Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,518,060	Δ7 bp	coding (170‑176/522 nt)	fecI ←	RNA polymerase sigma factor FecI

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4518060	4518066	7	38 [0]	[0] 38	fecI	RNA polymerase sigma factor FecI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4518059	0 (0.000)	35 (0.680)	25/264	0.8	100%	coding (177/522 nt)	fecI	RNA polymerase sigma factor FecI
	?	NC_000913	4518067 =	0 (0.000)					coding (169/522 nt)	fecI	RNA polymerase sigma factor FecI

TCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAA  >  NC_000913/4517945‑4518205                                                                                                                           |                                                                                                                                           aaggcgctcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatggTCGAGAGCGTTTCGCTGa                                                                                                                           >  1:195787‑M2/123‑140 (MQ=255) aagacgctcccccctccggcataagcgccagcatctccatatacgctttttcgagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGa                                                                                                                           >  2:401656‑M2/123‑140 (MQ=255)   ggcgctcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCg                                                                                                                                    <  1:483993‑M2/9‑1 (MQ=255)   ggcgctcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCg                                                                                                                                    >  2:483993‑M2/121‑129 (MQ=255)   ggcgctcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGAcc                                                                                                                         >  2:835698‑M2/121‑140 (MQ=255)       ctcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcaggcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTa                                                                                                                     >  1:324227‑M2/117‑140 (MQ=255)        tcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTAc                                                                                                                    >  2:726340‑M2/116‑140 (MQ=255)        tcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTAc                                                                                                                    >  2:508178‑M2/116‑140 (MQ=255)        tcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTAc                                                                                                                    >  1:886001‑M2/116‑140 (MQ=255)        tcccccctccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTAc                                                                                                                    >  1:19160‑M2/116‑140 (MQ=255)               tccggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCaaa                                                                                                             >  1:879500‑M2/109‑140 (MQ=255)                 cggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaa                                                                                                           >  1:452747‑M2/107‑140 (MQ=255)                  ggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaaa                                                                                                          <  2:509755‑M2/35‑1 (MQ=255)                  ggcataagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCaaaaaa                                                                                                          <  1:208933‑M2/35‑1 (MQ=255)                       aagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcaggcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTc                                                                                                     <  2:324227‑M2/40‑1 (MQ=255)                       aagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTc                                                                                                     <  2:783948‑M2/40‑1 (MQ=255)                       aagcgccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTc                                                                                                     <  2:367966‑M2/40‑1 (MQ=255)                           gccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTgg                                                                                                 <  1:726340‑M2/44‑1 (MQ=255)                           gccagcatctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTgg                                                                                                 <  1:906469‑M2/44‑1 (MQ=255)                                   ctccagatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTc                                                                                         >  1:699150‑M2/89‑140 (MQ=255)                                        gatacgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTg                                                                                    <  2:257452‑M2/57‑1 (MQ=255)                                           acgctttttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCAt                                                                                 >  2:292066‑M2/81‑140 (MQ=255)                                                 tttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAg                                                                           <  2:169349‑M2/66‑1 (MQ=255)                                                 tttccagcgcgtttcggcgaaacaggtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAg                                                                           <  1:232799‑M2/66‑1 (MQ=255)                                                                          gtccaccatcacgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTcagcca                                                  <  1:292066‑M2/91‑1 (MQ=255)                                                                                    acgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAAc                                        <  2:19160‑M2/101‑1 (MQ=255)                                                                                    acgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAAc                                        >  1:462589‑M2/40‑140 (MQ=255)                                                                                     cgcgtttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAAcc                                       >  1:329312‑M2/39‑140 (MQ=255)                                                                                          ttggcgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCg                                  >  2:877526‑M2/34‑140 (MQ=255)                                                                                              cgatagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGAt                              <  2:462589‑M2/111‑1 (MQ=255)                                                                                                 tagtgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGAtgtg                           <  2:699150‑M2/114‑1 (MQ=255)                                                                                                    tgcagaggaaggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGc                        >  1:275457‑M2/24‑140 (MQ=255)                                                                                                              ggagcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGa              >  1:616943‑M2/14‑140 (MQ=255)                                                                                                                agcgaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACt            >  2:103173‑M2/12‑140 (MQ=255)                                                                                                                   gaggatcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGa         >  2:50824‑M2/9‑140 (MQ=255)                                                                                                                       atcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGt     >  1:476767‑M2/5‑140 (MQ=255)                                                                                                                       atcgTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGt     >  2:760628‑M2/5‑140 (MQ=255)                                                                                                                          gTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTaa  <  2:616943‑M2/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                           TCCCCCCTCCGGCATAAGCGCCAGCATCTCCAGATACGCTTTTTCCAGCGCGTTTCGGCGAAACAGGTCCACCATCACGCGTTTGGCGATAGTGCAGAGGAAGGAGCGAGGATCGCGGATCGTCGAGAGCGTTTCGCTGACCATTACCCGCAAAAAAGTGTCCTGGGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAA  >  NC_000913/4517945‑4518205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑