Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,142,427	Δ257 bp	coding (1685‑1941/3186 nt)	rne ←	ribonuclease E

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1142427	1142683	257	35 [0]	[0] 35	rne	ribonuclease E

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1142426	0 (0.000)	32 (0.700)	25/264	0.7	100%	coding (1942/3186 nt)	rne	ribonuclease E
	?	NC_000913	1142684 =	0 (0.000)					coding (1684/3186 nt)	rne	ribonuclease E

GATCCTGTTGACGTTCCGGTTTCGCTTCTGCTTTCGGTGCTGGTTGCTCGGTCGGTTTGGTTTCTTCACCACCGCTGAACAGCGCTTTCAGTGCGCCGAAGAAGCGGCTCAACAGCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGAACT  >  NC_000913/1142546‑1142818                                                                                                                                           |                                                                                                                                       ttcttgttgcgcctgacgtttatcatcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctggt                                                                                                                                       <  2:416072‑M2/2‑1 (MQ=255)            cctgacgtttatcatcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGc                                                                                                                            >  2:395034‑M2/128‑140 (MQ=255)                acgtttatcatcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCt                                                                                                                        >  2:564677‑M2/124‑140 (MQ=255)                  gtttatcatcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCttt                                                                                                                      <  1:574591‑M2/19‑1 (MQ=255)                  gtttatcatcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCttt                                                                                                                      <  1:391956‑M2/19‑1 (MQ=255)                         atcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCt                                                                                                               <  2:323704‑M2/26‑1 (MQ=255)                         atcattacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCt                                                                                                               <  1:395034‑M2/26‑1 (MQ=255)                              tacggcggcggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGc                                                                                                          >  1:4733‑M2/110‑140 (MQ=255)                                       ggctacgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAgg                                                                                                 <  1:244294‑M2/40‑1 (MQ=255)                                           acgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGcgcg                                                                                             <  1:666198‑M2/44‑1 (MQ=255)                                            cgttcacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCgg                                                                                            <  1:536909‑M2/45‑1 (MQ=255)                                                cacgacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAgg                                                                                        <  2:485057‑M2/49‑1 (MQ=255)                                                   gacgcggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTc                                                                                     >  2:296001‑M2/89‑140 (MQ=255)                                                        ggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCgg                                                                                            <  1:45947‑M2/45‑1 (MQ=255)                                                        ggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCgg                                                                                            >  2:45947‑M2/84‑128 (MQ=255)                                                        ggcgcttgctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTg                                                                                >  1:507088‑M2/84‑140 (MQ=255)                                                               gctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTgg                                                                         >  1:787789‑M2/77‑140 (MQ=255)                                                               gctgctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAACTGGCGTTgg                                                                         >  2:734226‑M2/77‑140 (MQ=255)                                                                   ctcgtcggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCa                                                                     <  1:564677‑M2/68‑1 (MQ=255)                                                                         ggcggtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAggcggc                                                               <  1:296001‑M2/74‑1 (MQ=255)                                                                             gtacgcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGGTCAGCCGGCGGTGGCGCAGGCGGCGCAt                                                           >  2:124725‑M2/63‑140 (MQ=255)                                                                                 gcgctttttccgttacctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCgg                                                       <  2:4733‑M2/82‑1 (MQ=255)                                                                                               acctcagcctgctgacggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGGTg                                         >  1:175820‑M2/45‑140 (MQ=255)                                                                                                              cggctctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAACTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGgttgtt                          <  1:734226‑M2/111‑1 (MQ=255)                                                                                                                   ctcacgcgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGa                     <  1:124725‑M2/116‑1 (MQ=255)                                                                                                                         cgtctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTa               >  1:170902‑M2/19‑140 (MQ=255)                                                                                                                           tctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTATAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACg             >  2:447676‑M2/17‑140 (MQ=255)                                                                                                                           tctcggcagtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACg             >  1:188461‑M2/17‑140 (MQ=255)                                                                                                                                  agtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCg      <  1:740072‑M2/131‑1 (MQ=255)                                                                                                                                  agtctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCg      <  2:170902‑M2/131‑1 (MQ=255)                                                                                                                                    tctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTATAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGaa    <  1:447676‑M2/133‑1 (MQ=255)                                                                                                                                    tctgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGaa    >  1:774840‑M2/8‑140 (MQ=255)                                                                                                                                      tgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCg      >  1:674270‑M2/6‑136 (MQ=255)                                                                                                                                      tgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCg      <  2:674270‑M2/131‑1 (MQ=255)                                                                                                                                      tgctgGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGAAct  >  1:147860‑M2/6‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       GATCCTGTTGACGTTCCGGTTTCGCTTCTGCTTTCGGTGCTGGTTGCTCGGTCGGTTTGGTTTCTTCACCACCGCTGAACAGCGCTTTCAGTGCGCCGAAGAAGCGGCTCAACAGCCCAGGTTGTGCAGGAGCTGCTGGTGTTGCCGGTGCAGCTTTCGGTGCTGGAGCTACAACAGGCGCGGCAGGTTCAGCTGGCGTTGGCGCAGGCGGCACATCCGGCATGGCAAAGGTTGCCAGCGCAGGTTGTTCCGGACGCTTACGTTCAGCGAACT  >  NC_000913/1142546‑1142818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑