGA > NC_000913/4517938‑4518198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |