breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 188,837 C→T R316C (CGC→TGC)  PP_0165 → GGDEF domain‑containing protein
RA 233,545 C→T G548G (GGC→GGT PP_0180 → cytochrome c family protein
RA 476,133 A→T Q141L (CAG→CTG)  tsaD → tRNA N6‑adenosine(37)‑threonylcarbamoyltransferase complex transferase subunit TsaD
RA 591,535 T→A V260D (GTT→GAT)  PP_0501 → NAD‑dependent epimerase/dehydratase family protein
RA 1,011,323 +T intergenic (‑258/+192) PP_0871 ← / ← SBRG_Ecoli_galM glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
RA 1,164,101 G→A A427T (GCC→ACC)  gtsA → mannose/glucose ABC transporter substrate‑binding protein
RA 1,167,740 C→T Q185* (CAG→TAG)  oprB‑I → carbohydrate‑selective porin
RA 1,505,141 Δ1 bp coding (597/1095 nt) zapE → nucleoside triphosphate hydrolase domain‑containing protein
MC JC 1,515,811 (33-bp)2→1 pseudogene (715‑747/1851 nt) PP_1325 ← putative lipoprotein
RA 1,652,918 G→A Y265Y (TAC→TAT oprB‑II ← carbohydrate‑selective porin
RA 1,856,513 A→G D203G (GAT→GGT)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 2,043,352 (G)10→11 coding (1120/1143 nt) rffE ← UDP‑N‑acetylglucosamine‑2‑epimerase
RA 2,590,436 C→G Q17H (CAG→CAC folD‑II ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 3,138,381 G→A A195A (GCG→GCA PP_2750 → branched‑chain amino acid ABC transporter permease
RA 4,018,201 G→A intergenic (+144/‑36) mvaB → / → PP_mr44 hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA
RA 4,746,222 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,224 A→G intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,226 C→T intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,229 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,232 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,234 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,248 C→T intergenic (+490/+262) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,250 A→C intergenic (+492/+260) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,253 2 bp→GC intergenic (+495/+256) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 1015841 =21 (0.050)341 (0.840) 165/296 0.7 94.2% intergenic (‑68/‑700) SBRG_Ecoli_galE/prfC SBRG_Ecoli_galE/peptide chain release factor 3
?NC_002947 4541196 = NA (NA)intergenic (+730/‑85) PP_4022/PP_4024 hypothetical protein/transposase
* ? NC_002947 = 101584827 (0.070)380 (0.930) 168/296 0.6 93.4% intergenic (‑75/‑693) SBRG_Ecoli_galE/prfC SBRG_Ecoli_galE/peptide chain release factor 3
?NC_002947 = 4543236 NA (NA)intergenic (+45/‑275) PP_4025/PP_4027 transposase/hypothetical protein
* ? NC_002947 1444956 =NA (NA)412 (1.050) 177/286 0.2 100% intergenic (‑331/‑328) yveA/PP_1260 aspartate‑proton symporter/transposase
?NC_002947 = 1849442 0 (0.000)coding (343/2754 nt) gacS sensor protein GacS
* ? NC_002947 = 1446649NA (NA)368 (0.920) 159/290 0.8 100% intergenic (+37/+395) PP_1260/ghrB transposase/2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase
?NC_002947 1849440 = 0 (0.000)coding (345/2754 nt) gacS sensor protein GacS
* ? NC_002947 2930325 =333 (0.820)319 (0.800) 165/288 0.6 49.6% intergenic (‑81/+484) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
?NC_002947 = 2930764 NA (NA)intergenic (‑520/+45) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
* ? NC_002947 3976787 =NA (NA)396 (1.110) 164/260 0.2 100% intergenic (+177/‑203) PP_3501/PP_3502 transposase/transposase
?NC_002947 4578248 = 0 (0.000)intergenic (+63/‑74) PP_4054/glgX hypothetical protein/glycogen debranching enzyme
* ? NC_002947 = 45782490 (0.000)435 (1.120) 177/282 0.2 100% intergenic (+64/‑73) PP_4054/glgX hypothetical protein/glycogen debranching enzyme
?NC_002947 = 5763126 NA (NA)intergenic (+145/+373) PP_5050/PP_5051 transposase/hypothetical protein