New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	3852834 =	43 (0.900)	6 (0.140)	6/260	5.2	13.7%	intergenic (‑50/+56)	ilvB/ivbL	acetolactate synthase 2 large subunit/ilvB operon leader peptide
	?	NC_000913_3_bme_pgi	3852867 =	36 (0.820)					intergenic (‑83/+23)	ilvB/ivbL	acetolactate synthase 2 large subunit/ilvB operon leader peptide
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAA  >  NC_000913_3_bme_pgi/3852724‑3853015                                                                                                                                                |                                                                                                                                                     taaCGATAAATTCTGCGCCGGTAAAGCGCGTACGCGTCTATGTGGTGCTCGAACTTGCCATGCTCCAGTCCTTTCCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATc                                                                                                                                                 <  2:24316/148‑1 (MQ=255) aaaCGATAAATTCTGTGCCGTTAAAGCTCTGAGGCGTTGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATc                                                                                                                                                 <  2:537329/148‑1 (MQ=255)  aaCGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGTACTTGCCATGGGCGCGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGGGGGGTTTTGGAATCg                                                                                                                                                >  1:408696/1‑149 (MQ=255)   aCGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGATGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCgt                                                                                                                                               <  1:555373/149‑1 (MQ=255)     gATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCgtgt                                                                                                                                             <  1:329052/149‑1 (MQ=255)             cTGCGCGGGTAAATCGCTTACGCGTCGGTGTTGTGCCAGAACTAGCGATGGTTCAGACCTTGTCTTCTGGGTCGCCTTTCCAGGGGGTGCTTAAACGAAAAGCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAAGCGTGTGTTGTTcc                                                                                                                                     <  2:451347/149‑1 (MQ=255)                 gcctggaaaGCGCTTACGCGTCGATGTTGTGCCCGAACTGGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTc                                                                                                                                 <  1:656001/143‑1 (MQ=255)                         aGCCCTTACGCGTTGATGTTGTGCCCGAACTTGCCGTGCTCCATTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGgc                                                                                                                         <  2:550329/149‑1 (MQ=255)                               tACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTg                                                                                                                   <  2:408696/149‑1 (MQ=255)                                  ggttCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCGTCTCCGCAGTAAATTGGTTAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCCGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCcg                                                                                                                <  1:406064/146‑1 (MQ=255)                                  gcgTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCcg                                                                                                                >  1:351206/1‑149 (MQ=255)                                      cGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCcgacga                                                                                                            <  2:572831/149‑1 (MQ=255)                                           ttgtGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGaccacc                                                                                                       <  1:486365/149‑1 (MQ=255)                                            tgtgCCCGAACTTGCTATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGaccacca                                                                                                      <  1:5617/149‑1 (MQ=255)                                               gCCCGAACTTGCCATCCTCCAGTCCTTTTCTTATGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCGCGTTCCTCCGGCGCATTGCCGCCGAccaccacca                                                                                                   >  2:594692/1‑149 (MQ=255)                                                 ccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGCAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTATGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCCACGACCCCCACcaca                                                                                                 >  2:171566/1‑149 (MQ=255)                                                  cGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCCCCACCacac                                                                                                >  1:206750/1‑149 (MQ=255)                                                           cATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTGAGGCGCATTGCCGCCGACCACCACCACACGCAcggcga                                                                                       >  2:110237/1‑149 (MQ=255)                                                                     tctttttCTTCTGGGCCGACTTTCCGGTAGGTGGTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGCATCGTGTGTGGTTCCAGTCCCTACGGCGCATTGCCTACGACTTCCTCTACACGCACGACGACCACTgcggc                                                                             <  2:729679/146‑1 (MQ=255)                                                                        ttttCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCCCTCCGGCGGa                                                                          >  1:643955/1‑149 (MQ=255)                                                                         tttCTTCTGGGTCGACTTTCCGGGAGGTGCTTAAACGAAAATCTCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGGCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGAt                                                                         <  1:220885/149‑1 (MQ=255)                                                                          ttcgtcTCGGCGGATTTTCCGCGAGGTGCTAAAGCGAAAAACCCCGCCCGGTTGGGGCGGGCGGGGATTTGGAACCGTGTGTTGTGCCAGTCCCTACGGCGCAGTGCCGACGACCCCCACCACACGCACGACGACCACTGCGGCGGATg                                                                        <  2:682733/149‑1 (MQ=255)                                                                               ctGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCa                                                                   <  1:191721/149‑1 (MQ=255)                                                                                  gtccGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGtt                                                                <  1:711538/147‑1 (MQ=255)                                                                                  ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGtt                                                                >  1:439776/1‑149 (MQ=255)                                                                                  ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGACGACGACCACCACCACACGAACGTCCCCAGCTTTGGCGGATGgcgccggt                                                                >  2:172135/1‑145 (MQ=255)                                                                                    ccGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACCCGCACGACGACCACTGCGGCGGATGGCGCAGTTgg                                                              >  2:303260/1‑149 (MQ=255)                                                                                      gACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGTCGCAGTTGgta                                                            <  1:516388/149‑1 (MQ=255)                                                                                      gACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGGTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATCGCGCAGTTGgta                                                            >  2:570568/1‑149 (MQ=255)                                                                                              gggCGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGAAGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGtttt                                                    <  2:201054/149‑1 (MQ=255)                                                                                                    tGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTg                                              >  1:741103/1‑149 (MQ=255)                                                                                                       ttAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGc                                           >  1:582229/1‑149 (MQ=255)                                                                                                            cGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGGCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGGGCTGTTGGTGGTAGTTTTGCGTTGAGCATggg                                      >  2:292978/1‑148 (MQ=255)                                                                                                             gAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCCCCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGaa                                     <  1:607539/149‑1 (MQ=255)                                                                                                                    cccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCa                              <  1:60492/149‑1 (MQ=255)                                                                                                                     ccgcccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAt                             >  2:70097/1‑149 (MQ=255)                                                                                                                                 accgggcggGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgt                 >  2:13307/5‑149 (MQ=255)                                                                                                                                   cgggcggGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgttg               <  1:13307/147‑1 (MQ=255)                                                                                                                                        ggggTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGtttt          <  2:741103/149‑1 (MQ=255)                                                                                                                                          ggTTTGGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTtgt        <  2:242267/149‑1 (MQ=255)                                                                                                                                           gTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTtgtg       >  1:684592/1‑149 (MQ=255)                                                                                                                                             tttGGAATCGTGCGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCTGCGGATGGTGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGtt     <  1:252314/149‑1 (MQ=255)                                                                                                                                                ggAATCGGGTGTTGTTCCAGTCCCCAGGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGACCCTCCGGGTATGCATTGGGGACCTTGTTGGTTTTGTGTTTaa  <  2:392801/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAA  >  NC_000913_3_bme_pgi/3852724‑3853015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.