New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 198356173 (2.010)5 (0.140) 5/268 4.2 7.2% coding (1509/1515 nt) araG L‑arabinose ABC transporter ATPase
?NC_000913_3_KO_pgi = 1983596 60 (1.740)coding (1474/1515 nt) araG L‑arabinose ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACAAAAATGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGG  >  NC_000913_3_KO_pgi/1983415‑1983706
                                                                                                                                                  |                                                                                                                                                 
gACAAAAATGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAAc                                                                                                                                                 >  1:246176/1‑149 (MQ=255)
 aCAAAAATGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACa                                                                                                                                                <  2:263101/149‑1 (MQ=255)
    aaaaTGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATCATCACCTTACTCAGGCAACAGcc                                                                                                                                             >  1:83216/1‑149 (MQ=255)
     aaaTGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCt                                                                                                                                            <  1:37980/149‑1 (MQ=255)
     aaaTGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCt                                                                                                                                            <  1:367910/149‑1 (MQ=255)
            acaGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCGGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCACCAGCCTGGCTGAc                                                                                                                                     >  1:299356/1‑149 (MQ=255)
            acaGGCGATAAAGAGCACCGCAAACACAACCAGCATGCCGTACTGATCCCAGATCCGACCAGAGCTGAATGACGACTTAGGTTCGCCAAACCCCTATGTAGAAACAGAAGACAGCATACTCTCCTTACACATTCAACAGCCTGCCTGAc                                                                                                                                     >  1:379568/1‑149 (MQ=255)
            acaGGCGATAAAGAGAACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCACGAAGCTGAATGACGACTTAGGTGCGCCCGAACCCGAAGTAGGAACAGGAGACATCATCCACGCCTGTCTCAGGCAACAGCCTGGCCGAc                                                                                                                                     >  2:267846/1‑149 (MQ=255)
                 cGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACACCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAg                                                                                                                                <  1:500651/149‑1 (MQ=255)
                  gATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATAATACTCGCCTTGCTCAGGCAACAGCCTGGCTGACTTTAgg                                                                                                                               >  1:496880/1‑149 (MQ=255)
                   aTAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTCGGTGCGCCAGACCTCGAAGTAGAAACAGAAGACATCATAGTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGc                                                                                                                              >  2:588790/1‑149 (MQ=255)
                    tAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCAAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTAAGGCAACAGCCTGGCTGACTTTAGGCa                                                                                                                             >  1:240334/1‑149 (MQ=255)
                     aaaGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCAt                                                                                                                            >  2:376578/1‑149 (MQ=255)
                           cACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAg                                                                                                                      >  2:613951/1‑149 (MQ=255)
                              cGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGACGTAGAAACAGAAGCCATCATACTCTCCTTACTCAGACAACAGCCTGGCTGCCTTTACGCATCGCAAGACt                                                                                                                   >  2:531841/1‑149 (MQ=255)
                                cAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCa                                                                                                                 <  2:373117/149‑1 (MQ=255)
                                cAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCa                                                                                                                 <  2:210415/149‑1 (MQ=255)
                                cAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCa                                                                                                                 >  1:373117/1‑149 (MQ=255)
                                  aaCACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAg                                                                                                                                >  2:117232/1‑132 (MQ=255)
                                  aaCACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAg                                                                                                                                <  1:117232/132‑1 (MQ=255)
                                   acacCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGGATCGCAAGGCTCAGTg                                                                                                              <  1:556678/149‑1 (MQ=255)
                                    caccacCAGGCTGGCGTACTGATCCCAGATACGTCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGc                                                                                                             <  2:592375/149‑1 (MQ=255)
                                     accaccAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGcc                                                                                                            >  1:115820/1‑149 (MQ=255)
                                       caccaGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAACCAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTg                                                                                                          >  2:427625/1‑149 (MQ=255)
                                          caGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACg                                                                                                       <  1:613951/149‑1 (MQ=255)
                                           aGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGc                                                                                                      <  2:555538/149‑1 (MQ=255)
                                             cATGCCGTATTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTc                                                                                                    >  2:101497/1‑149 (MQ=255)
                                                 ccGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTCCCTGACGCTCATca                                                                                                >  2:160905/1‑148 (MQ=255)
                                                  cGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTGTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATctg                                                                                               >  1:598456/1‑149 (MQ=255)
                                                   gTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCCTACTCAGCCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATctgc                                                                                              <  1:502819/149‑1 (MQ=255)
                                                            ccAGACACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCCACCGCCTTTCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCgtg                                                                                     <  1:601625/149‑1 (MQ=255)
                                                               gATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTaa                                                                                  <  1:106832/149‑1 (MQ=255)
                                                               gATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAAAACCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTaa                                                                                  <  1:153863/149‑1 (MQ=255)
                                                                  aCGCCCGAAGCTGAATGACGCCTTAGTTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACaa                                                                               <  2:287224/149‑1 (MQ=255)
                                                                   cGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAAt                                                                              <  2:115820/149‑1 (MQ=255)
                                                                      ccGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGAc                                                                                                        >  1:632826/1‑120 (MQ=255)
                                                                      ccGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGAc                                                                                                        <  2:632826/120‑1 (MQ=255)
                                                                      ccGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCa                                                                           >  1:227624/1‑149 (MQ=255)
                                                                         aaGCTGAATGACGACTTAGGTGCGCCAGACCCCGATTTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCg                                                                        <  1:53869/149‑1 (MQ=255)
                                                                         aaGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCg                                                                        <  1:54088/149‑1 (MQ=255)
                                                                          aGCAGAACGACTACTTAGGTGCGCCAGAACCCGAGGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTACGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCgg                                                                       <  2:14374/149‑1 (MQ=255)
                                                                              gAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGAt                                                                   >  1:209741/1‑149 (MQ=255)
                                                                               aaTGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGt                                                                                                               >  2:372457/1‑104 (MQ=255)
                                                                               aaTGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGt                                                                                                               <  1:372457/104‑1 (MQ=255)
                                                                               aaTGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGAtt                                                                  >  1:173391/1‑149 (MQ=255)
                                                                                        ttgggTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATCCTCTCCTTACGCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTcc                                                         <  1:531841/146‑1 (MQ=255)
                                                                                              gcgcCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATc                                                   <  1:72848/149‑1 (MQ=255)
                                                                                                  cAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATcacca                                               >  1:236908/1‑149 (MQ=255)
                                                                                                      ccccGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAAt                                           >  1:430029/1‑149 (MQ=255)
                                                                                                       cccGATGTAGAAAGAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCAGGCTGACTTTAGGCATCGCAAGGATCAGCGCCAGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCAGCTTCCCGCATCCCCCCAATc                                          >  1:523306/1‑149 (MQ=255)
                                                                                                       cccGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATc                                          >  2:70153/1‑149 (MQ=255)
                                                                                                         cGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCg                                        <  1:427625/149‑1 (MQ=255)
                                                                                                           aTGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAAGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTACCAGTTCACCAGCGATTTCACCTTCCCGCCTCACCACCATCCCGt                                      >  2:402613/1‑149 (MQ=255)
                                                                                                            tGTAGTAACAGAAGACATCAGACTCTCCGTACCCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTc                                     <  1:39052/149‑1 (MQ=255)
                                                                                                               aGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTa                                                                                                                                 <  2:553193/54‑1 (MQ=255)
                                                                                                               aGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTa                                                                                                                                 >  1:553193/1‑54 (MQ=255)
                                                                                                                  aaCAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAAc                               <  1:70153/149‑1 (MQ=255)
                                                                                                                      gaagaCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGa                                                                                                         <  2:113134/71‑1 (MQ=255)
                                                                                                                      gaagaCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGa                                                                                                         >  1:113134/1‑71 (MQ=255)
                                                                                                                      gaagaCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATGCACCGGCGACTTCACCTTCCCGCATCACCCCAATCTGGCCGGCCACGCCg                           >  2:233346/1‑149 (MQ=255)
                                                                                                                       aagaCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCCCAATCCGATCGGCAACGCCGa                          >  2:601427/1‑149 (MQ=255)
                                                                                                                       aagaCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGa                          >  2:118429/1‑149 (MQ=255)
                                                                                                                          aCATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGa                       <  2:173391/149‑1 (MQ=255)
                                                                                                                               atACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCa                  >  2:606204/1‑149 (MQ=255)
                                                                                                                               atACTCCCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCa                  <  2:593375/149‑1 (MQ=255)
                                                                                                                                tACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAg                 <  2:227624/149‑1 (MQ=255)
                                                                                                                                tACTCTCCTTACGCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAg                 <  2:389838/149‑1 (MQ=255)
                                                                                                                                     tcCTTACTCAGGCAACAGACTGGCTGACTTTAGGCATCGCAAGGCTTAGAGCCTGACGCTCATCTGCATGCCCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGCCTTCAGGTaaa            >  2:24663/1‑148 (MQ=255)
                                                                                                                                          aCTCAGGCAACAGCCTGGCTCACTTTAGGCATCGCAAGGCTCACTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACCATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCt       <  1:160905/149‑1 (MQ=255)
                                                                                                                                            tCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTgg     >  1:233695/1‑149 (MQ=255)
                                                                                                                                               ggCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAgg  <  2:598456/149‑1 (MQ=255)
                                                                                                                                                gCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAg   <  2:179661/147‑1 (MQ=255)
                                                                                                                                                gCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAg   >  1:179661/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACAAAAATGGCACAGGCGATAAAGAGCACCGCAAACACCACCAGCATGCCGTACTGATCCCAGATACGCCCGAAGCTGAATGACGACTTAGGTGCGCCAGACCCCGATGTAGAAACAGAAGACATCATACTCTCCTTACTCAGGCAACAGCCTGGCTGACTTTAGGCATCGCAAGGCTCAGTGCCTGACGCTCATCTGCCTGCTCGTGTAACAATTCACCGGCGATTTCACCTTCCCGCATCACCACAATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGG  >  NC_000913_3_KO_pgi/1983415‑1983706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.