breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA BN000925 9,791 A→G D797G (GAC→GGC)  tnpA → Tn1 transposase
RA BN000925 9,802 G→A D801N (GAC→AAC)  tnpA → Tn1 transposase
RA BN000925 10,089 A→C A896A (GCA→GCC tnpA → Tn1 transposase
RA BN000925 10,359 T→A G986G (GGT→GGA tnpA → Tn1 transposase
RA BN000925 12,239 +A intergenic (‑601/+1055) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,406 T→G intergenic (‑768/+888) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,688 T→A intergenic (‑1050/+606) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 13,739 G→A P69L (CCG→CTG)  tetR ← tetracycline resistance repressor protein
RA BN000925 14,669 G→C R207P (CGG→CCG)  tetA → tetracycline exporter protein
RA BN000925 15,561 Δ1 bp intergenic (+312/+320) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,639 C→G intergenic (+390/+242) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,898 +C coding (649/666 nt) upf16.5 ← hypothetical protein
RA BN000925 16,410 +T coding (137/666 nt) upf16.5 ← hypothetical protein
RA BN000925 31,028 C→G P35A (CCC→GCC)  trbP → TrbP protein
RA BN000925 36,655 A→G R116G (AGA→GGA) 
R108G (AGA→GGA) 
istA →
istA →
IS21 transposase
IS21 transposase
RA BN000925 38,388 A→C pseudogene (45/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,390 1 bp→AG pseudogene (47/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,393 C→T pseudogene (50/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,421 Δ1 bp pseudogene (78/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,510 Δ1 bp pseudogene (167/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,526 Δ1 bp intergenic (+12/‑232) upf35.8 → / → aphA probable truncation of theoretical product due to sequence errors/aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,136 Δ1 bp coding (379/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,147 Δ1 bp coding (390/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,179 +CG coding (422/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 53,069 +T intergenic (+42/+10) traM → / ← kfrC TraM protein/KfrC protein
RA BN000925 54,833 Δ1 bp intergenic (‑32/+127) kfrB ← / ← kfrA KfrB protein/KfrA coiled‑coil protein
RA BN000925 56,817 Δ1 bp coding (297/522 nt) korF ← KorF repressor protein
MC JC NC_000913 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA NC_000913 486,014 C→A P85Q (CCA→CAA)  acrR → DNA‑binding transcriptional repressor AcrR
RA NC_000913 1,258,990 A→G E67G (GAA→GGA)  ychH → stress‑induced protein
RA NC_000913 1,296,375 A→G F583L (TTC→CTC)  adhE ← aldehyde‑alcohol dehydrogenase
RA NC_000913 1,298,371 C→A intergenic (‑250/‑227) adhE ← / → ychE aldehyde‑alcohol dehydrogenase/putative inner membrane protein
RA NC_000913 1,301,962 C→A R261S (CGC→AGC)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA NC_000913 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA NC_000913 2,249,587 A→T F11Y (TTT→TAT)  yeiE ← putative LysR‑type transcriptional regulator YeiE
MC JC NC_000913 3,366,751 Δ295 bp IS5‑mediated yhcE[yhcF] yhcE, [yhcF]
RA NC_000913 3,536,541 C→T R15H (CGC→CAC)  ompR ← DNA‑binding transcriptional dual regulator OmpR
RA NC_000913 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA NC_000913 3,717,751 C→T L148L (CTG→TTG)  ghrB → glyoxylate reductase
RA NC_000913 4,212,959 G→C intergenic (+13/‑81) rrlE → / → rrfE 23S ribosomal RNA/5S ribosomal RNA
RA NC_000913 4,212,973 Δ1 bp intergenic (+27/‑67) rrlE → / → rrfE 23S ribosomal RNA/5S ribosomal RNA
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423828–3424233 3424488–3424241 9–661 37 [36] [36] 37 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 8073360 (0.000)75 (0.680)
+G
37/428 2.1 61.6% intergenic (‑55/+97) ybhC/ybhB outer membrane lipoprotein YhbC/putative kinase inhibitor
?NC_000913 = 4171849 94 (0.850)intergenic (+93/‑208) rrfB/murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
* ? NC_000913 = 12994980 (0.000)115 (1.060) 76/422 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein