breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPAL9_S9_L007_R1_001_val_1.good.fq5,548,936552,021,114100.0%99.5 bases100 bases95.4%
errorsPAL9_S9_L007_R2_001_val_2.good.fq5,518,216541,883,736100.0%98.2 bases100 bases95.0%
total11,067,1521,093,904,850100.0%98.8 bases100 bases95.2%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_000913_3_bme_pgi4,641,653222.42.1100.0%Escherichia coli str. K-12 substr. MG1655, B. melitensis pgi gene, complete genome.
total4,641,653100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100009
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50002169
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.098

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_000913_3_bme_pgi0.46054

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input18:19:34 19 Dec 202018:28:13 19 Dec 20208 minutes 39 seconds
Read alignment to reference genome18:28:14 19 Dec 202019:37:12 19 Dec 20201 hour 8 minutes 58 seconds
Preprocessing alignments for candidate junction identification19:37:12 19 Dec 202019:45:26 19 Dec 20208 minutes 14 seconds
Preliminary analysis of coverage distribution19:45:26 19 Dec 202020:00:46 19 Dec 202015 minutes 20 seconds
Identifying junction candidates20:00:46 19 Dec 202020:37:14 19 Dec 202036 minutes 28 seconds
Re-alignment to junction candidates20:37:14 19 Dec 202020:59:34 19 Dec 202022 minutes 20 seconds
Resolving best read alignments20:59:34 19 Dec 202021:09:48 19 Dec 202010 minutes 14 seconds
Creating BAM files21:09:48 19 Dec 202021:23:34 19 Dec 202013 minutes 46 seconds
Tabulating error counts21:23:34 19 Dec 202021:28:43 19 Dec 20205 minutes 9 seconds
Re-calibrating base error rates21:28:43 19 Dec 202021:28:46 19 Dec 20203 seconds
Examining read alignment evidence21:28:46 19 Dec 202022:30:46 19 Dec 20201 hour 2 minutes 0 seconds
Polymorphism statistics22:30:46 19 Dec 202022:31:00 19 Dec 202014 seconds
Output22:31:00 19 Dec 202022:34:20 19 Dec 20203 minutes 20 seconds
Total 4 hours 14 minutes 45 seconds